BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0322 (738 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_21409| Best HMM Match : No HMM Matches (HMM E-Value=.) 50 2e-06 SB_23747| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.3 >SB_21409| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 195 Score = 50.0 bits (114), Expect = 2e-06 Identities = 46/152 (30%), Positives = 72/152 (47%), Gaps = 14/152 (9%) Frame = +3 Query: 147 LVTRAQG-DIQIEGFNPSAEEADEGTDSAVESGVDIVLNHRLVETYAFGDKKSYTLYLKD 323 +V+ QG D + G N SAE+A + + +G ++V+ +RLVE + K K Sbjct: 39 IVSEKQGIDENLIGGNKSAEDACDDVEDGCTTGCNVVMANRLVE-IPYKTFKELLAEFKP 97 Query: 324 YMKKLVAKL-EEKAPD-QVEVFKTNMNKVMKDILGRFKELQFFTGESMDCD-----GMVA 482 Y+KK+ + EE PD +V+ F+ + I FKE QFF DCD + Sbjct: 98 YIKKVKEHMKEEGCPDEEVKGFEKAAMDYILSIKKNFKEYQFF---QPDCDLGGGAHCIV 154 Query: 483 MMEY--RDFDGTQ----IPIMMFFKHGLEEEK 560 M Y +D G P++ FKH +++ K Sbjct: 155 FMWYPEKDLRGVDQDGFSPVLTVFKHAVKQVK 186 >SB_23747| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 853 Score = 29.9 bits (64), Expect = 2.3 Identities = 24/86 (27%), Positives = 40/86 (46%) Frame = +3 Query: 261 HRLVETYAFGDKKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKTNMNKVMKDILGRFKELQ 440 H+LVE +++ ++ +D LVA Q VF+ + N V K G K Sbjct: 734 HKLVEKRLVSNQQQRSIRFRDQRPHLVA--------QGVVFEPDANDVSK---GTLKVTG 782 Query: 441 FFTGESMDCDGMVAMMEYRDFDGTQI 518 F G+++ + +V + Y DF +QI Sbjct: 783 FVRGQTLSANRLVHLAGYGDFQISQI 808 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 21,626,196 Number of Sequences: 59808 Number of extensions: 413317 Number of successful extensions: 872 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 827 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 872 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1986074805 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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