BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0244 (686 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces p... 35 0.010 SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity... 34 0.022 SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Sc... 33 0.051 SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 32 0.089 SPCC1827.05c |||nucleolar RNA-binding protein NIFK |Schizosaccha... 31 0.21 SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 31 0.21 SPBC660.15 |||mRNA cleavage factor complex subunit |Schizosaccha... 29 0.63 SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 29 0.83 SPBC16E9.12c |pab2||poly|Schizosaccharomyces pombe|chr 2|||Manual 28 1.1 SPAC2F7.11 |nrd1|msa2|RNA-binding protein Nrd1|Schizosaccharomyc... 28 1.5 SPBC14C8.04 |||acetolactate synthase regulatory unit|Schizosacch... 28 1.5 SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces po... 27 1.9 SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces po... 27 2.5 SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces ... 27 3.4 SPAC1F7.11c |||transcription factor zf-fungal binuclear cluster ... 27 3.4 SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 26 4.4 SPBC13A2.01c |||nuclear cap-binding complex small subunit|Schizo... 26 4.4 SPAC1687.20c |mis6||inner centromere protein Mis6|Schizosaccharo... 26 5.9 >SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 517 Score = 35.1 bits (77), Expect = 0.010 Identities = 32/125 (25%), Positives = 55/125 (44%), Gaps = 15/125 (12%) Frame = +2 Query: 257 WDVPPPGFEHITPLQYK---------AMQAAGQIPANIV--ADTPQAAV----PVVGSTI 391 WD+ PPG+E +T Q K A +AA P ++ A + + ++ P + Sbjct: 135 WDIKPPGYELVTADQAKMSGVFPLPGAPRAAVTDPEKLLEFARSAEGSIIAPPPPLQPGA 194 Query: 392 TRQARRLYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRS 571 +RQARRL V IP E+ + F + + + + ++NFA LE + Sbjct: 195 SRQARRLVVTGIPNEFVEDAFVSFIEDLFISTTYHKPETKHFSSVNVCKEENFAILEVAT 254 Query: 572 IDETT 586 ++ T Sbjct: 255 PEDAT 259 >SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity factor complex subunit Ctf1|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 33.9 bits (74), Expect = 0.022 Identities = 23/68 (33%), Positives = 33/68 (48%) Frame = +2 Query: 410 LYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSIDETTQ 589 ++VGNIP+ V+E++ E FNQ + VL + K + F EF D T Sbjct: 9 VFVGNIPYDVSEQQMTEIFNQVGPVKTF-----KLVLDPETGSGKGYGFCEF--FDSETT 61 Query: 590 AMAFDGIN 613 AMA +N Sbjct: 62 AMAVRKLN 69 >SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Schizosaccharomyces pombe|chr 1|||Manual Length = 434 Score = 32.7 bits (71), Expect = 0.051 Identities = 21/72 (29%), Positives = 32/72 (44%) Frame = +2 Query: 380 GSTITRQARRLYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFL 559 G T+Q RR+YVGN+ + V E EF Q + +L L K A + Sbjct: 58 GQKYTQQERRVYVGNLSYQVRWFELKEFMGQ------VGNVLNCEILNLPNGLSKGCAII 111 Query: 560 EFRSIDETTQAM 595 E+ + +E A+ Sbjct: 112 EYSTAEEARTAI 123 >SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Schizosaccharomyces pombe|chr 2|||Manual Length = 388 Score = 31.9 bits (69), Expect = 0.089 Identities = 20/86 (23%), Positives = 42/86 (48%) Frame = +2 Query: 413 YVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSIDETTQA 592 +VGN+ F +TE + +FF + + L V+ + F ++EF + D + A Sbjct: 97 HVGNLSFDLTENDLGDFFGEGVTSIRL-------VIDPLTERSRGFGYVEFETADTLSAA 149 Query: 593 MAFDGINFKGQKLKDSTST*LPAYAR 670 +A G + G+ ++ + + ++AR Sbjct: 150 LALSGEDLMGRPVRITVAEPRRSFAR 175 >SPCC1827.05c |||nucleolar RNA-binding protein NIFK |Schizosaccharomyces pombe|chr 3|||Manual Length = 276 Score = 30.7 bits (66), Expect = 0.21 Identities = 17/56 (30%), Positives = 29/56 (51%) Frame = +2 Query: 410 LYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSID 577 LYVG +P G E++ +F+Q + L + + K++AF+EF S+D Sbjct: 107 LYVGRLPHGFYEKQMRMYFSQFGPVLRLRMSRNR-----KTGSSKHYAFIEFESLD 157 >SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 422 Score = 30.7 bits (66), Expect = 0.21 Identities = 21/68 (30%), Positives = 34/68 (50%) Frame = +2 Query: 410 LYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSIDETTQ 589 +YVGNIP+ + EE+ ++ F Q SG ++ V+ + K + F E+ D T Sbjct: 7 VYVGNIPYEMAEEQVIDIFKQ----SGPVKSF-QLVIDPESGQPKGYGFCEYH--DPATA 59 Query: 590 AMAFDGIN 613 A A +N Sbjct: 60 ASAVRNLN 67 >SPBC660.15 |||mRNA cleavage factor complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 474 Score = 29.1 bits (62), Expect = 0.63 Identities = 11/22 (50%), Positives = 15/22 (68%) Frame = +2 Query: 407 RLYVGNIPFGVTEEETMEFFNQ 472 +++VG +P TEEE FFNQ Sbjct: 248 KMFVGGVPGDCTEEEFRNFFNQ 269 >SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 28.7 bits (61), Expect = 0.83 Identities = 25/91 (27%), Positives = 47/91 (51%), Gaps = 1/91 (1%) Frame = +2 Query: 326 QIPANIVADTPQAAVPVVGSTITRQARRLYVGNIPFGVTEEETMEFF-NQQMHLSGLAQA 502 Q P++ + T + + P V + R L++GNIP +E +F+ ++ + LSG + Sbjct: 113 QTPSSEKSTTERESRPFVPPNSQQMRRMLFIGNIP-----KELDDFWMDKILRLSG--KL 165 Query: 503 AGNPVLACQINLDKNFAFLEFRSIDETTQAM 595 A +A N +F F EF S ++ ++A+ Sbjct: 166 ASWRRVADADNSMTSFGFAEFESNEQFSRAL 196 >SPBC16E9.12c |pab2||poly|Schizosaccharomyces pombe|chr 2|||Manual Length = 166 Score = 28.3 bits (60), Expect = 1.1 Identities = 21/79 (26%), Positives = 33/79 (41%), Gaps = 1/79 (1%) Frame = +2 Query: 401 ARRLYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQI-NLDKNFAFLEFRSID 577 A+ +YVGN+ + VT EE Q H + +L + K FA++EF Sbjct: 54 AQSVYVGNVDYSVTPEEL------QSHFASCGSVNRVTILCDKFTGHPKGFAYIEFSEPS 107 Query: 578 ETTQAMAFDGINFKGQKLK 634 A+ +G + LK Sbjct: 108 LVPNALLLNGSMLHERPLK 126 >SPAC2F7.11 |nrd1|msa2|RNA-binding protein Nrd1|Schizosaccharomyces pombe|chr 1|||Manual Length = 529 Score = 27.9 bits (59), Expect = 1.5 Identities = 21/75 (28%), Positives = 37/75 (49%) Frame = +2 Query: 404 RRLYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSIDET 583 R +++GN+P G+TE+E E + G P+ +I ++N AF+ F +I Sbjct: 206 RNVFLGNLPNGITEDEIRE----DLEPFG-------PIDQIKIVTERNIAFVHFLNIAAA 254 Query: 584 TQAMAFDGINFKGQK 628 +A+ +N K K Sbjct: 255 IKAVQELPLNPKWSK 269 >SPBC14C8.04 |||acetolactate synthase regulatory unit|Schizosaccharomyces pombe|chr 2|||Manual Length = 289 Score = 27.9 bits (59), Expect = 1.5 Identities = 12/34 (35%), Positives = 19/34 (55%) Frame = +2 Query: 290 TPLQYKAMQAAGQIPANIVADTPQAAVPVVGSTI 391 +P+ YKA+ A +P + + P+A VP S I Sbjct: 23 SPITYKALHANSPLPRCRIIEPPRATVPEAVSNI 56 >SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces pombe|chr 2|||Manual Length = 1014 Score = 27.5 bits (58), Expect = 1.9 Identities = 15/45 (33%), Positives = 20/45 (44%) Frame = +2 Query: 332 PANIVADTPQAAVPVVGSTITRQARRLYVGNIPFGVTEEETMEFF 466 P + P P G+ + R LYV NI F V E++ FF Sbjct: 735 PIQVFISDPLRRTPRSGAVY--EGRELYVTNIDFKVNEKDVETFF 777 >SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 365 Score = 27.1 bits (57), Expect = 2.5 Identities = 11/20 (55%), Positives = 14/20 (70%) Frame = +2 Query: 407 RLYVGNIPFGVTEEETMEFF 466 RL+VG IP T E+ M+FF Sbjct: 5 RLFVGRIPPQATREDMMDFF 24 >SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces pombe|chr 3|||Manual Length = 561 Score = 26.6 bits (56), Expect = 3.4 Identities = 17/59 (28%), Positives = 26/59 (44%) Frame = +2 Query: 302 YKAMQAAGQIPANIVADTPQAAVPVVGSTITRQARRLYVGNIPFGVTEEETMEFFNQQM 478 + A ++ I I A TP P + +YVGN+P +EEE F+ Q+ Sbjct: 395 FSAYSSSHGIHQRIPASTPTNTNPADQNP---PCNTIYVGNLPPSTSEEELKVLFSTQV 450 >SPAC1F7.11c |||transcription factor zf-fungal binuclear cluster type |Schizosaccharomyces pombe|chr 1|||Manual Length = 782 Score = 26.6 bits (56), Expect = 3.4 Identities = 11/26 (42%), Positives = 12/26 (46%) Frame = +3 Query: 366 PCRWWVRL*HVKREGYMSATYPLASP 443 P RWWV H G + A Y L P Sbjct: 550 PLRWWVFRFHSLNAGIIQAAYALCFP 575 >SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||Manual Length = 500 Score = 26.2 bits (55), Expect = 4.4 Identities = 21/90 (23%), Positives = 37/90 (41%) Frame = +2 Query: 380 GSTITRQARRLYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFL 559 G ++ + ++VGN+ F TE++ F G Q+ P Q K F ++ Sbjct: 358 GDQLSEPSDTVFVGNLSFNATEDDLSTAFGG----CGDIQSIRLPT-DPQSGRLKGFGYV 412 Query: 560 EFRSIDETTQAMAFDGINFKGQKLKDSTST 649 F ID + + +G G+ + ST Sbjct: 413 TFSDIDSAKKCVEMNGHFIAGRPCRLDFST 442 >SPBC13A2.01c |||nuclear cap-binding complex small subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 182 Score = 26.2 bits (55), Expect = 4.4 Identities = 10/26 (38%), Positives = 17/26 (65%) Frame = +2 Query: 395 RQARRLYVGNIPFGVTEEETMEFFNQ 472 +Q+ +YVGN+ F TEE+ F++ Sbjct: 29 KQSNCVYVGNLSFYTTEEQIYALFSK 54 >SPAC1687.20c |mis6||inner centromere protein Mis6|Schizosaccharomyces pombe|chr 1|||Manual Length = 672 Score = 25.8 bits (54), Expect = 5.9 Identities = 15/44 (34%), Positives = 19/44 (43%) Frame = +2 Query: 356 PQAAVPVVGSTITRQARRLYVGNIPFGVTEEETMEFFNQQMHLS 487 PQ + G I Q G+IP +EET F +HLS Sbjct: 597 PQFFSYINGYQIYLQQTNPATGSIPLKPIQEETFGAFQSNLHLS 640 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,646,085 Number of Sequences: 5004 Number of extensions: 55023 Number of successful extensions: 170 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 162 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 167 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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