BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0241 (505 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 69 3e-13 SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 48 8e-07 SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 43 2e-05 SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 42 5e-05 SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 32 0.056 SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 30 0.17 SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 29 0.30 SPBC23E6.05 |arx1||ribosomal export complex Arx1 |Schizosaccharo... 25 4.9 SPAC1071.10c |pma1||P-type proton ATPase Pma1 |Schizosaccharomyc... 25 6.5 SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|c... 25 8.5 >SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces pombe|chr 1|||Manual Length = 247 Score = 69.3 bits (162), Expect = 3e-13 Identities = 32/65 (49%), Positives = 43/65 (66%) Frame = +2 Query: 188 LAPLPIGICTSEGVVLAVGERITSTLMEPTTIEKIVEVDRHIGCAVSGLMGDFRTLFERA 367 L IG+ T + VVL V +R+TS LME ++EK+ E+D HIGCA+SGL D RT+ E A Sbjct: 33 LGSTAIGVKTKDAVVLGVEKRLTSPLMESHSVEKLFEIDSHIGCAISGLTADARTIIEHA 92 Query: 368 PVNVK 382 V + Sbjct: 93 RVQTQ 97 Score = 59.7 bits (138), Expect = 2e-10 Identities = 42/121 (34%), Positives = 57/121 (47%) Frame = +1 Query: 142 EGRLFQVEYAIEAIKLGSTANWHLYFGGSCFGRGRKDNFNFDGTHDH*KDC*SRSTHWLR 321 EGRLFQVEYAIEAIKLGSTA G ++ +H K S Sbjct: 18 EGRLFQVEYAIEAIKLGSTAIGVKTKDAVVLGVEKRLTSPLMESHSVEKLFEIDSHIGCA 77 Query: 322 RFGSDGRLQDLI*KGPSECQNHWFVYNERMSVESCAQALSNLTIQFGDTDDDSRTAMSPA 501 G + +I + QNH F Y+E +ES Q++ +L ++FG+ +D MS Sbjct: 78 ISGLTADARTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLALRFGEGEDGEERIMSRP 137 Query: 502 F 504 F Sbjct: 138 F 138 Score = 34.3 bits (75), Expect = 0.011 Identities = 14/14 (100%), Positives = 14/14 (100%) Frame = +3 Query: 99 TRSEYDRGVNTFSP 140 TRSEYDRGVNTFSP Sbjct: 4 TRSEYDRGVNTFSP 17 >SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharomyces pombe|chr 3|||Manual Length = 253 Score = 48.0 bits (109), Expect = 8e-07 Identities = 23/55 (41%), Positives = 36/55 (65%) Frame = +2 Query: 203 IGICTSEGVVLAVGERITSTLMEPTTIEKIVEVDRHIGCAVSGLMGDFRTLFERA 367 IGI +GV+LA+ + +TS L++P +I VDRHIG A +G + D + + +RA Sbjct: 37 IGIKCEDGVILALEKVVTSKLLKPRVNNRIGSVDRHIGIATTGFIPDGQHIVKRA 91 Score = 27.5 bits (58), Expect = 1.2 Identities = 11/19 (57%), Positives = 15/19 (78%) Frame = +1 Query: 142 EGRLFQVEYAIEAIKLGST 198 +GRLFQ EYA +A++ ST Sbjct: 17 DGRLFQAEYAYKAVENAST 35 >SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces pombe|chr 3|||Manual Length = 245 Score = 43.2 bits (97), Expect = 2e-05 Identities = 23/68 (33%), Positives = 37/68 (54%) Frame = +2 Query: 185 NLAPLPIGICTSEGVVLAVGERITSTLMEPTTIEKIVEVDRHIGCAVSGLMGDFRTLFER 364 N +GI ++GVVLA ++ TS L ++EK+ + IG SG+ DFR L ++ Sbjct: 29 NAGVTSVGIKATDGVVLATEKKPTSELAIGASLEKVCAITPDIGMVYSGMGPDFRVLVDK 88 Query: 365 APVNVKTT 388 + +TT Sbjct: 89 SRKVAQTT 96 Score = 25.4 bits (53), Expect = 4.9 Identities = 9/19 (47%), Positives = 14/19 (73%) Frame = +1 Query: 145 GRLFQVEYAIEAIKLGSTA 201 G+L Q+EYA+ A+ G T+ Sbjct: 16 GKLVQIEYALNAVNAGVTS 34 >SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 41.9 bits (94), Expect = 5e-05 Identities = 22/62 (35%), Positives = 36/62 (58%), Gaps = 1/62 (1%) Frame = +2 Query: 185 NLAPLPIGICTSEGVVLAVGERITSTLME-PTTIEKIVEVDRHIGCAVSGLMGDFRTLFE 361 N A + +GI +G+VLA +++TS L+E + EK+ + ++ CAV+GL D L Sbjct: 29 NHAGVALGIVAKDGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILIN 88 Query: 362 RA 367 A Sbjct: 89 YA 90 Score = 29.9 bits (64), Expect = 0.23 Identities = 13/20 (65%), Positives = 15/20 (75%) Frame = +1 Query: 142 EGRLFQVEYAIEAIKLGSTA 201 EGRL+QVEYA+EAI A Sbjct: 15 EGRLYQVEYALEAINHAGVA 34 >SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Schizosaccharomyces pombe|chr 1|||Manual Length = 272 Score = 31.9 bits (69), Expect = 0.056 Identities = 14/18 (77%), Positives = 16/18 (88%) Frame = +1 Query: 142 EGRLFQVEYAIEAIKLGS 195 +GRL QVEYA+EAIK GS Sbjct: 16 QGRLHQVEYALEAIKQGS 33 >SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 30.3 bits (65), Expect = 0.17 Identities = 12/62 (19%), Positives = 30/62 (48%) Frame = +2 Query: 182 SNLAPLPIGICTSEGVVLAVGERITSTLMEPTTIEKIVEVDRHIGCAVSGLMGDFRTLFE 361 +N +G+ + +++ L++ +T++ + + + IGC ++G + D R Sbjct: 31 NNAGITSVGVTGKNCACVISQKKVPDKLIDASTVKHVFPITKGIGCVMTGSIADARAQVS 90 Query: 362 RA 367 RA Sbjct: 91 RA 92 Score = 25.4 bits (53), Expect = 4.9 Identities = 10/13 (76%), Positives = 12/13 (92%) Frame = +1 Query: 142 EGRLFQVEYAIEA 180 EGRL+QVEYA +A Sbjct: 17 EGRLYQVEYAFKA 29 >SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces pombe|chr 2|||Manual Length = 259 Score = 29.5 bits (63), Expect = 0.30 Identities = 12/20 (60%), Positives = 16/20 (80%) Frame = +1 Query: 142 EGRLFQVEYAIEAIKLGSTA 201 +GRL QVEY EA++ G+TA Sbjct: 14 DGRLLQVEYGQEAVRRGTTA 33 >SPBC23E6.05 |arx1||ribosomal export complex Arx1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 417 Score = 25.4 bits (53), Expect = 4.9 Identities = 10/40 (25%), Positives = 21/40 (52%) Frame = -3 Query: 242 PRPKQLPPKYKCQLAVEPSLIASMAYSTWNSLPSRAECVD 123 P P++ K +++P+ + S +TW+ +P A +D Sbjct: 229 PAPEEETRKADVTNSLDPANVLSTELNTWHVMPKEAWLID 268 >SPAC1071.10c |pma1||P-type proton ATPase Pma1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 919 Score = 25.0 bits (52), Expect = 6.5 Identities = 11/21 (52%), Positives = 15/21 (71%) Frame = +2 Query: 206 GICTSEGVVLAVGERITSTLM 268 G+ T G+VLA+G IT+T M Sbjct: 755 GLSTVIGIVLAIGTWITNTTM 775 >SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 559 Score = 24.6 bits (51), Expect = 8.5 Identities = 11/23 (47%), Positives = 14/23 (60%) Frame = -3 Query: 143 SRAECVDPAIILGTCKTFYY*GY 75 S AE VDP+ + G K +YY Y Sbjct: 537 SNAELVDPSTLSGKDKFWYYIDY 559 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,198,609 Number of Sequences: 5004 Number of extensions: 48283 Number of successful extensions: 138 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 129 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 137 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 200198394 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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