Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0241
(505 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2) ... 80 7e-16
At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1) ... 80 7e-16
At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1) (... 51 5e-07
At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2) ... 44 6e-05
At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1) 44 6e-05
At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1) (... 44 8e-05
At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putativ... 42 2e-04
At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1) (... 42 2e-04
At4g15160.1 68417.m02327 protease inhibitor/seed storage/lipid t... 41 5e-04
At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1) ... 35 0.036
At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2) ... 35 0.036
At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1) ... 32 0.25
At5g07780.1 68418.m00890 formin homology 2 domain-containing pro... 31 0.44
At2g13680.1 68415.m01508 glycosyl transferase family 48 protein ... 30 0.77
At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2) ... 30 1.0
At4g37310.1 68417.m05283 cytochrome P450, putative 29 2.4
At1g06770.2 68414.m00719 zinc finger (C3HC4-type RING finger) fa... 28 3.1
At1g06770.1 68414.m00720 zinc finger (C3HC4-type RING finger) fa... 28 3.1
At2g32810.1 68415.m04016 beta-galactosidase, putative / lactase,... 28 4.1
At5g63800.1 68418.m08007 glycosyl hydrolase family 35 protein si... 27 7.2
At5g45470.1 68418.m05586 expressed protein contains Pfam domain,... 27 7.2
At1g77410.1 68414.m09015 beta-galactosidase, putative / lactase,... 27 9.5
>At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2)
identical to 20S proteasome subunit PAE2 GB:AAC32061
from [Arabidopsis thaliana]
Length = 237
Score = 80.2 bits (189), Expect = 7e-16
Identities = 38/65 (58%), Positives = 49/65 (75%)
Frame = +2
Query: 188 LAPLPIGICTSEGVVLAVGERITSTLMEPTTIEKIVEVDRHIGCAVSGLMGDFRTLFERA 367
L IG+ T EGVVLAV +RITS L+EP+++EKI+E+D HIGCA+SGL+ D RTL E A
Sbjct: 33 LGSTAIGVKTKEGVVLAVEKRITSPLLEPSSVEKIMEIDDHIGCAMSGLIADARTLVEHA 92
Query: 368 PVNVK 382
V +
Sbjct: 93 RVETQ 97
Score = 56.4 bits (130), Expect = 1e-08
Identities = 42/113 (37%), Positives = 54/113 (47%)
Frame = +1
Query: 142 EGRLFQVEYAIEAIKLGSTANWHLYFGGSCFGRGRKDNFNFDGTHDH*KDC*SRSTHWLR 321
EGRLFQVEYAIEAIKLGSTA G ++ K
Sbjct: 18 EGRLFQVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITSPLLEPSSVEKIMEIDDHIGCA 77
Query: 322 RFGSDGRLQDLI*KGPSECQNHWFVYNERMSVESCAQALSNLTIQFGDTDDDS 480
G + L+ E QNH F Y E M+VES QAL +L ++FG+ +++S
Sbjct: 78 MSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQALCDLALRFGEGEEES 130
Score = 33.1 bits (72), Expect = 0.11
Identities = 13/14 (92%), Positives = 14/14 (100%)
Frame = +3
Query: 99 TRSEYDRGVNTFSP 140
TR+EYDRGVNTFSP
Sbjct: 4 TRTEYDRGVNTFSP 17
>At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1)
identical to 20S proteasome subunit PAE1 GI:3421087 from
[Arabidopsis thaliana]
Length = 237
Score = 80.2 bits (189), Expect = 7e-16
Identities = 38/65 (58%), Positives = 49/65 (75%)
Frame = +2
Query: 188 LAPLPIGICTSEGVVLAVGERITSTLMEPTTIEKIVEVDRHIGCAVSGLMGDFRTLFERA 367
L IG+ T EGVVLAV +RITS L+EP+++EKI+E+D HIGCA+SGL+ D RTL E A
Sbjct: 33 LGSTAIGVKTKEGVVLAVEKRITSPLLEPSSVEKIMEIDDHIGCAMSGLIADARTLVEHA 92
Query: 368 PVNVK 382
V +
Sbjct: 93 RVETQ 97
Score = 56.4 bits (130), Expect = 1e-08
Identities = 42/113 (37%), Positives = 54/113 (47%)
Frame = +1
Query: 142 EGRLFQVEYAIEAIKLGSTANWHLYFGGSCFGRGRKDNFNFDGTHDH*KDC*SRSTHWLR 321
EGRLFQVEYAIEAIKLGSTA G ++ K
Sbjct: 18 EGRLFQVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITSPLLEPSSVEKIMEIDDHIGCA 77
Query: 322 RFGSDGRLQDLI*KGPSECQNHWFVYNERMSVESCAQALSNLTIQFGDTDDDS 480
G + L+ E QNH F Y E M+VES QAL +L ++FG+ +++S
Sbjct: 78 MSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQALCDLALRFGEGEEES 130
Score = 33.1 bits (72), Expect = 0.11
Identities = 13/14 (92%), Positives = 14/14 (100%)
Frame = +3
Query: 99 TRSEYDRGVNTFSP 140
TR+EYDRGVNTFSP
Sbjct: 4 TRTEYDRGVNTFSP 17
>At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1)
(PRC9) identical to GB:AAC32057 from [Arabidopsis
thaliana] (Genetics (1998) 149 (2), 677-692); identical
to cDNA proteasome subunit prc9 GI:2511583
Length = 250
Score = 50.8 bits (116), Expect = 5e-07
Identities = 24/61 (39%), Positives = 39/61 (63%), Gaps = 1/61 (1%)
Frame = +2
Query: 203 IGICTSEGVVLAVGERITSTLMEPTT-IEKIVEVDRHIGCAVSGLMGDFRTLFERAPVNV 379
IGI + +GVVL +++TS L++ +T EK+ ++D H+ CAV+G+M D L A V
Sbjct: 35 IGILSKDGVVLIGEKKVTSKLLQTSTSAEKMYKIDDHVACAVAGIMSDANILINTARVQA 94
Query: 380 K 382
+
Sbjct: 95 Q 95
Score = 29.1 bits (62), Expect = 1.8
Identities = 12/14 (85%), Positives = 14/14 (100%)
Frame = +1
Query: 142 EGRLFQVEYAIEAI 183
EGRL+QVEYA+EAI
Sbjct: 15 EGRLYQVEYAMEAI 28
>At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2)
(PRS1) (PRC6) identical to SP|O24616 Proteasome subunit
alpha type 7-2 (EC 3.4.25.1) (20S proteasome alpha
subunit D2) {Arabidopsis thaliana}
Length = 250
Score = 44.0 bits (99), Expect = 6e-05
Identities = 21/61 (34%), Positives = 35/61 (57%)
Frame = +2
Query: 203 IGICTSEGVVLAVGERITSTLMEPTTIEKIVEVDRHIGCAVSGLMGDFRTLFERAPVNVK 382
+G+ ++ VVLAV ++ T L + + KIV +D HI A +GL D R L +A + +
Sbjct: 34 VGVRGTDTVVLAVEKKSTPKLQDSRSARKIVSLDNHIALACAGLKADARVLINKARIECQ 93
Query: 383 T 385
+
Sbjct: 94 S 94
Score = 31.5 bits (68), Expect = 0.33
Identities = 12/20 (60%), Positives = 17/20 (85%)
Frame = +1
Query: 142 EGRLFQVEYAIEAIKLGSTA 201
+G LFQVEYA+EA++ G+ A
Sbjct: 14 DGHLFQVEYALEAVRKGNAA 33
>At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1)
Length = 250
Score = 44.0 bits (99), Expect = 6e-05
Identities = 21/61 (34%), Positives = 35/61 (57%)
Frame = +2
Query: 203 IGICTSEGVVLAVGERITSTLMEPTTIEKIVEVDRHIGCAVSGLMGDFRTLFERAPVNVK 382
+G+ ++ VVLAV ++ T L + + KIV +D HI A +GL D R L +A + +
Sbjct: 34 VGVRGTDTVVLAVEKKSTPKLQDSRSARKIVSLDNHIALACAGLKADARVLINKARIECQ 93
Query: 383 T 385
+
Sbjct: 94 S 94
Score = 31.5 bits (68), Expect = 0.33
Identities = 12/20 (60%), Positives = 17/20 (85%)
Frame = +1
Query: 142 EGRLFQVEYAIEAIKLGSTA 201
+G LFQVEYA+EA++ G+ A
Sbjct: 14 DGHLFQVEYALEAVRKGNAA 33
>At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1)
(PRC8) identical to proteasome subunit alpha type 3
SP:O23715, GI:12644056 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc8 GI:2511591
Length = 249
Score = 43.6 bits (98), Expect = 8e-05
Identities = 22/55 (40%), Positives = 32/55 (58%)
Frame = +2
Query: 203 IGICTSEGVVLAVGERITSTLMEPTTIEKIVEVDRHIGCAVSGLMGDFRTLFERA 367
+GI +G+V+ V + I S +M P + +I V RH G AV+GL D R + RA
Sbjct: 38 VGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHAGMAVAGLAADGRQIVARA 92
>At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putative
nearly identical to SP|O23708 Proteasome subunit alpha
type 2 (EC 3.4.25.1) (20S proteasome alpha subunit B)
{Arabidopsis thaliana} and to At1g16470
Length = 235
Score = 41.9 bits (94), Expect = 2e-04
Identities = 19/55 (34%), Positives = 34/55 (61%)
Frame = +2
Query: 203 IGICTSEGVVLAVGERITSTLMEPTTIEKIVEVDRHIGCAVSGLMGDFRTLFERA 367
+GI S GVV+A +++ S L++ +++KI + +IG SG+ DFR L ++
Sbjct: 36 LGIKASNGVVIATEKKLPSILVDEASVQKIQHLTPNIGTVYSGMGPDFRVLVRKS 90
>At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1)
(PRC3) identical to proteasome subunit alpha type 2
SP:O23708, GI:6093778; identical to cDNA proteasome
subunit prc3 GI:2511573
Length = 235
Score = 41.9 bits (94), Expect = 2e-04
Identities = 19/55 (34%), Positives = 34/55 (61%)
Frame = +2
Query: 203 IGICTSEGVVLAVGERITSTLMEPTTIEKIVEVDRHIGCAVSGLMGDFRTLFERA 367
+GI S GVV+A +++ S L++ +++KI + +IG SG+ DFR L ++
Sbjct: 36 LGIKASNGVVIATEKKLPSILVDEASVQKIQHLTPNIGVVYSGMGPDFRVLVRKS 90
>At4g15160.1 68417.m02327 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to
SP|Q00451|PRF1_LYCES 36.4 kDa proline-rich protein
Lycopersicon esculentum, proline-rich cell wall protein
[Medicago sativa] GI:3818416; contains Pfam profile
PF00234 Protease inhibitor/seed storage/LTP family
Length = 428
Score = 40.7 bits (91), Expect = 5e-04
Identities = 17/46 (36%), Positives = 32/46 (69%), Gaps = 1/46 (2%)
Frame = +2
Query: 197 LPIGICTSEGVVLAVGERITSTLMEPTT-IEKIVEVDRHIGCAVSG 331
LP+ +GVVL +++TS L++ ++ +EK+ ++D H+ CAV+G
Sbjct: 315 LPLEFLAKDGVVLVGEKKVTSKLLQTSSSMEKMYKIDDHVACAVAG 360
>At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1)
(gb|AAC32062.1)
Length = 278
Score = 34.7 bits (76), Expect = 0.036
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +1
Query: 145 GRLFQVEYAIEAIKLGSTA 201
GRLFQVEYA+EA+K GS A
Sbjct: 17 GRLFQVEYAMEAVKQGSAA 35
>At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2)
(PRC2B) (PRS1) identical to GB:AAC32063 from
[Arabidopsis thaliana] (Genetics 149 (2), 677-692
(1998)); identical to cDNA proteasome subunit prc2b
GI:2511585
Length = 277
Score = 34.7 bits (76), Expect = 0.036
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +1
Query: 145 GRLFQVEYAIEAIKLGSTA 201
GRLFQVEYA+EA+K GS A
Sbjct: 17 GRLFQVEYAMEAVKQGSAA 35
>At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1)
(PRC1) identical to proteasome subunit alpha type 6-1
SP:O81146 GI:12643647 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc1 GI:2511587
Length = 246
Score = 31.9 bits (69), Expect = 0.25
Identities = 11/55 (20%), Positives = 31/55 (56%)
Frame = +2
Query: 203 IGICTSEGVVLAVGERITSTLMEPTTIEKIVEVDRHIGCAVSGLMGDFRTLFERA 367
IG+ + V + +++ L++ +++ + + ++IG +G+ D R+L ++A
Sbjct: 40 IGVRGKDSVCVVTQKKVPDKLLDQSSVTHLFPITKYIGLVATGITADARSLVQQA 94
Score = 29.9 bits (64), Expect = 1.0
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +1
Query: 142 EGRLFQVEYAIEAIK 186
EGRLFQVEYA +A+K
Sbjct: 19 EGRLFQVEYAFKAVK 33
>At5g07780.1 68418.m00890 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 464
Score = 31.1 bits (67), Expect = 0.44
Identities = 18/43 (41%), Positives = 21/43 (48%), Gaps = 2/43 (4%)
Frame = -3
Query: 245 LPRPKQLPPKYKCQLAVEPSLIASMAY--STWNSLPSRAECVD 123
LPRP PPK KC L + + A S W+ L R EC D
Sbjct: 64 LPRPCSRPPKTKCSLKPLHWVKKTRALPGSLWDELQRRQECRD 106
>At2g13680.1 68415.m01508 glycosyl transferase family 48 protein
contains Pfam profile: PF02364 1,3-beta-glucan synthase
Length = 1923
Score = 30.3 bits (65), Expect = 0.77
Identities = 17/42 (40%), Positives = 28/42 (66%), Gaps = 4/42 (9%)
Frame = -3
Query: 278 SWVPSKLK--LSFLPRPKQLPPKYKCQLAVE--PSLIASMAY 165
S+ P KLK LSFLP+ K +PP Y +A+ P+++A++ +
Sbjct: 579 SFAPGKLKQWLSFLPQVKGVPPLYIMAVALYLLPNVLAAIMF 620
>At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2)
identical to GB:AF043519
Length = 246
Score = 29.9 bits (64), Expect = 1.0
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +1
Query: 142 EGRLFQVEYAIEAIK 186
EGRLFQVEYA +A+K
Sbjct: 19 EGRLFQVEYAFKAVK 33
>At4g37310.1 68417.m05283 cytochrome P450, putative
Length = 518
Score = 28.7 bits (61), Expect = 2.4
Identities = 14/39 (35%), Positives = 21/39 (53%)
Frame = -2
Query: 351 VLKSPIRPETAQPMCRSTSTIFSMVVGSIKVEVILSPTA 235
+LK P+ T + S +FS+ +GS +I SPTA
Sbjct: 46 LLKKPLLHRTLSHLSHSLGPVFSLRLGSRLAVIISSPTA 84
>At1g06770.2 68414.m00719 zinc finger (C3HC4-type RING finger)
family protein
Length = 307
Score = 28.3 bits (60), Expect = 3.1
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +1
Query: 391 FVYNERMSVESCAQALSNLTIQFGDTDDDSRTAMSP 498
F N+R S +SC +++SN + GD DS+ P
Sbjct: 54 FTQNKRQSKKSCKESISNKENKDGDEPWDSKMDWKP 89
>At1g06770.1 68414.m00720 zinc finger (C3HC4-type RING finger)
family protein
Length = 421
Score = 28.3 bits (60), Expect = 3.1
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +1
Query: 391 FVYNERMSVESCAQALSNLTIQFGDTDDDSRTAMSP 498
F N+R S +SC +++SN + GD DS+ P
Sbjct: 168 FTQNKRQSKKSCKESISNKENKDGDEPWDSKMDWKP 203
>At2g32810.1 68415.m04016 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase GI:7939617 from
[Lycopersicon esculentum]
Length = 887
Score = 27.9 bits (59), Expect = 4.1
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = +1
Query: 190 GSTANWHLYFGGSCFGR 240
GS N+++YFGG+ FGR
Sbjct: 296 GSFQNYYMYFGGTNFGR 312
>At5g63800.1 68418.m08007 glycosyl hydrolase family 35 protein
similar to beta-galactosidase GI:7939621 from
[Lycopersicon esculentum]; contains Pfam profile
PF01301: Glycosyl hydrolases family 35
Length = 718
Score = 27.1 bits (57), Expect = 7.2
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = +1
Query: 169 AIEAIKLGSTANWHLYFGGSCFGRGRKDNF 258
A+ K GS N+++Y GG+ FGR F
Sbjct: 285 ALFVAKNGSYINYYMYHGGTNFGRTSSSYF 314
>At5g45470.1 68418.m05586 expressed protein contains Pfam domain,
PF04578: Protein of unknown function, DUF594
Length = 866
Score = 27.1 bits (57), Expect = 7.2
Identities = 11/33 (33%), Positives = 16/33 (48%)
Frame = -1
Query: 178 LLWHIPLGTAFLQGLNVLTPRSYSERVKHFTIR 80
L+WHI + Q P Y E+ KH++ R
Sbjct: 665 LMWHIATELCYQQHEKETIPEGYDEQRKHYSNR 697
>At1g77410.1 68414.m09015 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase SP:P45582 from
[Asparagus officinalis]
Length = 815
Score = 26.6 bits (56), Expect = 9.5
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +1
Query: 169 AIEAIKLGSTANWHLYFGGSCFGR 240
A+ K GS N+++Y GG+ FGR
Sbjct: 278 ALFIAKNGSFVNYYMYHGGTNFGR 301
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,543,842
Number of Sequences: 28952
Number of extensions: 257285
Number of successful extensions: 672
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 644
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 670
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 898188928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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