BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0235 (812 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC365.08c |||Der1-like |Schizosaccharomyces pombe|chr 2|||Manual 69 1e-12 SPAC1687.17c |||Der1-like |Schizosaccharomyces pombe|chr 1|||Manual 60 4e-10 SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC su... 27 2.4 SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 27 3.2 SPBC3B9.15c |scp1||sterol regulatory element binding protein Scp... 26 5.5 SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 26 7.3 SPAC23C11.04c |pnk1||DNA kinase/phosphatase Pnk1|Schizosaccharom... 26 7.3 SPAC11G7.06c |mug132||S. pombe specific UPF0300 family protein 3... 26 7.3 >SPBC365.08c |||Der1-like |Schizosaccharomyces pombe|chr 2|||Manual Length = 224 Score = 68.5 bits (160), Expect = 1e-12 Identities = 46/155 (29%), Positives = 65/155 (41%), Gaps = 1/155 (0%) Frame = +1 Query: 256 IPPVTRAYTTACVVTTLAVQLDLVSPFQLYFNPNLILRKYQLWRXXXXXXXXXXXXXXXX 435 IPPVTR TT+ L+SP L + L++R+ Q W Sbjct: 16 IPPVTRYILLGTAATTILTLCQLLSPSMLVLHYPLVVRQKQ-WYRLFTNYLYAGTGFDFI 74 Query: 436 XXVIFTYRYCRMLEEGSFRSRTADFVVMFIFGGLLMIICAFFVNL-LFLGQAFTIMIVYV 612 + F Y+Y LE F +++ + LL+ + L L Q+ I Y Sbjct: 75 MNIYFFYQYSTYLENFVFARNAKKYIIYLVKVALLIDAFSLISGLGSALNQSLAAAIAYN 134 Query: 613 WSRRNVFVRMNFFGLMNFQAPYLPWVLLGFSVLLG 717 WS N F ++ F + Q YLP+VLLGFS L G Sbjct: 135 WSLFNSFSKIQFLFGFHVQGKYLPYVLLGFSFLTG 169 >SPAC1687.17c |||Der1-like |Schizosaccharomyces pombe|chr 1|||Manual Length = 168 Score = 60.1 bits (139), Expect = 4e-10 Identities = 31/113 (27%), Positives = 59/113 (52%), Gaps = 2/113 (1%) Frame = +1 Query: 448 FTYRYCRMLEEGSFRSRTADFVVMFIFGGLLMIICAFFVNLLFLGQAFTIMIVYVWSRRN 627 F R+ MLE S +T F+ + +++ ++F + F F+ ++Y+WS ++ Sbjct: 53 FLLRFMSMLERSSPPPQTQSFLKTVLIVWFSLLVTSYFSYMPFAASYFSFTMLYIWSWKH 112 Query: 628 VFVRMNFFGLMNFQAPYLPWVLLGFSVLLGMQFQLI*LDGN--RYIYFFLEEF 780 R++ GL + +APY+PWV++ L F L+ L ++YFF+ +F Sbjct: 113 PLYRISILGLFDVKAPYVPWVMVLLRWLRTGIFPLLDLISALIGHVYFFVTDF 165 >SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC subunit Psc3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 962 Score = 27.5 bits (58), Expect = 2.4 Identities = 16/54 (29%), Positives = 27/54 (50%), Gaps = 4/54 (7%) Frame = +1 Query: 202 LYYLDTMAYQTLLQEYM----LIPPVTRAYTTACVVTTLAVQLDLVSPFQLYFN 351 L Y + Y TLL+ + I P+T+A AC + ++ + +S F L+ N Sbjct: 487 LSYQSSFDYDTLLRIFQKNGNFITPITQALLNACEIDSIYQSWEDISNFVLFDN 540 >SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 435 Score = 27.1 bits (57), Expect = 3.2 Identities = 11/36 (30%), Positives = 18/36 (50%) Frame = +3 Query: 492 IENSRFCCHVHIWRLINDYMCFLC*SIVFRASVYNH 599 +E CCH++ L + CFLC + + + Y H Sbjct: 375 VERIDGCCHMNC--LCGTHFCFLCGAYLMEQNPYKH 408 >SPBC3B9.15c |scp1||sterol regulatory element binding protein Scp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1086 Score = 26.2 bits (55), Expect = 5.5 Identities = 14/32 (43%), Positives = 19/32 (59%) Frame = -3 Query: 756 VPIAIQLDQLKLHAEQN*ETKQNPGEVRCLEV 661 VP+ QLD LK+ +N +T + G RCL V Sbjct: 590 VPVHHQLDILKIAVSENYKTFASVGLDRCLVV 621 >SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 367 Score = 25.8 bits (54), Expect = 7.3 Identities = 14/43 (32%), Positives = 18/43 (41%) Frame = -2 Query: 733 STEIACRAELRNQAEPRGGKVPGSSLIQRSSFSRKHSCVTIHT 605 S + C+ + PR S +S SRK SCVT T Sbjct: 83 SEDYLCKKVSKGPESPRVNSFHNSYSRNQSPISRKSSCVTCST 125 >SPAC23C11.04c |pnk1||DNA kinase/phosphatase Pnk1|Schizosaccharomyces pombe|chr 1|||Manual Length = 421 Score = 25.8 bits (54), Expect = 7.3 Identities = 13/33 (39%), Positives = 21/33 (63%) Frame = -3 Query: 678 VRCLEVH*SKEVHSHENIPA*PYIHNHDCKRLP 580 +RC+ + S+E+ H N+ YIH H+ K+LP Sbjct: 349 IRCIHLQSSEELARHNNVFR--YIH-HNQKQLP 378 >SPAC11G7.06c |mug132||S. pombe specific UPF0300 family protein 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 25.8 bits (54), Expect = 7.3 Identities = 12/45 (26%), Positives = 21/45 (46%) Frame = +1 Query: 223 AYQTLLQEYMLIPPVTRAYTTACVVTTLAVQLDLVSPFQLYFNPN 357 A Q + + + T + AC +++ L+ P LYF+PN Sbjct: 198 AVQAHVMNQVRLGRATLLHARACYQKIGDIRMYLIDPHDLYFSPN 242 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,400,662 Number of Sequences: 5004 Number of extensions: 73428 Number of successful extensions: 180 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 173 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 179 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 396433620 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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