BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0235 (812 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AJ968562-1|CAI91546.1| 998|Apis mellifera protein ( Apis mellif... 25 0.83 DQ667188-1|ABG75740.1| 383|Apis mellifera histamine-gated chlor... 23 3.4 DQ667190-1|ABG75742.1| 509|Apis mellifera pH-sensitive chloride... 23 4.4 >AJ968562-1|CAI91546.1| 998|Apis mellifera protein ( Apis mellifera ORF for hypotheticalprotein. ). Length = 998 Score = 25.0 bits (52), Expect = 0.83 Identities = 13/31 (41%), Positives = 18/31 (58%) Frame = +3 Query: 675 LPPLGSAWFLSSARHAISVDLVGWQSVHLLF 767 LPP +A+FLS ARH + + V +LF Sbjct: 761 LPPHVAAYFLSRARHHDDLYSQSYAEVGVLF 791 >DQ667188-1|ABG75740.1| 383|Apis mellifera histamine-gated chloride channel protein. Length = 383 Score = 23.0 bits (47), Expect = 3.4 Identities = 11/32 (34%), Positives = 15/32 (46%) Frame = +1 Query: 256 IPPVTRAYTTACVVTTLAVQLDLVSPFQLYFN 351 + P T Y T C A+ +D VS F F+ Sbjct: 339 VRPATVQYDTCCQGRATAIYIDKVSRFFFPFS 370 >DQ667190-1|ABG75742.1| 509|Apis mellifera pH-sensitive chloride channel variant 1 protein. Length = 509 Score = 22.6 bits (46), Expect = 4.4 Identities = 16/69 (23%), Positives = 30/69 (43%), Gaps = 4/69 (5%) Frame = -1 Query: 575 NNRLTKKAHIIINKPPNMNMTTKSAVLDLKEPSSSILQYLYVNITLKKKLNPRFP-KNRN 399 NN T H+ + + + +L L P S L+Y + ++ +PR NR+ Sbjct: 120 NNHYTSHQHLRTHLRGTLTVNVSVLLLSLASPDESSLKYEVEFLLQQQWYDPRLRYSNRS 179 Query: 398 ---VVNSLH 381 +N++H Sbjct: 180 QYEFLNAIH 188 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 232,586 Number of Sequences: 438 Number of extensions: 5165 Number of successful extensions: 7 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 7 length of database: 146,343 effective HSP length: 57 effective length of database: 121,377 effective search space used: 25853301 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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