BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0186 (720 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 318 2e-87 At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 318 2e-87 At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 318 2e-87 At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 318 2e-87 At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 185 3e-47 At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 179 2e-45 At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 102 2e-22 At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 97 1e-20 At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 56 2e-08 At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 56 2e-08 At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 54 1e-07 At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 52 4e-07 At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 52 5e-07 At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 49 4e-06 At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 43 2e-04 At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 43 2e-04 At5g13650.2 68418.m01585 elongation factor family protein contai... 42 3e-04 At5g13650.1 68418.m01584 elongation factor family protein contai... 42 3e-04 At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 41 0.001 At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 40 0.001 At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 39 0.003 At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 37 0.015 At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 37 0.015 At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 34 0.082 At3g01360.1 68416.m00057 expressed protein contains Pfam profile... 31 1.0 At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.3 At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 30 1.3 At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.8 At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.8 At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 29 2.3 At5g35980.1 68418.m04333 protein kinase family protein contains ... 28 5.4 At3g43240.1 68416.m04564 ARID/BRIGHT DNA-binding domain-containi... 28 5.4 At1g26130.1 68414.m03190 haloacid dehalogenase-like hydrolase fa... 28 7.2 >At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha identical to SWISS-PROT:P13905 elongation factor 1-alpha (EF-1-alpha) [Arabidopsis thaliana] Length = 449 Score = 318 bits (782), Expect = 2e-87 Identities = 152/185 (82%), Positives = 164/185 (88%) Frame = +2 Query: 71 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 250 MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60 Query: 251 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 430 DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120 Query: 431 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 610 G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180 Query: 611 DWLQP 625 P Sbjct: 181 VGYNP 185 Score = 56.4 bits (130), Expect = 2e-08 Identities = 21/35 (60%), Positives = 28/35 (80%) Frame = +3 Query: 606 RKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 710 +K+GYNP + FVPISG+ GDNM+E ST + W+KG Sbjct: 179 KKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213 >At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 318 bits (782), Expect = 2e-87 Identities = 152/185 (82%), Positives = 164/185 (88%) Frame = +2 Query: 71 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 250 MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60 Query: 251 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 430 DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120 Query: 431 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 610 G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180 Query: 611 DWLQP 625 P Sbjct: 181 VGYNP 185 Score = 56.4 bits (130), Expect = 2e-08 Identities = 21/35 (60%), Positives = 28/35 (80%) Frame = +3 Query: 606 RKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 710 +K+GYNP + FVPISG+ GDNM+E ST + W+KG Sbjct: 179 KKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213 >At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 318 bits (782), Expect = 2e-87 Identities = 152/185 (82%), Positives = 164/185 (88%) Frame = +2 Query: 71 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 250 MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60 Query: 251 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 430 DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120 Query: 431 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 610 G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180 Query: 611 DWLQP 625 P Sbjct: 181 VGYNP 185 Score = 56.4 bits (130), Expect = 2e-08 Identities = 21/35 (60%), Positives = 28/35 (80%) Frame = +3 Query: 606 RKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 710 +K+GYNP + FVPISG+ GDNM+E ST + W+KG Sbjct: 179 KKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213 >At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 318 bits (782), Expect = 2e-87 Identities = 152/185 (82%), Positives = 164/185 (88%) Frame = +2 Query: 71 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 250 MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60 Query: 251 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 430 DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120 Query: 431 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 610 G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180 Query: 611 DWLQP 625 P Sbjct: 181 VGYNP 185 Score = 56.4 bits (130), Expect = 2e-08 Identities = 21/35 (60%), Positives = 28/35 (80%) Frame = +3 Query: 606 RKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 710 +K+GYNP + FVPISG+ GDNM+E ST + W+KG Sbjct: 179 KKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213 >At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / EF-1-alpha, putative contains similarity to SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha) [Aeropyrum pernix] Length = 667 Score = 185 bits (450), Expect = 3e-47 Identities = 84/175 (48%), Positives = 124/175 (70%), Gaps = 1/175 (0%) Frame = +2 Query: 86 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 265 + +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+ Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297 Query: 266 ERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEA 445 ERERGIT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEA Sbjct: 298 ERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEA 357 Query: 446 GISK-NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIK 607 G GQTREHA + GV+Q+IV +NKMD YS+ RF+ IK+ V S+++ Sbjct: 358 GFDNLKGQTREHARVLRGFGVEQVIVAINKMDIV--GYSKERFDLIKQHVGSFLQ 410 >At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein, putative similar to EF-1-alpha-related GTP-binding protein gi|1009232|gb|AAA79032 Length = 532 Score = 179 bits (435), Expect = 2e-45 Identities = 81/176 (46%), Positives = 123/176 (69%) Frame = +2 Query: 80 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 259 +K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157 Query: 260 KAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF 439 + ER +G T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEF Sbjct: 158 EEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEF 217 Query: 440 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIK 607 E G + GQTREH LA TLGV +LIV VNKMD +S+ R++EI++++ ++K Sbjct: 218 ETGYERGGQTREHVQLAKTLGVSKLIVVVNKMDDPTVNWSKERYDEIEQKMVPFLK 273 >At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) identical to SWISS-PROT:P17745 elongation factor Tu, chloroplast precursor (EF-Tu) [Arabidopsis thaliana] Length = 476 Score = 102 bits (245), Expect = 2e-22 Identities = 63/179 (35%), Positives = 93/179 (51%), Gaps = 1/179 (0%) Frame = +2 Query: 68 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV 247 K ++K H+NI IGHVD GK+T T L I +K+++ Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE--------------- 116 Query: 248 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 427 +D ER RGITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+ Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 176 Query: 428 TGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE-EIKKEVSSY 601 G QT+EH LLA +GV ++V +NK D + E E+++ +SSY Sbjct: 177 DGPMP-------QTKEHILLAKQVGVPDMVVFLNKEDQVDDAELLELVELEVRELLSSY 228 >At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu, putative similar to mitochondrial elongation factor Tu [Arabidopsis thaliana] gi|1149571|emb|CAA61511 Length = 454 Score = 97.1 bits (231), Expect = 1e-20 Identities = 62/177 (35%), Positives = 90/177 (50%) Frame = +2 Query: 77 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 256 + K H+N+ IGHVD GK+T T + K E GK +DK Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLTAAIT---------------KVLAEEGKAKAIAFDEIDK 107 Query: 257 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 436 E++RGITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G Sbjct: 108 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGP 167 Query: 437 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIK 607 QT+EH LLA +GV L+ +NK+D + P E +E+ S+ K Sbjct: 168 MP-------QTKEHILLARQVGVPSLVCFLNKVDVVDDPELLELVEMELRELLSFYK 217 >At2g45030.1 68415.m05606 mitochondrial elongation factor, putative similar to SP|P25039 Elongation factor G 1, mitochondrial precursor (mEF-G-1) {Saccharomyces cerevisiae}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03764: Elongation factor G domain IV, PF00679: Elongation factor G C-terminus Length = 754 Score = 56.4 bits (130), Expect = 2e-08 Identities = 41/128 (32%), Positives = 61/128 (47%) Frame = +2 Query: 95 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 274 NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116 Query: 275 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 454 +GITI A Y V IID PGH DF + D A+L++ + G I+ Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176 Query: 455 KNGQTREH 478 + Q R + Sbjct: 177 VDRQMRRY 184 >At1g45332.1 68414.m05195 mitochondrial elongation factor, putative similar to mitochondrial elongation factor GI:3917 from [Saccharomyces cerevisiae] Length = 754 Score = 56.4 bits (130), Expect = 2e-08 Identities = 41/128 (32%), Positives = 61/128 (47%) Frame = +2 Query: 95 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 274 NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116 Query: 275 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 454 +GITI A Y V IID PGH DF + D A+L++ + G I+ Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176 Query: 455 KNGQTREH 478 + Q R + Sbjct: 177 VDRQMRRY 184 >At5g08650.1 68418.m01029 GTP-binding protein LepA, putative Length = 681 Score = 53.6 bits (123), Expect = 1e-07 Identities = 54/184 (29%), Positives = 84/184 (45%), Gaps = 4/184 (2%) Frame = +2 Query: 95 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 274 N +I H+D GKST L+ G + R + K F LD + ERE Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDM-----------KEQF-----LDNMDLERE 131 Query: 275 RGITIDIAL----WKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFE 442 RGITI + + +E + + + +ID PGH DF + + + A+L+V A G E Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG-VE 190 Query: 443 AGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKDWLQ 622 A QT + LA + ++I +NK+D P +EP E++ +E+ I D + Sbjct: 191 A------QTLANVYLALENNL-EIIPVLNKIDL---PGAEP--EKVLREIEEVIGLDCSK 238 Query: 623 PSCC 634 C Sbjct: 239 AIFC 242 >At3g22980.1 68416.m02898 elongation factor Tu family protein similar to eukaryotic translation elongation factor 2 GB:NP_001952 [Homo sapiens] Length = 1015 Score = 52.0 bits (119), Expect = 4e-07 Identities = 36/113 (31%), Positives = 53/113 (46%) Frame = +2 Query: 95 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 274 NI ++ HVD GK+T HLI GG + GK F +D L E+ Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG--------GVLHPRLAGKLRF-----MDYLDEEQR 57 Query: 275 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 433 R IT+ + + Y + +ID+PGH DF + T +D A+++V A G Sbjct: 58 RAITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110 >At1g62750.1 68414.m07082 elongation factor Tu family protein similar to elongation factor G SP:P34811 [Glycine max (Soybean)] Length = 783 Score = 51.6 bits (118), Expect = 5e-07 Identities = 36/117 (30%), Positives = 56/117 (47%) Frame = +2 Query: 83 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 262 K + NI ++ H+D+GK+TTT ++Y G K E+ +G+ W ++ Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTGRNYK---------IGEVHEGTATMDW----ME 140 Query: 263 AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 433 E+ERGITI A K+ + IID PGH DF + D A+ + + G Sbjct: 141 QEQERGITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197 >At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-binding protein GUF1 - Saccharomyces cerevisiae, PIR:S50374 Length = 661 Score = 48.8 bits (111), Expect = 4e-06 Identities = 59/194 (30%), Positives = 81/194 (41%), Gaps = 9/194 (4%) Frame = +2 Query: 59 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKY 238 D K EK N +I H+D GKST L+ G I K G G +Y Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK-------------GHGQPQY 102 Query: 239 AWVLDKLKAERERGITIDIALWKF---------ETSKYYVTIIDAPGHRDFIKNMITGTS 391 LDKL +RERGIT+ E S Y + +ID PGH DF + S Sbjct: 103 ---LDKL--QRERGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDFSYEVSRSLS 157 Query: 392 QADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRF 571 A+L+V A G QT + LAF + ++ +NK+D P ++P Sbjct: 158 ACQGALLVVDAAQG-------VQAQTVANFYLAFEANL-TIVPVINKIDQ---PTADP-- 204 Query: 572 EEIKKEVSSYIKKD 613 E +K ++ S D Sbjct: 205 ERVKAQLKSMFDLD 218 >At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2 subunit 3, putative / eIF2S3, putative / eIF-2-gamma, putative similar to SP|Q09130 Eukaryotic translation initiation factor 2 gamma subunit (eIF-2- gamma) {Schizosaccharomyces pombe}; contains Pfam profile PF00009: Elongation factor Tu GTP binding domain; isoform predicted to contain a TG non-consensus acceptor splice site. Length = 471 Score = 43.2 bits (97), Expect = 2e-04 Identities = 49/197 (24%), Positives = 87/197 (44%), Gaps = 14/197 (7%) Frame = +2 Query: 65 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 235 P++ + INI IGHV GKST ++ G+ R + E+ ++G + K Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86 Query: 236 YAWVLDKLK---------AERERGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 382 Y DK + +E T D+ ++ T + +V+ +D PGH + M+ Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146 Query: 383 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE 562 G + D A+L++AA QT EH + +K +I+ NK+D + Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKIDLINEKAAT 200 Query: 563 PRFEEIKKEVSSYIKKD 613 + E I+K +++ +D Sbjct: 201 EQHEAIQKFITNTNAED 217 >At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2 subunit 3, putative / eIF2S3, putative / eIF-2-gamma, putative similar to SP|Q09130 Eukaryotic translation initiation factor 2 gamma subunit (eIF-2- gamma) {Schizosaccharomyces pombe}; contains Pfam profile PF00009: Elongation factor Tu GTP binding domain; isoform predicted to contain a TG non-consensus acceptor splice site. Length = 284 Score = 43.2 bits (97), Expect = 2e-04 Identities = 49/197 (24%), Positives = 87/197 (44%), Gaps = 14/197 (7%) Frame = +2 Query: 65 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 235 P++ + INI IGHV GKST ++ G+ R + E+ ++G + K Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86 Query: 236 YAWVLDKLK---------AERERGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 382 Y DK + +E T D+ ++ T + +V+ +D PGH + M+ Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146 Query: 383 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE 562 G + D A+L++AA QT EH + +K +I+ NK+D + Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKIDLINEKAAT 200 Query: 563 PRFEEIKKEVSSYIKKD 613 + E I+K +++ +D Sbjct: 201 EQHEAIQKFITNTNAED 217 >At5g13650.2 68418.m01585 elongation factor family protein contains Pfam profiles: PF00009 elongation factor Tu GTP binding domain,PF00679 elongation factor G C-terminus, PF03144 elongation factor Tu domain 2 Length = 676 Score = 42.3 bits (95), Expect = 3e-04 Identities = 46/159 (28%), Positives = 66/159 (41%) Frame = +2 Query: 95 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 274 NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE Sbjct: 85 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 129 Query: 275 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 454 RGITI V IID PGH DF + + D +L+V + G Sbjct: 130 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 185 Query: 455 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRF 571 QTR A G ++V VNK+D P + P F Sbjct: 186 ---QTRFVLKKALEFG-HAVVVVVNKIDR---PSARPEF 217 >At5g13650.1 68418.m01584 elongation factor family protein contains Pfam profiles: PF00009 elongation factor Tu GTP binding domain,PF00679 elongation factor G C-terminus, PF03144 elongation factor Tu domain 2 Length = 675 Score = 42.3 bits (95), Expect = 3e-04 Identities = 46/159 (28%), Positives = 66/159 (41%) Frame = +2 Query: 95 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 274 NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE Sbjct: 84 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 128 Query: 275 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 454 RGITI V IID PGH DF + + D +L+V + G Sbjct: 129 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 184 Query: 455 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRF 571 QTR A G ++V VNK+D P + P F Sbjct: 185 ---QTRFVLKKALEFG-HAVVVVVNKIDR---PSARPEF 216 >At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2 subunit 3, putative / eIF2S3, putative / eIF-2-gamma, putative Length = 465 Score = 40.7 bits (91), Expect = 0.001 Identities = 44/186 (23%), Positives = 82/186 (44%), Gaps = 8/186 (4%) Frame = +2 Query: 65 PKMGKEKTHINIVVIGHVDSGKST----TTG-HLIYKCGGIDKR-TIEKFEKEAQEMGKG 226 P++ + INI IGHV GKST +G H + +++ TI+ A+ Sbjct: 25 PEVISRQATINIGTIGHVAHGKSTIVKAVSGVHTVKFKSELERNITIKLGYANAKIYKCE 84 Query: 227 SFKYAWVLDKLKAERERGITIDIALWKFETSKY--YVTIIDAPGHRDFIKNMITGTSQAD 400 + +E + D++ + K +V+ +D PGH + M+ G + D Sbjct: 85 KCPRPVAYKSFGSGKEDNPSCDVSGHEKYKMKLLRHVSFVDCPGHDILMATMLNGAAIMD 144 Query: 401 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEI 580 A+LI+AA QT EH + +K +I+ NK+D + + + E+I Sbjct: 145 GALLIIAANE------TCPQPQTAEHLASVDMMHLKDIIIIQNKIDLIQENEAIKQHEDI 198 Query: 581 KKEVSS 598 ++ +++ Sbjct: 199 QRFITN 204 >At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2 subunit 3, putative / eIF2S3, putative / eIF-2-gamma, putative similar to gb|U37354 from S. pombe. ESTs gb|T41979, gb|N37284 and gb|N37529 come from this gene Length = 465 Score = 40.3 bits (90), Expect = 0.001 Identities = 28/103 (27%), Positives = 49/103 (47%), Gaps = 4/103 (3%) Frame = +2 Query: 308 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 475 FE SK +V+ +D PGH + M+ G + D A+L++AA QT E Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165 Query: 476 HALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 604 H + +K +I+ NK+D + + + E I+K + + + Sbjct: 166 HLAAVEIMQLKHIIILQNKIDLIQENVAINQHEAIQKFIMNTV 208 >At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, putative similar to ELONGATION FACTOR 2 GB:O14460 from [Schizosaccharomyces pombe] Length = 843 Score = 39.1 bits (87), Expect = 0.003 Identities = 30/112 (26%), Positives = 51/112 (45%), Gaps = 4/112 (3%) Frame = +2 Query: 95 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRT---IEKFEKEAQEMGKG-SFKYAWVLDKLK 262 N+ VI HVD GKST T L+ G I + + + A E +G + K + + Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGISLYYE 80 Query: 263 AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 418 E ++ + ++Y + +ID+PGH DF + D A+++V Sbjct: 81 MTDE---SLKSFTGARDGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129 >At1g06220.2 68414.m00656 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 36.7 bits (81), Expect = 0.015 Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%) Frame = +2 Query: 95 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 274 N+ ++GH+ GK+ L+ + + K EK KY D E+E Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187 Query: 275 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 433 R I+I + L + Y I+D PGH +F M AD AVLIV A G Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245 >At1g06220.1 68414.m00655 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 36.7 bits (81), Expect = 0.015 Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%) Frame = +2 Query: 95 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 274 N+ ++GH+ GK+ L+ + + K EK KY D E+E Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187 Query: 275 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 433 R I+I + L + Y I+D PGH +F M AD AVLIV A G Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245 >At4g11160.1 68417.m01808 translation initiation factor IF-2, mitochondrial, putative similar to SP|P46198|IF2M_BOVIN Translation initiation factor IF-2, mitochondrial precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus} Length = 743 Score = 34.3 bits (75), Expect = 0.082 Identities = 28/90 (31%), Positives = 43/90 (47%) Frame = +2 Query: 329 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 508 +T +D PGH F + G + D VL+VAA G QT E A + V Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDGVMP-------QTLEAIAHARSANV- 321 Query: 509 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSS 598 ++V +NK D P + P E++K +++S Sbjct: 322 PVVVAINKCDK---PGANP--EKVKYQLTS 346 >At3g01360.1 68416.m00057 expressed protein contains Pfam profile PF04819: Family of unknown function (DUF716) (Plant viral-response family) Length = 319 Score = 30.7 bits (66), Expect = 1.0 Identities = 27/87 (31%), Positives = 38/87 (43%), Gaps = 2/87 (2%) Frame = +2 Query: 338 IDAPGHRDFIKNMITGTSQADCAV-LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 514 I GH D+ + T Q +C + L+V TG F +KNG R+ LG + Sbjct: 226 IKCKGHGDYHRAKAIATLQFNCHLALMVVVATGLFSVIANKNGYLRQDHSKYRPLGAELE 285 Query: 515 IVGVNKMDSTEPPYSEPRFE-EIKKEV 592 + +DS E E R E + KEV Sbjct: 286 NLSTFTLDSDEE--DEVREESNVAKEV 310 >At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2 family protein / eIF-2 family protein similar to SP|O60841 Translation initiation factor IF-2 {Homo sapiens}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03144: Elongation factor Tu domain 2 Length = 1201 Score = 30.3 bits (65), Expect = 1.3 Identities = 21/68 (30%), Positives = 34/68 (50%) Frame = +2 Query: 335 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 514 +ID PGH F G+S D A+L+V + + G+ QT E +L + + Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIKHGLEP--QTIE-SLNLLRMRNTEF 756 Query: 515 IVGVNKMD 538 I+ +NK+D Sbjct: 757 IIALNKVD 764 >At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2 family protein / eIF-2 family protein similar to SP|O60841 Translation initiation factor IF-2 {Homo sapiens}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03144: Elongation factor Tu domain 2 Length = 1088 Score = 30.3 bits (65), Expect = 1.3 Identities = 22/70 (31%), Positives = 33/70 (47%) Frame = +2 Query: 329 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 508 + +ID PGH F G++ D A+L+V + G+ QT E L VK Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV-----DIMRGLEP--QTIESLNLLRRRNVK 610 Query: 509 QLIVGVNKMD 538 I+ +NK+D Sbjct: 611 -FIIALNKVD 619 >At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2 family protein / eIF-2 family protein similar to SP|O60841 Translation initiation factor IF-2 {Homo sapiens}; contains Pfam profile PF00009: Elongation factor Tu GTP binding domain Length = 630 Score = 29.9 bits (64), Expect = 1.8 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = +2 Query: 335 IIDAPGHRDFIKNMITGTSQADCAVLIV 418 +ID PGH F G+S D A+L+V Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140 >At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2 family protein / eIF-2 family protein similar to IF2 protein [Drosophila melanogaster] GI:7108770; contains Pfam profile PF03144: Elongation factor Tu domain 2 Length = 1294 Score = 29.9 bits (64), Expect = 1.8 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = +2 Query: 335 IIDAPGHRDFIKNMITGTSQADCAVLIV 418 +ID PGH F G+S D A+L+V Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799 >At1g17220.1 68414.m02098 translation initiation factor IF-2, chloroplast, putative similar to SP|P57997|IF2C_PHAVU Translation initiation factor IF-2, chloroplast precursor (PvIF2cp) {Phaseolus vulgaris} Length = 1026 Score = 29.5 bits (63), Expect = 2.3 Identities = 26/99 (26%), Positives = 38/99 (38%), Gaps = 4/99 (4%) Frame = +2 Query: 254 KLKAERERGITIDIALWKFET----SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 421 K+ A GIT I +K +D PGH F G D A+++VA Sbjct: 525 KVAASEAGGITQGIGAYKVSVPVDGKLQSCVFLDTPGHEAFGAMRARGARVTDIAIIVVA 584 Query: 422 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 538 A G QT E A+ +++ +NK+D Sbjct: 585 ADDG-------IRPQTNE-AIAHAKAAAVPIVIAINKID 615 >At5g35980.1 68418.m04333 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 956 Score = 28.3 bits (60), Expect = 5.4 Identities = 15/52 (28%), Positives = 25/52 (48%), Gaps = 2/52 (3%) Frame = +3 Query: 561 SPDLRKSRRKYPHTS--RKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 710 SPD R+ +YPH + +G +P+A F P+ + P+T + G Sbjct: 587 SPDARRRVMQYPHGNGPNGLGTSPSAGNFAPLPLGTSPSQFTPNTNNQFLAG 638 >At3g43240.1 68416.m04564 ARID/BRIGHT DNA-binding domain-containing protein contains Pfam profile PF01388: ARID/BRIGHT DNA binding domain Length = 747 Score = 28.3 bits (60), Expect = 5.4 Identities = 13/34 (38%), Positives = 16/34 (47%) Frame = +2 Query: 611 DWLQPSCCRFRAHFWMARRQHVGAFNQNALVQGM 712 DW+ C AHF RR +GAF A G+ Sbjct: 687 DWVNCGSCGEWAHFGCDRRPGLGAFKDYAKTDGL 720 >At1g26130.1 68414.m03190 haloacid dehalogenase-like hydrolase family protein similar to Potential phospholipid-transporting ATPase (EC 3.6.3.1) from Homo sapiens [SP|Q9Y2Q0, SP|O43520], Mus musculus [SP|P98200, SP|P70704], {Bos taurus} SP|Q29449; contains InterPro accession IPR005834: Haloacid dehalogenase-like hydrolase Length = 1184 Score = 27.9 bits (59), Expect = 7.2 Identities = 15/53 (28%), Positives = 24/53 (45%) Frame = +2 Query: 440 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSS 598 E+G +TR+H G++ LI+ ++D E R E K VS+ Sbjct: 629 ESGRKYEKETRDHVNEYADAGLRTLILAYRELDENEYEVFTERISEAKNSVSA 681 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,520,780 Number of Sequences: 28952 Number of extensions: 346758 Number of successful extensions: 1077 Number of sequences better than 10.0: 33 Number of HSP's better than 10.0 without gapping: 1024 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1062 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1565336320 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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