BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0158 (614 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 295 2e-81 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 163 1e-41 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 150 1e-37 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 120 1e-28 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 91 1e-19 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 89 6e-19 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 82 7e-17 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 42 7e-05 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 34 0.014 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 34 0.014 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.13 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.31 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 28 0.93 SPCC622.02 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 27 2.2 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 2.8 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.8 SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 26 5.0 SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 25 8.7 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 295 bits (725), Expect = 2e-81 Identities = 140/172 (81%), Positives = 147/172 (85%) Frame = +2 Query: 92 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 271 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 272 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 451 RGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121 Query: 452 IMFETFNTPAMYVAIQAVLSLYASRSYHRYRAGLPRRCLPTPCPIYQGYPTP 607 I+FETFN PA YVAIQAVLSLYAS T PIY+GY P Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHT-VPIYEGYALP 172 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 163 bits (397), Expect = 1e-41 Identities = 80/170 (47%), Positives = 109/170 (64%), Gaps = 4/170 (2%) Frame = +2 Query: 110 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 289 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG+L + Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKI 71 Query: 290 KYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPKANREKMTQIMFET 466 + PIE GI+ NW DME+IW + + + +L PEEHP+LLTE PL N+EK+ + +ET Sbjct: 72 QRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYET 131 Query: 467 FNTPAMYVAIQAVLSLYASRSYHRYRAGLPRRC---LPTPCPIYQGYPTP 607 N PA+ ++Q VL+LYAS G+ C L PIY G+ P Sbjct: 132 LNVPALSFSLQPVLALYASAR----TTGIVLECGDGLTHSVPIYDGFSIP 177 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 150 bits (364), Expect = 1e-37 Identities = 71/143 (49%), Positives = 101/143 (70%), Gaps = 2/143 (1%) Frame = +2 Query: 98 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSK 271 E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61 Query: 272 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 451 R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + Sbjct: 62 RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCE 121 Query: 452 IMFETFNTPAMYVAIQAVLSLYA 520 MFE + +YVAIQAVLSLYA Sbjct: 122 TMFERYGFGGVYVAIQAVLSLYA 144 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 120 bits (290), Expect = 1e-28 Identities = 66/162 (40%), Positives = 91/162 (56%), Gaps = 24/162 (14%) Frame = +2 Query: 110 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 238 +++DNG+G K G+AG+DAP VFP+++ R G G M K Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66 Query: 239 ------DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPV 397 D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126 Query: 398 LLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYAS 523 LLTE PLNP NRE +IMFE+FN +Y+A+QAVL+L AS Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAAS 168 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 91.1 bits (216), Expect = 1e-19 Identities = 51/143 (35%), Positives = 77/143 (53%) Frame = +2 Query: 95 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 274 +EV+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61 Query: 275 GILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 454 G + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + Sbjct: 62 G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120 Query: 455 MFETFNTPAMYVAIQAVLSLYAS 523 MFE+ PA Y+A Q + +AS Sbjct: 121 MFESLRCPATYLAKQETCAAFAS 143 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 88.6 bits (210), Expect = 6e-19 Identities = 45/137 (32%), Positives = 79/137 (57%), Gaps = 1/137 (0%) Frame = +2 Query: 110 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 289 LV+DNGS +AG+ G+ P+ VF ++V R R + + + + VG++ + G ++ Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK----LSRTSTLVGNDTLIEVGSRSI 82 Query: 290 -KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFET 466 + P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE Sbjct: 83 ARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFEL 141 Query: 467 FNTPAMYVAIQAVLSLY 517 +N P++ I + S Y Sbjct: 142 YNAPSVAYGIDGLFSFY 158 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 81.8 bits (193), Expect = 7e-17 Identities = 44/145 (30%), Positives = 79/145 (54%), Gaps = 2/145 (1%) Frame = +2 Query: 95 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 274 EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61 Query: 275 GI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 448 I + +K +GI+ +W+ W +L+V P E+ +++TE NP++ R+++ Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121 Query: 449 QIMFETFNTPAMYVAIQAVLSLYAS 523 + FE + PA Y+ QAV +A+ Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFAN 146 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 41.9 bits (94), Expect = 7e-05 Identities = 25/84 (29%), Positives = 38/84 (45%), Gaps = 4/84 (4%) Frame = +2 Query: 296 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 463 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 464 TFNTPAMYVAIQAVLSLYASRSYH 535 P +A + ++ LYA H Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILH 195 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 34.3 bits (75), Expect = 0.014 Identities = 19/64 (29%), Positives = 33/64 (51%), Gaps = 2/64 (3%) Frame = +2 Query: 356 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASRS 529 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + + A+ L Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQHLNIQAV 1458 Query: 530 YHRY 541 H Y Sbjct: 1459 NHAY 1462 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 34.3 bits (75), Expect = 0.014 Identities = 31/128 (24%), Positives = 55/128 (42%), Gaps = 4/128 (3%) Frame = +2 Query: 110 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 289 +V+DNG+ KAGFAG P+ + R + G + ++G+E + TL Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57 Query: 290 KYPIEH--GIVTNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 457 ++ H G + +W +W N + P ++ +LLT+ + Q++ Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117 Query: 458 FETFNTPA 481 FE F A Sbjct: 118 FEEFQFDA 125 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 31.1 bits (67), Expect = 0.13 Identities = 16/48 (33%), Positives = 26/48 (54%) Frame = +2 Query: 155 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 298 G+ PRA F ++ P H G+++ M KD G+E S +G + + P Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.9 bits (64), Expect = 0.31 Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%) Frame = -3 Query: 252 PT*ESFCPI-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 91 PT S P+ PT+ P P N S P PL+TTN TS+S Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 28.3 bits (60), Expect = 0.93 Identities = 13/30 (43%), Positives = 16/30 (53%) Frame = -1 Query: 263 VPHLLHKSPSVPYRPSRPDGGAFPRSRGTR 174 + L+ K+PS PY SRP A S TR Sbjct: 306 IDQLISKAPSYPYSSSRPSASASLASSPTR 335 >SPCC622.02 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual Length = 127 Score = 27.1 bits (57), Expect = 2.2 Identities = 23/60 (38%), Positives = 28/60 (46%) Frame = -2 Query: 292 FEGQDTSFALCLISYIRVLLSHTDHHALMAGPSHDRGEHGARSIISCETGLAHTGAIVYY 113 F G +F CLI++IR +SH D EHG II C T L G I+YY Sbjct: 47 FAGLGIAFIYCLIAFIRE-MSHPSSR-------KDTMEHGL-PIILCST-LMLVGNILYY 96 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.6 bits (56), Expect = 2.8 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%) Frame = -3 Query: 255 SPT*ESFC--PIPTITP*WRGLPTIEGNTARGASSPAKPAL 139 S T +SF P+PT P LPT NT + P+ PAL Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 3.8 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +2 Query: 398 LLTEAPLNPKANREKMTQIMFETFN 472 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|Schizosaccharomyces pombe|chr 2|||Manual Length = 2337 Score = 25.8 bits (54), Expect = 5.0 Identities = 10/27 (37%), Positives = 17/27 (62%) Frame = -2 Query: 292 FEGQDTSFALCLISYIRVLLSHTDHHA 212 +EG+DT+ +Y+R++L TD A Sbjct: 74 YEGEDTTRITRFANYLRIILPGTDQKA 100 >SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 564 Score = 25.0 bits (52), Expect = 8.7 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +2 Query: 215 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 301 +M+G +Y+G AQ KR +LT + I Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,704,990 Number of Sequences: 5004 Number of extensions: 58523 Number of successful extensions: 173 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 150 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 163 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 269634532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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