Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ceN-0134
(725 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1 pro... 33 0.15
At5g26200.1 68418.m03118 mitochondrial substrate carrier family ... 32 0.45
At3g49990.1 68416.m05466 expressed protein 31 0.59
At5g09530.1 68418.m01103 hydroxyproline-rich glycoprotein family... 31 1.0
At5g63550.1 68418.m07976 expressed protein 30 1.4
At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /... 30 1.4
At1g62480.1 68414.m07050 vacuolar calcium-binding protein-relate... 29 2.4
At3g10660.1 68416.m01282 calcium-dependent protein kinase isofor... 27 2.8
At5g27650.1 68418.m03313 PWWP domain-containing protein hypothet... 29 3.1
At2g45800.1 68415.m05696 LIM domain-containing protein similar t... 29 4.1
At2g02170.1 68415.m00153 remorin family protein contains Pfam do... 29 4.1
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 28 5.5
At1g03100.1 68414.m00285 pentatricopeptide (PPR) repeat-containi... 28 5.5
At5g19250.1 68418.m02292 expressed protein 28 7.2
At1g07830.1 68414.m00849 ribosomal protein L29 family protein si... 28 7.2
At4g25610.1 68417.m03689 zinc finger (C2H2 type) family protein ... 27 9.6
At4g20260.2 68417.m02960 DREPP plasma membrane polypeptide famil... 27 9.6
At4g20260.1 68417.m02959 DREPP plasma membrane polypeptide famil... 27 9.6
At3g03810.1 68416.m00391 expressed protein contains Pfam PF03138... 27 9.6
At2g22140.1 68415.m02630 expressed protein ; expression supporte... 27 9.6
>At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1
protein GI:1279562 from [Medicago sativa]
Length = 557
Score = 33.5 bits (73), Expect = 0.15
Identities = 15/51 (29%), Positives = 28/51 (54%)
Frame = +2
Query: 407 PDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSA 559
P A AK+ +K ++ ++ +DS +E + +K KP+A D+ S D +
Sbjct: 141 PAAAAKNGSVKAKKESSSEDDSSSEDEPAKKPAAKIAKPAAKDSSSSDDDS 191
Score = 29.1 bits (62), Expect = 3.1
Identities = 20/64 (31%), Positives = 29/64 (45%)
Frame = +2
Query: 398 SEIPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPQH 577
S D AK K+ +PAA+ S + E S++EKP+ A +A AA
Sbjct: 162 SSSEDEPAKKPAAKIAKPAAKDSSSSDDDSD---EDSEDEKPATKKAAPAAAKAASSSDS 218
Query: 578 GEED 589
+ED
Sbjct: 219 SDED 222
Score = 27.5 bits (58), Expect = 9.6
Identities = 19/81 (23%), Positives = 35/81 (43%)
Frame = +2
Query: 299 AKKDDIAPEDSDIAXXXXXXXXXXXXXXXXAKSSEIPDAEAKSADIKVEEPAAQPEDSKT 478
+ DD + EDS+ + S D++ +S D E+PA + D+K
Sbjct: 185 SSSDDDSDEDSEDEKPATKKAAPAAAKAASSSDSSDEDSDEESED---EKPAQKKADTKA 241
Query: 479 EVQATVAEISKEEKPSATDAE 541
+++ E S+ E+ + D E
Sbjct: 242 SKKSSSDESSESEEDESEDEE 262
>At5g26200.1 68418.m03118 mitochondrial substrate carrier family
protein contains Pfam profile: PF00153 mitochondrial
carrier protein
Length = 342
Score = 31.9 bits (69), Expect = 0.45
Identities = 17/35 (48%), Positives = 24/35 (68%)
Frame = -3
Query: 213 LTESSILGATSRIG*AGTTSFSSATGIAKLTAAKA 109
+T+SS+ AT R+G + TTS + A G A LT+A A
Sbjct: 112 ITKSSVGQATVRLGLSDTTSLAVANGAAGLTSAVA 146
>At3g49990.1 68416.m05466 expressed protein
Length = 502
Score = 31.5 bits (68), Expect = 0.59
Identities = 17/46 (36%), Positives = 25/46 (54%)
Frame = +2
Query: 404 IPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAE 541
+P A+ D+K E P A+P KT Q + E K+E+ +A AE
Sbjct: 422 LPGRRAEQTDVKAEIPKAEPIKRKTHGQESKEE--KKERKNAVKAE 465
>At5g09530.1 68418.m01103 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 370
Score = 30.7 bits (66), Expect = 1.0
Identities = 27/72 (37%), Positives = 38/72 (52%), Gaps = 6/72 (8%)
Frame = +3
Query: 486 KLPSL-KFQKKKNLVLLMQKVLLTQLPSFPNM--VKKIDLA--PTV-KSDAAAVPEIKTP 647
KLP + K + K V +QK L ++P P M ++K +L P V K +A VPEI+ P
Sbjct: 201 KLPEVPKLEAPK--VPEIQKPELPKMPELPKMPEIQKPELPKLPEVPKLEAPKVPEIQKP 258
Query: 648 EAADAPKLADNP 683
E P+L P
Sbjct: 259 ELPKMPELPKMP 270
Score = 30.3 bits (65), Expect = 1.4
Identities = 21/71 (29%), Positives = 35/71 (49%)
Frame = +3
Query: 480 KYKLPSLKFQKKKNLVLLMQKVLLTQLPSFPNMVKKIDLAPTVKSDAAAVPEIKTPEAAD 659
K +LP + +K L M ++ +LP FP + K DL ++ VP++ E +
Sbjct: 130 KPELPKVPEIQKPELPK-MPEIPKPELPKFPE-IPKPDLPKFPENSKPEVPKLMETEKPE 187
Query: 660 APKLADNPVNE 692
APK+ + P E
Sbjct: 188 APKVPEIPKPE 198
Score = 27.5 bits (58), Expect = 9.6
Identities = 22/70 (31%), Positives = 31/70 (44%), Gaps = 2/70 (2%)
Frame = +3
Query: 480 KYKLPSLKFQKKKNLVLLMQKVLLTQLPSFPNMVK-KIDLAPTV-KSDAAAVPEIKTPEA 653
K LP K + LM+ + P P + K ++ P V K +A VPEI+ PE
Sbjct: 163 KPDLPKFPENSKPEVPKLMETEK-PEAPKVPEIPKPELPKLPEVPKLEAPKVPEIQKPEL 221
Query: 654 ADAPKLADNP 683
P+L P
Sbjct: 222 PKMPELPKMP 231
>At5g63550.1 68418.m07976 expressed protein
Length = 530
Score = 30.3 bits (65), Expect = 1.4
Identities = 13/54 (24%), Positives = 30/54 (55%)
Frame = +2
Query: 401 EIPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAA 562
E D + + +++VE+P+ + SK V+ + K+++PSA + S + ++
Sbjct: 320 EKTDTDDEKDEVEVEKPSKKKSSSKKTVEESSGSKGKDKQPSAKGSARSGEKSS 373
>At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to SEC14-like protein 2
(Alpha-tocopherol associated protein) (TAP) (bTAP)
(Fragment) (SP:P58875) {Bos taurus}
Length = 683
Score = 30.3 bits (65), Expect = 1.4
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = +2
Query: 407 PDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSA 529
P E K + K EE + E+ KTE ++ AE +EEK +A
Sbjct: 123 PVKEEKVEEKKTEETEEKKEEVKTEEKSLEAETKEEEKSAA 163
Score = 27.9 bits (59), Expect = 7.2
Identities = 24/76 (31%), Positives = 35/76 (46%), Gaps = 8/76 (10%)
Frame = +2
Query: 392 KSSEIPDAEAK-SADIKVEEPAAQP---EDSKTEVQAT----VAEISKEEKPSATDAEGS 547
K E+ E A+ K EE +A P E K E+ A VAE KEE P A +
Sbjct: 140 KKEEVKTEEKSLEAETKEEEKSAAPATVETKKEEILAAPAPIVAETKKEETPVAPAPVET 199
Query: 548 ADSAAIIPQHGEEDRL 595
+A ++ + +E+ L
Sbjct: 200 KPAAPVVAETKKEEIL 215
>At1g62480.1 68414.m07050 vacuolar calcium-binding protein-related
contains weak similarity to vacuolar calcium binding
protein [Raphanus sativus] gi|9049359|dbj|BAA99394
Length = 152
Score = 29.5 bits (63), Expect = 2.4
Identities = 21/83 (25%), Positives = 38/83 (45%), Gaps = 2/83 (2%)
Frame = +2
Query: 278 EVPAIPEAKKDDIAPEDSDIAXXXXXXXXXXXXXXXXAKSSEIPDAEAKSADIKV-EEPA 454
E P + E KKD+ AP ++ + K E+ + + + A + V EE
Sbjct: 73 ETPEVVEIKKDEEAPVETPVV---VEDESKTEEVVEAKKEEEVEEKKTEEAPVVVEEEKK 129
Query: 455 AQPEDSKTEVQATV-AEISKEEK 520
+ E+ K V+A+V A + K ++
Sbjct: 130 PEAEEEKPAVEASVTAPVEKADE 152
>At3g10660.1 68416.m01282 calcium-dependent protein kinase isoform 2
(CPK2) identical to calcium-dependent protein kinase
isoform 2 [Arabidopsis thaliana] gi|9837343|gb|AAG00535;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 646
Score = 27.1 bits (57), Expect(2) = 2.8
Identities = 17/48 (35%), Positives = 29/48 (60%), Gaps = 1/48 (2%)
Frame = +2
Query: 401 EIPDAEAKSADIKVEEPAAQPED-SKTEVQATVAEISKEEKPSATDAE 541
E+P E+K ++ V+ +A+PE S+++ + T E + E KP T AE
Sbjct: 109 EVPPEESKR-EVVVQPESAKPETKSESKPETTKPETTSETKPE-TKAE 154
Score = 20.6 bits (41), Expect(2) = 2.8
Identities = 6/14 (42%), Positives = 10/14 (71%)
Frame = +2
Query: 290 IPEAKKDDIAPEDS 331
+PE K ++ PE+S
Sbjct: 102 VPEESKQEVPPEES 115
>At5g27650.1 68418.m03313 PWWP domain-containing protein
hypothetical protein F22F7.12 - Arabidopsis thaliana,
EMBL:AC009606
Length = 1072
Score = 29.1 bits (62), Expect = 3.1
Identities = 14/71 (19%), Positives = 34/71 (47%)
Frame = +2
Query: 389 AKSSEIPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAII 568
A + + P + KS + ++ ++ E A+ + +E+ + E + AA++
Sbjct: 461 APTLQTPMKDEKSGIVSMDFASSNTAIPGKEFSASKPSLDEEKGLAEKSKERMEERAAVL 520
Query: 569 PQHGEEDRLGS 601
P+HG+ + + S
Sbjct: 521 PEHGKSEAMAS 531
>At2g45800.1 68415.m05696 LIM domain-containing protein similar to
PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain
protein PLIM1 [Nicotiana tabacum] GI:5932418; contains
Pfam profile PF00412: LIM domain
Length = 226
Score = 28.7 bits (61), Expect = 4.1
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = +2
Query: 275 DEVPAIPEAKKDDIAPE 325
D+ +IPEAK+DD APE
Sbjct: 197 DDTASIPEAKEDDAAPE 213
>At2g02170.1 68415.m00153 remorin family protein contains Pfam
domain, PF03763: Remorin, C-terminal region
Length = 486
Score = 28.7 bits (61), Expect = 4.1
Identities = 19/63 (30%), Positives = 31/63 (49%)
Frame = +2
Query: 410 DAEAKSADIKVEEPAAQPEDSKTEVQATVAEISKEEKPSATDAEGSADSAAIIPQHGEED 589
+AE K ++KVE + +D + AT+ E EEK +A +A+ +A Q +
Sbjct: 411 EAEMKKTEVKVERIKGRAQDRLMKKLATI-ERKAEEKRAAAEAKKDHQAAKTEKQAEQIR 469
Query: 590 RLG 598
R G
Sbjct: 470 RTG 472
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 28.3 bits (60), Expect = 5.5
Identities = 21/77 (27%), Positives = 36/77 (46%), Gaps = 9/77 (11%)
Frame = +2
Query: 389 AKSSEIPDAEAKSADIKVEEPAAQPE---------DSKTEVQATVAEISKEEKPSATDAE 541
A E P AE ++ VEE AAQPE + + E + A+ K++K + +
Sbjct: 308 AALGETPAAERPASSTPVEEKAAQPEPVAPVENAGEKEGEEETAAAKKKKKKKEKEKEKK 367
Query: 542 GSADSAAIIPQHGEEDR 592
+A +AA +E++
Sbjct: 368 AAAAAAATSSVEVKEEK 384
>At1g03100.1 68414.m00285 pentatricopeptide (PPR) repeat-containing
protein low similarity to CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 793
Score = 28.3 bits (60), Expect = 5.5
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +3
Query: 411 MLKQNLLILKWKNQLLSLKIQKLKYKLPSLKFQKKKNL 524
M K+N+ + K+K ++L LK K YK + K Q + L
Sbjct: 740 MEKKNMFVDKYKYRMLFLKYHKTAYKGKAPKVQSESQL 777
>At5g19250.1 68418.m02292 expressed protein
Length = 196
Score = 27.9 bits (59), Expect = 7.2
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +3
Query: 561 PSFPNMVKKIDLAPTVKSDAAAVP 632
P+ PN++ K L PTV D A +P
Sbjct: 88 PNLPNLLSKCRLNPTVTRDGAILP 111
>At1g07830.1 68414.m00849 ribosomal protein L29 family protein
similar to GB:CAA83057 from [Saccharomyces cerevisiae]
Length = 144
Score = 27.9 bits (59), Expect = 7.2
Identities = 23/79 (29%), Positives = 36/79 (45%), Gaps = 4/79 (5%)
Frame = +3
Query: 441 WKNQLLSLK----IQKLKYKLPSLKFQKKKNLVLLMQKVLLTQLPSFPNMVKKIDLAPTV 608
WK L LK +QKL Y L K+KN+++ +++L Q FPN + P V
Sbjct: 60 WKASELRLKSWDDLQKLWYVL-----LKEKNMLMTQRQMLQAQNMQFPNP----ERIPKV 110
Query: 609 KSDAAAVPEIKTPEAADAP 665
+ + + T A + P
Sbjct: 111 RRSMCRIKHVLTERAIEEP 129
>At4g25610.1 68417.m03689 zinc finger (C2H2 type) family protein
contains Pfam profile: PF00096 zinc finger, C2H2 type
Length = 586
Score = 27.5 bits (58), Expect = 9.6
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -1
Query: 377 FLRFSLQERFPVSRYRSLLGLYRPFWPLV 291
FL F E PV ++ L L RP WPL+
Sbjct: 25 FLSFPRPENTPVQLFQLLHTLERPGWPLL 53
>At4g20260.2 68417.m02960 DREPP plasma membrane polypeptide family
protein contains Pfam profile: PF05558 DREPP plasma
membrane polypeptide
Length = 225
Score = 27.5 bits (58), Expect = 9.6
Identities = 16/45 (35%), Positives = 21/45 (46%), Gaps = 1/45 (2%)
Frame = +2
Query: 401 EIPDAEAKSADIKVEEPAAQPEDSKTE-VQATVAEISKEEKPSAT 532
E+ E ++K EEPA E +KTE EI +E K T
Sbjct: 133 EVKTKEIPVEEVKAEEPAKTEEPAKTEGTSGEKEEIVEETKKGET 177
>At4g20260.1 68417.m02959 DREPP plasma membrane polypeptide family
protein contains Pfam profile: PF05558 DREPP plasma
membrane polypeptide
Length = 225
Score = 27.5 bits (58), Expect = 9.6
Identities = 16/45 (35%), Positives = 21/45 (46%), Gaps = 1/45 (2%)
Frame = +2
Query: 401 EIPDAEAKSADIKVEEPAAQPEDSKTE-VQATVAEISKEEKPSAT 532
E+ E ++K EEPA E +KTE EI +E K T
Sbjct: 133 EVKTKEIPVEEVKAEEPAKTEEPAKTEGTSGEKEEIVEETKKGET 177
>At3g03810.1 68416.m00391 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'auxin-independent growth promoter protein -related'
based on similarity to axi 1 protein (GB:X80301)
(GI:559920) from [Nicotiana tabacum], which, due to
scienitific fraud was retracted. Retraction in: Schell
J. EMBO J 1999 May 17;18(10):2908. PMID:10400497.
Length = 656
Score = 27.5 bits (58), Expect = 9.6
Identities = 16/55 (29%), Positives = 25/55 (45%), Gaps = 2/55 (3%)
Frame = +3
Query: 537 QKVLLTQLPSFPNMVKKIDLAPTVKSDAAAVPEIKTPEAA--DAPKLADNPVNED 695
Q+VL+ FPN+V + L T + PE PE P+ +D + E+
Sbjct: 356 QRVLIPLRAMFPNLVDRTSLCSTEELSELVGPETPLPENTYKMPPRKSDKQLKEE 410
>At2g22140.1 68415.m02630 expressed protein ; expression supported
by MPSS
Length = 506
Score = 27.5 bits (58), Expect = 9.6
Identities = 13/49 (26%), Positives = 27/49 (55%), Gaps = 1/49 (2%)
Frame = +3
Query: 552 TQLPSFPNMVKKIDLAPTVKSDAAAVPEIKTPEAADAP-KLADNPVNED 695
T LPS +K ++ + SD+ P+ + PE++ P + + P+++D
Sbjct: 15 TPLPSLSKRARKYPISAILISDSDPTPQKQPPESSFTPIFVPETPLSDD 63
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,465,687
Number of Sequences: 28952
Number of extensions: 181005
Number of successful extensions: 651
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 621
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 650
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1584903024
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -