BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0071 (416 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g24140.1 68414.m03045 matrixin family protein similar to matr... 77 6e-15 At1g59970.1 68414.m06755 matrixin family protein similar to SP|P... 76 8e-15 At2g45040.1 68415.m05607 matrix metalloproteinase nearly identic... 76 1e-14 At4g16640.1 68417.m02515 matrix metalloproteinase, putative meta... 69 1e-12 At1g70170.1 68414.m08074 matrixin family protein similar to SP|P... 65 2e-11 At3g01180.1 68416.m00023 glycogen synthase, putative similar to ... 31 0.41 At1g77600.1 68414.m09035 expressed protein weak similarity to Pd... 29 1.3 At5g35970.1 68418.m04332 DNA-binding protein, putative similar t... 28 2.9 At5g52380.1 68418.m06499 zinc knuckle (CCHC-type) family protein... 27 3.8 At5g12360.1 68418.m01454 expressed protein ; expression supporte... 27 5.1 At5g45480.1 68418.m05587 expressed protein contains Pfam domain,... 27 6.7 At1g58370.1 68414.m06640 glycosyl hydrolase family 10 protein / ... 27 6.7 >At1g24140.1 68414.m03045 matrixin family protein similar to matrix metalloproteinase [Cucumis sativus] GI:7159629; contains InterPro accession IPR001818: Matrixin Length = 384 Score = 76.6 bits (180), Expect = 6e-15 Identities = 49/128 (38%), Positives = 60/128 (46%), Gaps = 10/128 (7%) Frame = +2 Query: 2 LVPLTFTQ-KRSGQVHIEIRFEKGEHXXXXXXXXXXXTLAHAYFPVYGGDAHFDDAEMWS 178 + PLTFT+ +R I I F GEH TLAHA+ P G H D E W Sbjct: 203 VTPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTG-HFHLDGEENWI 261 Query: 179 INSRRG---------TNLFQVAAHEFGHSLGLSHSDVRSALMAPFYRGYDPAFQLDQDDV 331 ++ G +L VA HE GH LGL HS V ++M P R L DDV Sbjct: 262 VSGEGGDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDV 321 Query: 332 QGIQSLYG 355 +G+Q LYG Sbjct: 322 EGVQYLYG 329 >At1g59970.1 68414.m06755 matrixin family protein similar to SP|P29136 Metalloendoproteinase 1 precursor (EC 3.4.24.-) (SMEP1) {Glycine max}; contains InterPro accession IPR001818: Matrixin Length = 360 Score = 76.2 bits (179), Expect = 8e-15 Identities = 54/135 (40%), Positives = 64/135 (47%), Gaps = 11/135 (8%) Frame = +2 Query: 2 LVPLTFTQKRSG-QVHIEIRFEKGEHXXXXXXXXXXXTLAHAYFPVYGGDAHFDDAEMWS 178 + PL FT+ S + I I F GEH TLAHA P G H D E W Sbjct: 187 VTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGM-LHLDGDEDWL 245 Query: 179 IN----SRR------GTNLFQVAAHEFGHSLGLSHSDVRSALMAPFYRGYDPAFQLDQDD 328 I+ SRR +L VA HE GH LGL HS V A+M P G D +L +DD Sbjct: 246 ISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDD 305 Query: 329 VQGIQSLYGHKTQTD 373 ++GIQ LYG D Sbjct: 306 IEGIQHLYGGNPNGD 320 >At2g45040.1 68415.m05607 matrix metalloproteinase nearly identical to metalloproteinase [Arabidopsis thaliana] GI:3128477; contains InterPro accession IPR001818: Matrixin Length = 342 Score = 75.8 bits (178), Expect = 1e-14 Identities = 44/124 (35%), Positives = 61/124 (49%), Gaps = 6/124 (4%) Frame = +2 Query: 2 LVPLTFTQKRSGQV-HIEIRFEKGEHXXXXXXXXXXXTLAHAYFPVYGGDAHFDDAEMWS 178 ++P++F + + I+I F G+H LAH + P G H D AE W+ Sbjct: 174 VIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPE-NGRLHLDKAETWA 232 Query: 179 IN-----SRRGTNLFQVAAHEFGHSLGLSHSDVRSALMAPFYRGYDPAFQLDQDDVQGIQ 343 ++ S +L VA HE GH LGL HS V+ A M P + L+ DDV G+Q Sbjct: 233 VDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQ 292 Query: 344 SLYG 355 SLYG Sbjct: 293 SLYG 296 >At4g16640.1 68417.m02515 matrix metalloproteinase, putative metalloproteinase [Arabidopsis thaliana] GI:3128477; contains InterPro accession IPR001818: Matrixin Length = 364 Score = 69.3 bits (162), Expect = 1e-12 Identities = 44/125 (35%), Positives = 58/125 (46%), Gaps = 7/125 (5%) Frame = +2 Query: 2 LVPLTFTQKRS-GQVHIEIRFEKGEHXXXXXXXXXXXTLAHAYFPVYGGDAHFDDAEMWS 178 ++P++F + ++I F G+H TLAHA+ P G H D AE W Sbjct: 196 VIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPE-NGRLHLDAAETWI 254 Query: 179 IN------SRRGTNLFQVAAHEFGHSLGLSHSDVRSALMAPFYRGYDPAFQLDQDDVQGI 340 ++ S +L VA HE GH LGL HS SA+M P R L DDV G+ Sbjct: 255 VDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGV 314 Query: 341 QSLYG 355 LYG Sbjct: 315 LKLYG 319 >At1g70170.1 68414.m08074 matrixin family protein similar to SP|P29136 Metalloendoproteinase 1 precursor (EC 3.4.24.-) (SMEP1) {Glycine max}; contains InterPro accession IPR001818: Matrixin Length = 378 Score = 65.3 bits (152), Expect = 2e-11 Identities = 45/124 (36%), Positives = 53/124 (42%), Gaps = 9/124 (7%) Frame = +2 Query: 11 LTFTQKRSGQVH-IEIRFEKGEHXXXXXXXXXXXTLAHAYFPVYGGDAHFDDAEMWSINS 187 L FT S I I F G+H TLAHA+ P G H D E W ++ Sbjct: 202 LNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPP-SGKFHLDADENWVVSG 260 Query: 188 --------RRGTNLFQVAAHEFGHSLGLSHSDVRSALMAPFYRGYDPAFQLDQDDVQGIQ 343 +L VA HE GH LGL HS V ++M P L DDV+GIQ Sbjct: 261 DLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQ 320 Query: 344 SLYG 355 LYG Sbjct: 321 YLYG 324 >At3g01180.1 68416.m00023 glycogen synthase, putative similar to glycogen synthase Q43847 from [Solanum tuberosum] Length = 792 Score = 30.7 bits (66), Expect = 0.41 Identities = 11/43 (25%), Positives = 21/43 (48%) Frame = -2 Query: 214 YLEKISSSTGIDGPHLSVIKMGITSIHREISVGEGASWTIKRI 86 YL+ + G H ++ G+ + R ++V G SW +K + Sbjct: 491 YLDSFKLYDPVGGEHFNIFAAGLKAADRVLTVSHGYSWEVKTL 533 >At1g77600.1 68414.m09035 expressed protein weak similarity to Pds5 (GI:16751524) [Schizosaccharomyces pombe]; weak similarity to androgen-induced prostate proliferative shutoff associated protein (GI:4559410) [Homo sapiens] Length = 1285 Score = 29.1 bits (62), Expect = 1.3 Identities = 12/25 (48%), Positives = 17/25 (68%) Frame = +1 Query: 235 LAGSLTQRCKIRIDGAVLSWLRSCF 309 LA SL +RC R++ + S+L SCF Sbjct: 226 LASSLIERCADRLEPLICSFLTSCF 250 >At5g35970.1 68418.m04332 DNA-binding protein, putative similar to SWISS-PROT:Q60560 DNA-binding protein SMUBP-2 (Immunoglobulin MU binding protein 2, SMUBP-2) [Mesocricetus auratus] Length = 961 Score = 27.9 bits (59), Expect = 2.9 Identities = 11/23 (47%), Positives = 16/23 (69%) Frame = -2 Query: 217 CYLEKISSSTGIDGPHLSVIKMG 149 C L +S+STG+ G HL + K+G Sbjct: 324 CNLYAVSTSTGLGGMHLVLFKVG 346 >At5g52380.1 68418.m06499 zinc knuckle (CCHC-type) family protein contains Pfam domain, PF00098: Zinc knuckle Length = 268 Score = 27.5 bits (58), Expect = 3.8 Identities = 13/28 (46%), Positives = 15/28 (53%) Frame = -1 Query: 149 HHLHTPGNKRGRGCLLDHQKDLRHRARL 66 H L PG K G GC + H K H A+L Sbjct: 63 HPLRVPGMKPGEGCFICHSK--THIAKL 88 >At5g12360.1 68418.m01454 expressed protein ; expression supported by MPSS Length = 194 Score = 27.1 bits (57), Expect = 5.1 Identities = 10/27 (37%), Positives = 16/27 (59%), Gaps = 2/27 (7%) Frame = -3 Query: 213 TWKRL--VPLRELMDHISASSKWASPP 139 +W L +P+ E D + S++W SPP Sbjct: 135 SWDELKEIPMEEFSDLLECSTQWRSPP 161 >At5g45480.1 68418.m05587 expressed protein contains Pfam domain, PF04578: Protein of unknown function, DUF594 Length = 877 Score = 26.6 bits (56), Expect = 6.7 Identities = 24/66 (36%), Positives = 33/66 (50%), Gaps = 7/66 (10%) Frame = +2 Query: 158 DDAEMWSINSRRGTNLFQVAAHEFG---HSLGLSH-SDVRS---ALMAPFYRGYDPAFQL 316 DD+EMW I S+ L AA + G H+ LS ++ S LMA F G FQ+ Sbjct: 807 DDSEMWKIMSQVWVELLSYAATKCGAIEHAAQLSKGGELISFVWLLMAHF--GLGDQFQI 864 Query: 317 DQDDVQ 334 +Q D + Sbjct: 865 NQGDAR 870 >At1g58370.1 68414.m06640 glycosyl hydrolase family 10 protein / carbohydrate-binding domain-containing protein similar to (1,4)-beta-xylan endohydrolase GI:5306060 from [Triticum aestivum] ; contains Pfam profiles PF00331: Glycosyl hydrolase family 10, PF02018: Carbohydrate binding domain Length = 917 Score = 26.6 bits (56), Expect = 6.7 Identities = 19/75 (25%), Positives = 37/75 (49%), Gaps = 9/75 (12%) Frame = -2 Query: 340 NSLDVVLIELESRIVTTIKRRHQCGSYIAV---*ET-----QRVTKLMR-CYLEKISSST 188 N + ++ ES + + +CGSY+ V ET Q +T ++ C L K+S++ Sbjct: 46 NCCEAFVVTAESNVSHGVLDPSKCGSYVVVKNRKETWQGLEQDITNRVKPCSLYKVSATV 105 Query: 187 GIDGPHLSVIKMGIT 143 + GP ++++ T Sbjct: 106 AVSGPVHGLVEVMAT 120 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,343,007 Number of Sequences: 28952 Number of extensions: 187030 Number of successful extensions: 511 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 492 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 504 length of database: 12,070,560 effective HSP length: 74 effective length of database: 9,928,112 effective search space used: 635399168 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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