BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0064 (490 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAP8A3.09c |paa1||protein phosphatase regulatory subunit Paa1|S... 138 4e-34 SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 42 7e-05 SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces pomb... 29 0.50 SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 28 0.87 SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 27 2.0 SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 26 3.5 SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac... 25 6.1 SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||M... 25 6.1 SPBC3B8.09 |||U3 snoRNP-associated protein Utp3 |Schizosaccharom... 25 6.1 SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein Vps33|S... 25 6.1 SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces ... 25 8.1 SPAC30.03c |tsn1|tsn, mug90|translin|Schizosaccharomyces pombe|c... 25 8.1 SPCC74.02c |||mRNA cleavage and polyadenylation specificity fact... 25 8.1 >SPAP8A3.09c |paa1||protein phosphatase regulatory subunit Paa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 590 Score = 138 bits (334), Expect = 4e-34 Identities = 71/156 (45%), Positives = 103/156 (66%) Frame = +2 Query: 23 LLAPEDMEQHVMPTVRARAGDTSWRVRYMVADKFVELQQAVGPELARTDLAQIFQALLKD 202 L + ++ +++ +R+ D+SWR RYMVA FV+L + VGP L + +L + F L+KD Sbjct: 236 LKSDSEIRHYLLQPLRSFVSDSSWRTRYMVAANFVKLAKVVGPSLIKDELIKPFVLLMKD 295 Query: 203 SEAEVRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVIMGLS 382 +E EVR A A ++ FC LDK I++ I+P I++L+ D QHV++AL I L+ Sbjct: 296 TEQEVRRAIATQIPGFCELLDK----RIVLEEIIPVIQELINDPAQHVRAALGMNIGALA 351 Query: 383 PIVGRQNTIEHLLPLFLTQLKDECPEVRLNIISNLE 490 P +G++ T E+LLP+FL LKDE PEVRLNIIS LE Sbjct: 352 PQLGKEKTTEYLLPMFLELLKDENPEVRLNIISKLE 387 Score = 46.8 bits (106), Expect = 2e-06 Identities = 34/160 (21%), Positives = 63/160 (39%) Frame = +2 Query: 11 VVASLLAPEDMEQHVMPTVRARAGDTSWRVRYMVADKFVELQQAVGPELARTDLAQIFQA 190 VV ++ E + Q ++P + A D WRVR + D L Q +G E + + + Sbjct: 388 VVNKVVGIELLSQSLLPAIVTLAEDKQWRVRLAIIDYIPLLAQQLGVEFFNEKMGNLCMS 447 Query: 191 LLKDSEAEVRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVI 370 L+D +R AA ++ L + I+P+ + N + I Sbjct: 448 WLEDHVYSIREAAIKNLR----KLTEIFGLEWATETIIPKFLAMRSHPNYLYRMTTIFAI 503 Query: 371 MGLSPIVGRQNTIEHLLPLFLTQLKDECPEVRLNIISNLE 490 ++P + + + +LP + D P +R N+ E Sbjct: 504 SEIAPALNAEVIEKQILPTLEQLVNDPIPNIRFNVAKAFE 543 Score = 45.6 bits (103), Expect = 4e-06 Identities = 35/155 (22%), Positives = 66/155 (42%) Frame = +2 Query: 14 VASLLAPEDMEQHVMPTVRARAGDTSWRVRYMVADKFVELQQAVGPELARTDLAQIFQAL 193 +A ++ P ++ ++ DT VR +A + + + + ++ + Q L Sbjct: 272 LAKVVGPSLIKDELIKPFVLLMKDTEQEVRRAIATQIPGFCELLDKRIVLEEIIPVIQEL 331 Query: 194 LKDSEAEVRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVIM 373 + D VRAA + L K ++ M L +KD N V+ + S + Sbjct: 332 INDPAQHVRAALGMNIGALAPQLGKEKTTEYLLPMFLELLKD----ENPEVRLNIISKLE 387 Query: 374 GLSPIVGRQNTIEHLLPLFLTQLKDECPEVRLNII 478 ++ +VG + + LLP +T +D+ VRL II Sbjct: 388 VVNKVVGIELLSQSLLPAIVTLAEDKQWRVRLAII 422 Score = 27.9 bits (59), Expect = 0.87 Identities = 29/122 (23%), Positives = 50/122 (40%), Gaps = 1/122 (0%) Frame = +2 Query: 107 MVADKFVELQQAVGPELARTDLAQIFQALLKD-SEAEVRAAAAGKVKDFCMNLDKAHQEH 283 +V DK V+ V L++ L Q F L++ S AE + A +C ++ + Sbjct: 103 VVRDKAVDSLNKVCICLSQEQLEQYFVPLVQRLSTAEWFTSRASSAGLYCAAYSQS-ENP 161 Query: 284 IIMTMILPQIKDLVCDANQHVKSALASVIMGLSPIVGRQNTIEHLLPLFLTQLKDECPEV 463 + + L D V+ A+ +V +Q I+ +PLF + D+ V Sbjct: 162 AVKVSLRQSFSHLCHDEAPMVRRPAATNCAKFVFLVTKQEAIDEFIPLFNSLSNDDQDSV 221 Query: 464 RL 469 RL Sbjct: 222 RL 223 >SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizosaccharomyces pombe|chr 2|||Manual Length = 1706 Score = 41.5 bits (93), Expect = 7e-05 Identities = 26/98 (26%), Positives = 48/98 (48%) Frame = +2 Query: 191 LLKDSEAEVRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVI 370 LL DS + VR + + C+ KA +I++ ++ + D + ++ A I Sbjct: 600 LLADSSSIVRRSLLNALAPLCVFFGKAKSNDLILSHLITYLND----TDWMLRCAFFESI 655 Query: 371 MGLSPIVGRQNTIEHLLPLFLTQLKDECPEVRLNIISN 484 GLS +G ++ E++LPL L L D P V +++ + Sbjct: 656 TGLSIFIGPRSVDEYILPLMLQALVDPEPAVLESVLGS 693 Score = 29.1 bits (62), Expect = 0.38 Identities = 23/96 (23%), Positives = 38/96 (39%) Frame = +2 Query: 83 DTSWRVRYMVADKFVELQQAVGPELARTDLAQIFQALLKDSEAEVRAAAAGKVKDFCMNL 262 DT W +R + L +GP + + L D E V + G F + Sbjct: 642 DTDWMLRCAFFESITGLSIFIGPRSVDEYILPLMLQALVDPEPAVLESVLG---SFSGLI 698 Query: 263 DKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVI 370 + E +++ IL + LV N +++ A SVI Sbjct: 699 ELHLFEKLVVVDILQLVLPLVAVPNAYIRRAALSVI 734 Score = 25.8 bits (54), Expect = 3.5 Identities = 14/62 (22%), Positives = 34/62 (54%), Gaps = 1/62 (1%) Frame = +2 Query: 296 MILPQIKDLVCDAN-QHVKSALASVIMGLSPIVGRQNTIEHLLPLFLTQLKDECPEVRLN 472 ++LP + + N + K S++ LS + ++ ++ +LP +T L+D+ +VR++ Sbjct: 431 VLLPIVLSTIRHVNTRESKINALSLVQILSRNICDESKLDTVLPFVMTLLRDQYADVRIS 490 Query: 473 II 478 + Sbjct: 491 AL 492 >SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces pombe|chr 1|||Manual Length = 910 Score = 28.7 bits (61), Expect = 0.50 Identities = 23/68 (33%), Positives = 28/68 (41%), Gaps = 2/68 (2%) Frame = +2 Query: 59 PTVRARAGDTSWRVRYMVADKFVELQQAVGPELARTD--LAQIFQALLKDSEAEVRAAAA 232 PTV D +V+ L V P LA+ D L QI KD EVR +A Sbjct: 651 PTVDVPDRDFLVTTLDLVSGIIQALGSQVSPLLAQADPPLGQIIGICAKDEVPEVRQSAY 710 Query: 233 GKVKDFCM 256 + D CM Sbjct: 711 ALLGDMCM 718 >SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pombe|chr 2|||Manual Length = 1067 Score = 27.9 bits (59), Expect = 0.87 Identities = 27/96 (28%), Positives = 50/96 (52%), Gaps = 4/96 (4%) Frame = +2 Query: 167 DLAQIFQALLKDSEAEVRAAAAGKVKDFC-MNLDKAHQEHIIMTMILPQIKDLVCDANQH 343 D+ +F A L+DSE EVR+ AA + C + + E++ IL +++ + Sbjct: 894 DVFSLFMAALEDSEGEVRSNAAYSMGLLCQFSTEDLSSEYL---NILQKLQPFF--TQEV 948 Query: 344 VKSALASVIMGLSP-IVGRQNTI--EHLLPLFLTQL 442 ++AL + I +S I+ QN I + +LP+ ++L Sbjct: 949 FRTALDNAIGCISRLILHNQNAIPVDQVLPIVFSKL 984 >SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 1106 Score = 26.6 bits (56), Expect = 2.0 Identities = 9/42 (21%), Positives = 20/42 (47%) Frame = -2 Query: 420 RRCSIVFCLPTMGLRPMMTEARADLTCWLASHTKSLICGKIM 295 R S+++CLP+ +P+ + + +H + C +M Sbjct: 387 REASLLYCLPSTDFQPLFVSEKLSIQEVTYAHCVRIFCEHVM 428 >SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe|chr 1|||Manual Length = 570 Score = 25.8 bits (54), Expect = 3.5 Identities = 10/30 (33%), Positives = 18/30 (60%) Frame = -1 Query: 322 QVLDLWQDHGHDDVFLMSFIQVHTEVLDLP 233 ++LDL++D ++ ++VH VLD P Sbjct: 525 ELLDLFKDEEFGKADVIQLVEVHMPVLDAP 554 >SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosaccharomyces pombe|chr 3|||Manual Length = 1140 Score = 25.0 bits (52), Expect = 6.1 Identities = 14/47 (29%), Positives = 24/47 (51%) Frame = +2 Query: 242 KDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVIMGLS 382 ++F L HQE + + P ++L N+H KS ++ + GLS Sbjct: 1001 RNFTGKLVIKHQEEFLEPRVYPANRNLATAHNRHEKSKVS--VQGLS 1045 >SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||Manual Length = 685 Score = 25.0 bits (52), Expect = 6.1 Identities = 12/33 (36%), Positives = 16/33 (48%) Frame = +2 Query: 287 IMTMILPQIKDLVCDANQHVKSALASVIMGLSP 385 I T+ + I+ L D H KS MGL+P Sbjct: 9 IDTLAINTIRTLAVDTTAHAKSGHPGAPMGLAP 41 >SPBC3B8.09 |||U3 snoRNP-associated protein Utp3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 597 Score = 25.0 bits (52), Expect = 6.1 Identities = 10/21 (47%), Positives = 15/21 (71%) Frame = +2 Query: 128 ELQQAVGPELARTDLAQIFQA 190 EL Q + P+L RT+L +I +A Sbjct: 191 ELVQQISPDLPRTELLKILEA 211 >SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein Vps33|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 25.0 bits (52), Expect = 6.1 Identities = 16/61 (26%), Positives = 27/61 (44%) Frame = +2 Query: 170 LAQIFQALLKDSEAEVRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVK 349 L++I + L D E +A +++DF L EH + + + LV QH K Sbjct: 294 LSKIARKLSSDFEGRRQAKTVNQIRDFVSKLGSLQSEHTSLNIHTGLAETLV----QHTK 349 Query: 350 S 352 + Sbjct: 350 N 350 >SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 582 Score = 24.6 bits (51), Expect = 8.1 Identities = 10/35 (28%), Positives = 18/35 (51%) Frame = +1 Query: 337 PTRQVCPSLRHHGPEPHSGQTEHY*TSPAAVPHTT 441 P+ CPS++ GP H+ Q + + +P T+ Sbjct: 314 PSNCSCPSVKSSGPSYHAEQEVNVNSYNGGIPSTS 348 >SPAC30.03c |tsn1|tsn, mug90|translin|Schizosaccharomyces pombe|chr 1|||Manual Length = 236 Score = 24.6 bits (51), Expect = 8.1 Identities = 9/21 (42%), Positives = 14/21 (66%) Frame = -1 Query: 448 ILQLCEEQRQEMFNSVLSAHY 386 +L LC E ++ NSV+S +Y Sbjct: 162 VLSLCSELARQSVNSVISGNY 182 >SPCC74.02c |||mRNA cleavage and polyadenylation specificity factor complex associated protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 710 Score = 24.6 bits (51), Expect = 8.1 Identities = 14/49 (28%), Positives = 25/49 (51%), Gaps = 1/49 (2%) Frame = +2 Query: 344 VKSALASVIMGLSPIVGRQNTI-EHLLPLFLTQLKDECPEVRLNIISNL 487 +KS I P+ + T+ E L P+ + Q EC + +N++SN+ Sbjct: 177 LKSKSRQKISSSDPLEDLRLTLTECLNPINIVQAPKECAAILVNLMSNI 225 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,932,062 Number of Sequences: 5004 Number of extensions: 34805 Number of successful extensions: 137 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 127 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 133 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 190087364 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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