BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0061 (673 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|... 99 5e-22 SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma... 38 0.001 SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharo... 29 0.46 SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 27 1.9 SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun... 27 2.5 SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr... 26 4.3 SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pom... 26 5.7 SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 26 5.7 SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor c... 25 7.5 SPAC16C9.03 |||export adaptor|Schizosaccharomyces pombe|chr 1|||... 25 7.5 SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual 25 7.5 SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 7.5 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 25 9.9 SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc... 25 9.9 >SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|||Manual Length = 137 Score = 99.1 bits (236), Expect = 5e-22 Identities = 55/140 (39%), Positives = 81/140 (57%) Frame = +1 Query: 64 SGVTVSDVCKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTG 243 SGV VS C ++E+K K RYVVF + D K V + +++ FL DL + Sbjct: 4 SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDLPEK--- 60 Query: 244 ECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQ 423 +CRY ++DFE+ + +G + K+ +SW PD A +K KM+YSSS D L+++ G+ Sbjct: 61 DCRYAIYDFEF-NLGEGV-----RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIG 114 Query: 424 KYIQATDLSEASQEAVEEKL 483 IQATD SE + E V EK+ Sbjct: 115 TDIQATDFSEVAYETVLEKV 134 >SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Manual Length = 328 Score = 37.9 bits (84), Expect = 0.001 Identities = 28/86 (32%), Positives = 44/86 (51%) Frame = +1 Query: 130 RYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDFEYTHQCQGTSEAS 309 R + I +E DV+T+ E++ E D +K EC G + + + S Sbjct: 26 RAAIISISNENSFDVKTMVEKSESIES---DFKK--VRECLLGSEEPAFVL----VYDDS 76 Query: 310 KKQKLFLMSWCPDTAKVKKKMLYSSS 387 KK L L+S+ P+ A V++KMLY+SS Sbjct: 77 KKNLLQLISYVPENANVRRKMLYASS 102 Score = 28.7 bits (61), Expect = 0.81 Identities = 16/56 (28%), Positives = 32/56 (57%), Gaps = 5/56 (8%) Frame = +1 Query: 319 KLFLMSWCPDTAKVKKKMLYSSS----FDALKKSL-VGVQKYIQATDLSEASQEAV 471 K+ + CP A VK +M+YSSS D++K L + + I++ D ++ +++ + Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEI 299 >SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 29.5 bits (63), Expect = 0.46 Identities = 17/63 (26%), Positives = 30/63 (47%) Frame = +1 Query: 295 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVE 474 T++ LF++ W P M+Y+S+ + + V K +A D + + EAV+ Sbjct: 79 TTDGRLSTPLFMIYWRPSATPNDLSMIYASA-KVWFQDVSQVHKVFEARDSEDITSEAVD 137 Query: 475 EKL 483 E L Sbjct: 138 EFL 140 >SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 314 Score = 27.5 bits (58), Expect = 1.9 Identities = 24/95 (25%), Positives = 36/95 (37%) Frame = +1 Query: 265 DFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATD 444 +F+ Q T A+K + P T K + ++ A KK + G +KY + Sbjct: 120 NFKRRTQFAFTGRATKSNAGVMDVQSPSTMSTSKNNVRNAERPASKKPVFGSKKYFDVIN 179 Query: 445 LSEASQEAVEEKLRATDRQ*TAFTHELATKPNPLS 549 S + E K R DR + FT LS Sbjct: 180 DSNVENKE-ETKDRNLDRALSKFTQSREKNEQNLS 213 >SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 214 Score = 27.1 bits (57), Expect = 2.5 Identities = 10/26 (38%), Positives = 19/26 (73%) Frame = +1 Query: 385 SFDALKKSLVGVQKYIQATDLSEASQ 462 S +AL++ L G + Y+Q+TD++ S+ Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMSK 213 >SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr 2|||Manual Length = 319 Score = 26.2 bits (55), Expect = 4.3 Identities = 26/136 (19%), Positives = 60/136 (44%), Gaps = 1/136 (0%) Frame = +1 Query: 91 KTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDF 270 K + + ++ + K ++ +D K+ D + + + EY F ++L+ TG+ Y DF Sbjct: 108 KCSSDFLEANVKSQFTTCENKDSKE-DYQILHNKRLEYVSFNDELKSLETGQPLYCFQDF 166 Query: 271 EYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLS 450 + + G S+K ++ A V K ++ +K++ K ++ + Sbjct: 167 Q--KKVHGPPALSEKPGKCILK-DKTNAHVNKIPQDEVNYSLPQKNITIFSKELKENEFE 223 Query: 451 EASQ-EAVEEKLRATD 495 ++ E EEK + ++ Sbjct: 224 SINEGETEEEKAKTSN 239 >SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 447 Score = 25.8 bits (54), Expect = 5.7 Identities = 17/60 (28%), Positives = 28/60 (46%), Gaps = 3/60 (5%) Frame = +3 Query: 447 LGSVSGGRRREAPRHRSPINSIYTRARDE---TEPALRHSCPDDTRPRHH*PLSIITKEN 617 LG G ++ E+ R + P S + EP +RHS RP+ H S +T+++ Sbjct: 20 LGEEDGRQKGESSRKKRPPLSRKNPSNVSFWSNEPIIRHSSVKTDRPQFHRADSTVTEQS 79 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 25.8 bits (54), Expect = 5.7 Identities = 12/44 (27%), Positives = 23/44 (52%), Gaps = 5/44 (11%) Frame = -3 Query: 338 HDIRKSFCFLLASDVPWHWCVYSKSNRPY-----LHSPVPPFCR 222 HD+ +++ S +H C ++S P+ LH+P+P C+ Sbjct: 107 HDVASHPSYMVQSPTSYHACSNNQSPFPHSHHPPLHNPLPVSCQ 150 >SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor complex subunit Rrn5|Schizosaccharomyces pombe|chr 1|||Manual Length = 556 Score = 25.4 bits (53), Expect = 7.5 Identities = 22/92 (23%), Positives = 39/92 (42%), Gaps = 4/92 (4%) Frame = -3 Query: 554 VSESGFGFVASSCVNAVYWRSVARSFSSTAS*DASERSVAWMYF*TPTR---DFFRASNE 384 + E +G ++ SCV+ YW + + A +R + + + P++ R N Sbjct: 56 LEEEQWGVLSGSCVDGTYWSAEEKELFFQAVARNGKRDLDLIAYSIPSKSAVQIERYINA 115 Query: 383 LEYNIFFLTLAVSGHHDIR-KSFCFLLASDVP 291 LE + +L H D +S C L D+P Sbjct: 116 LENELRWL----RNHVDASVRSQCLLKYEDIP 143 >SPAC16C9.03 |||export adaptor|Schizosaccharomyces pombe|chr 1|||Manual Length = 498 Score = 25.4 bits (53), Expect = 7.5 Identities = 11/50 (22%), Positives = 27/50 (54%), Gaps = 1/50 (2%) Frame = +1 Query: 85 VCKTTYEEIKKDKKH-RYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQK 231 + K TY + KK ++ R ++ + + + + + +YE FL++L++ Sbjct: 397 IVKKTYPQTKKKNRNWRLKTIGMQKAEDVKKQDIERQERDYELFLQNLEE 446 >SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 366 Score = 25.4 bits (53), Expect = 7.5 Identities = 10/22 (45%), Positives = 11/22 (50%) Frame = +1 Query: 73 TVSDVCKTTYEEIKKDKKHRYV 138 TV D C + IK KH YV Sbjct: 316 TVEDFCNNIHSSIKSQFKHAYV 337 >SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 357 Score = 25.4 bits (53), Expect = 7.5 Identities = 13/46 (28%), Positives = 20/46 (43%), Gaps = 2/46 (4%) Frame = -3 Query: 290 WHWCVYSKSNRPY--LHSPVPPFCRSSRNCSYSALRSPTVSTSICF 159 W++ Y+ S R Y VPP+CR +R+ + CF Sbjct: 122 WNFVDYALSLRKYGWTDDVVPPYCRKVLTYKSLGIRTAVYVGAFCF 167 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 25.0 bits (52), Expect = 9.9 Identities = 14/39 (35%), Positives = 17/39 (43%) Frame = +3 Query: 480 APRHRSPINSIYTRARDETEPALRHSCPDDTRPRHH*PL 596 APR R P ++ T A E P + P TR PL Sbjct: 24 APRKREPARTVSTPAFMEPAPVSKKPLPPPTRRLPRKPL 62 >SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc7|Schizosaccharomyces pombe|chr 2|||Manual Length = 1062 Score = 25.0 bits (52), Expect = 9.9 Identities = 14/41 (34%), Positives = 20/41 (48%) Frame = +3 Query: 495 SPINSIYTRARDETEPALRHSCPDDTRPRHH*PLSIITKEN 617 SP+++ D ++PAL S P+H LS KEN Sbjct: 391 SPLSTPLKAQFDPSKPALNRSIDHQKTPQHKRYLSTEFKEN 431 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,366,251 Number of Sequences: 5004 Number of extensions: 45158 Number of successful extensions: 157 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 152 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 154 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 307866294 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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