BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0058 (423 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC664.05 |rpl13||60S ribosomal protein L13|Schizosaccharomyces... 153 1e-38 SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces pomb... 28 0.52 SPAC2F3.12c |||conserved eukaryotic protein|Schizosaccharomyces ... 25 3.7 SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccha... 25 4.8 SPBC14C8.06 |arc1|sop2|ARP2/3 actin-organizing complex subunit S... 25 6.4 SPCP1E11.11 |||Puf family RNA-binding protein|Schizosaccharomyce... 24 8.5 SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom... 24 8.5 >SPAC664.05 |rpl13||60S ribosomal protein L13|Schizosaccharomyces pombe|chr 1|||Manual Length = 208 Score = 153 bits (370), Expect = 1e-38 Identities = 76/133 (57%), Positives = 93/133 (69%), Gaps = 1/133 (0%) Frame = +1 Query: 28 IPNGHFHKDWQRFVKTWFNQPARRYRRKQNRIXXXXXXXXXXXXXXLRPIVRCPTVRYHT 207 +PN HFHKDWQR+VKTWFNQP R+ RR+Q R +RP V+ PT+RY+ Sbjct: 9 LPNAHFHKDWQRYVKTWFNQPGRKLRRRQAR-QTKAAKIAPRPVEAIRPAVKPPTIRYNM 67 Query: 208 KVRAGRGFTLREIRAAGLNPVFARTIGIAVDPRRRNKSVESLQINVQRIKEYRARLILFP 387 KVRAGRGFTL E++AAG++ A TIGI VD RRRN+S ESLQ NV+RIK Y A LI+FP Sbjct: 68 KVRAGRGFTLEELKAAGVSRRVASTIGIPVDHRRRNRSEESLQRNVERIKVYLAHLIVFP 127 Query: 388 -KGKKVLKGDANE 423 K + KGDA + Sbjct: 128 RKAGQPKKGDATD 140 >SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces pombe|chr 2|||Manual Length = 1283 Score = 28.3 bits (60), Expect = 0.52 Identities = 16/44 (36%), Positives = 20/44 (45%), Gaps = 4/44 (9%) Frame = -1 Query: 240 TKSESSTGAYFSM----VPNSWASHYRT*RPSCRTWSYGLSFLY 121 T +STG+Y M + W S T C TWSY S+ Y Sbjct: 1215 TVQGTSTGSYICMPHFQIQYDWCSAGVTDMSECNTWSYQKSYDY 1258 >SPAC2F3.12c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 279 Score = 25.4 bits (53), Expect = 3.7 Identities = 12/25 (48%), Positives = 17/25 (68%) Frame = +2 Query: 221 VEDSLFVKLGPQD*TQYLPERLELL 295 +ED+LF +L D T Y +RLE+L Sbjct: 95 LEDALFSQLDEFDDTAYREQRLEML 119 >SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccharomyces pombe|chr 3|||Manual Length = 721 Score = 25.0 bits (52), Expect = 4.8 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +2 Query: 32 LMDISTRIGKDLLKLGLTSQLDDTAES 112 L+DIS R G+D L S+L D +S Sbjct: 556 LLDISKRKGRDSFVAALNSRLKDIKDS 582 >SPBC14C8.06 |arc1|sop2|ARP2/3 actin-organizing complex subunit Sop2|Schizosaccharomyces pombe|chr 2|||Manual Length = 377 Score = 24.6 bits (51), Expect = 6.4 Identities = 9/19 (47%), Positives = 15/19 (78%) Frame = -2 Query: 257 PAALISRRVNPLPARTLVW 201 P ALI+ +++ LP R+L+W Sbjct: 241 PRALITVKLSQLPLRSLLW 259 >SPCP1E11.11 |||Puf family RNA-binding protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 642 Score = 24.2 bits (50), Expect = 8.5 Identities = 11/33 (33%), Positives = 19/33 (57%), Gaps = 1/33 (3%) Frame = -3 Query: 355 LFFEH*FAMIQQTCCVYGDLQQFQSF-GQILGS 260 L F+H + + QTC +G QQ ++ ++ GS Sbjct: 169 LVFKHDMSRVVQTCVKFGSKQQRETICAELAGS 201 >SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 24.2 bits (50), Expect = 8.5 Identities = 12/45 (26%), Positives = 20/45 (44%) Frame = -1 Query: 309 STGIYSNSNRSGKYWVQSCGPNFTKSESSTGAYFSMVPNSWASHY 175 ST YSN R + + C K+E + + PN +++ Y Sbjct: 368 STVSYSNPVRQSLFTFEDCKRKLPKAECAAQRLKEIYPNMFSTGY 412 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,826,127 Number of Sequences: 5004 Number of extensions: 36164 Number of successful extensions: 103 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 102 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 102 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 150383836 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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