SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ceN-0034
         (693 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   329   3e-91
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   183   2e-47
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   183   2e-47
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   169   2e-43
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    38   0.001
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi...    34   0.017
SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc...    30   0.36 
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    30   0.36 
SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl...    29   0.84 
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce...    28   1.1  
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual        27   1.9  
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    27   2.6  
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos...    27   3.4  
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar...    27   3.4  
SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pomb...    26   4.5  
SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha...    26   4.5  
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc...    26   5.9  
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos...    25   7.9  
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz...    25   7.9  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  329 bits (808), Expect = 3e-91
 Identities = 147/210 (70%), Positives = 171/210 (81%)
 Frame = +2

Query: 59  MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 238
           MREIVH+QAGQCGNQ+GA FW  I+DEHG+D  G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60

Query: 239 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 418
           PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61  PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120

Query: 419 RKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVV 598
           R+E+E+CD LQGFQ                   KIREEYPDR+M T+SV P+PK SDTVV
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180

Query: 599 EPYNATLSVHQLVENTDETYCIDNEALYDI 688
           EPYNATLS+HQLVEN+DET+CIDNEAL  I
Sbjct: 181 EPYNATLSMHQLVENSDETFCIDNEALSSI 210


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  183 bits (446), Expect = 2e-47
 Identities = 86/217 (39%), Positives = 128/217 (58%), Gaps = 6/217 (2%)
 Frame = +2

Query: 59  MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 220
           MRE++ +  GQ G QIG   WE+   EHGI P G        H ++    +    +++E 
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 221 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 400
             GK+VPR+I VDLEP  +D VR+GP+  +F P+  V G+  A NN+A+GHYT G E++D
Sbjct: 61  GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120

Query: 401 SVLDVVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPK 580
           SVL+ +R+ +++C  LQGF                    ++  EY  +    +SV P+P+
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180

Query: 581 VSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDIC 691
           VS +VVEPYN+ L+ H  ++N+D T+ +DNEA YDIC
Sbjct: 181 VSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDIC 217


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  183 bits (446), Expect = 2e-47
 Identities = 86/213 (40%), Positives = 125/213 (58%), Gaps = 2/213 (0%)
 Frame = +2

Query: 59  MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 232
           MREI+ +  GQ G QIG   WE+   EHGI P G  + ++  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 233 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 412
           YVPR+I VDLEP  +D VR+GP+  +F P+  + G+  A NN+A+GHYT G ELVD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 413 VVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDT 592
            +R+ +++C  LQGF                    ++  EY  +    +SV P+P+VS +
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180

Query: 593 VVEPYNATLSVHQLVENTDETYCIDNEALYDIC 691
           VVEPYN+ L+ H  ++  D T+ +DNE+ YDIC
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDIC 213


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  169 bits (412), Expect = 2e-43
 Identities = 79/212 (37%), Positives = 128/212 (60%), Gaps = 3/212 (1%)
 Frame = +2

Query: 62  REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 241
           REI+ LQAGQCGNQIG++FW+ +  EHGI P G     +   ++R +V++ ++   +Y+P
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62

Query: 242 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 415
           RAIL+DLEP  ++++ S  +G ++ P+N +  ++  GAGNNWA G Y+    + + ++D+
Sbjct: 63  RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121

Query: 416 VRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVP-SPKVSDT 592
           + +E++  D L+GF                    ++ + YP +I+ TYSV P S  VSD 
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181

Query: 593 VVEPYNATLSVHQLVENTDETYCIDNEALYDI 688
           VV+PYN+ L++ +L  N D    +DN AL  I
Sbjct: 182 VVQPYNSLLALKRLTLNADSVVVLDNAALAHI 213


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 38.3 bits (85), Expect = 0.001
 Identities = 35/131 (26%), Positives = 66/131 (50%)
 Frame = +3

Query: 300 SDRSSVRTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLHIPSV 479
           S  S++ ++S S S  P+T+    +T   LSS + SS  S+ + + +I   +S+    S 
Sbjct: 225 SSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSS----SS 280

Query: 480 AAPGPVWAPSSSQRSVKSTPTES*THTQ*SPRPKYQTLSSNHTMRLSQFTS*LKTQTKPT 659
           ++P    +  SS  S  S+PT + + T  S      + SS  T+  S  +S     + PT
Sbjct: 281 SSPTSTSSTISSSSSSSSSPTST-SSTISSSSSSSSSFSS--TLSSSSMSSSSSFSSSPT 337

Query: 660 ASTTRLYTTSA 692
           +S++ + ++S+
Sbjct: 338 SSSSTISSSSS 348


>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 374

 Score = 34.3 bits (75), Expect = 0.017
 Identities = 29/128 (22%), Positives = 63/128 (49%)
 Frame = +3

Query: 309 SSVRTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLHIPSVAAP 488
           + V  T+ S S   +TT    ++    SS T ++  S+ + + + +  +S+    S ++ 
Sbjct: 120 NGVLQTTVSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSS 179

Query: 489 GPVWAPSSSQRSVKSTPTES*THTQ*SPRPKYQTLSSNHTMRLSQFTS*LKTQTKPTAST 668
               + SSS  S  S+ + S + +  S  P   + SS+H+   S  +S   + ++P++S+
Sbjct: 180 SSSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSSTSSSHSSSSSSSSS-SSSSSRPSSSS 238

Query: 669 TRLYTTSA 692
           + + T S+
Sbjct: 239 SFITTMSS 246


>SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase
           Shk1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 658

 Score = 29.9 bits (64), Expect = 0.36
 Identities = 29/105 (27%), Positives = 50/105 (47%)
 Frame = +3

Query: 363 PRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLHIPSVAAPGPVWAPSSSQRSVKSTPT 542
           PR++T++ L S++  S  S+  Q  +    +S L+ PS  AP P  + SSS      T  
Sbjct: 219 PRESTEKPLLSVSALSS-SSHLQPTSATSSSSRLY-PSRPAPTPPASSSSSPLLSSQTVK 276

Query: 543 ES*THTQ*SPRPKYQTLSSNHTMRLSQFTS*LKTQTKPTASTTRL 677
            + ++    P P   + S+++ +R S     L  QT  T+ T  +
Sbjct: 277 TTTSNASRQPSPLVSSKSTDNIIR-SHSPVLLTPQTLSTSETKHI 320


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 29.9 bits (64), Expect = 0.36
 Identities = 35/124 (28%), Positives = 51/124 (41%), Gaps = 1/124 (0%)
 Frame = +3

Query: 321 TTSFSDSPAPAT-TGPRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLHIPSVAAPGPV 497
           +TS +  P P T T   DT    L + + S   S    ++     +S+L  P +    P 
Sbjct: 142 STSSTSIPIPPTSTSSTDTNSNPLPTTSTSCTTST---SIPPTGGSSSLSTP-ITPTVPP 197

Query: 498 WAPSSSQRSVKSTPTES*THTQ*SPRPKYQTLSSNHTMRLSQFTS*LKTQTKPTASTTRL 677
            + SS+   +  T T S T T  SP P   T  +  T   +  +S L T   PT   T  
Sbjct: 198 TSTSSTSIPIPPTSTSS-TDTNSSPLPTTSTSCTTSTSIPTGGSSSLSTPITPTVPPTST 256

Query: 678 YTTS 689
            +TS
Sbjct: 257 SSTS 260


>SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex
           subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 709

 Score = 28.7 bits (61), Expect = 0.84
 Identities = 23/67 (34%), Positives = 33/67 (49%)
 Frame = +3

Query: 309 SSVRTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLHIPSVAAP 488
           SS  T+SFSD  A +   P+ T+       T S++ S  + N ++     N   PS   P
Sbjct: 132 SSPATSSFSDPKAFSAGVPKFTSD------TSSTVSSTPSLNHSL----QNSMPPSTPTP 181

Query: 489 GPVWAPS 509
            PVWAP+
Sbjct: 182 PPVWAPT 188


>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1036

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 28/118 (23%), Positives = 53/118 (44%), Gaps = 1/118 (0%)
 Frame = +3

Query: 321 TTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLHIPSVAAPGPVW 500
           TTS++ + + A++    ++    SSLT SS  ++ + N   +   ++  + S +A     
Sbjct: 60  TTSYNYNTSSASSSSLTSSSAASSSLTSSSSLASSSTNSTTSASPTSSSLTSSSATSSSL 119

Query: 501 APSS-SQRSVKSTPTES*THTQ*SPRPKYQTLSSNHTMRLSQFTS*LKTQTKPTASTT 671
           A SS +  S+ S+   S +    S        SS  +  L+  ++   T   PT+S T
Sbjct: 120 ASSSTTSSSLASSSITSSSLASSSITSSSLASSSTTSSSLASSSTNSTTSATPTSSAT 177


>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 234

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = +2

Query: 362 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 451
           A+GH   G ELV +  D +RK+SE+   L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = +2

Query: 98  NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 199
           N++G    E+++++  +DPT A   + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166


>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
           Pof11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 506

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +2

Query: 293 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 394
           GP+G +F P  F+F  +G    NW+   Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190


>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1131

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 14/41 (34%), Positives = 23/41 (56%)
 Frame = +3

Query: 300 SDRSSVRTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SA 422
           S  SS   T+ S + +   T P  ++  V+SS++ SSM S+
Sbjct: 342 SSSSSASATATSSAESSIATSPITSSSNVVSSISTSSMDSS 382


>SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 258

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 11/32 (34%), Positives = 19/32 (59%)
 Frame = +3

Query: 435 LAIAYRASNLHIPSVAAPGPVWAPSSSQRSVK 530
           LA+ Y    + + +V APG +W+P+  +R  K
Sbjct: 159 LAVRYGPLGIRV-NVCAPGTIWSPAWDERFKK 189


>SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 363

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 11/19 (57%), Positives = 15/19 (78%)
 Frame = +3

Query: 42  KITYTK*GKSFIYRPANVV 98
           +I+YT  G SFIYRP N++
Sbjct: 64  EISYT--GTSFIYRPGNII 80


>SPAC29B12.07 |sec16||multidomain vesicle coat component
           Sec16|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1995

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 26/97 (26%), Positives = 39/97 (40%), Gaps = 1/97 (1%)
 Frame = +3

Query: 318 RTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SAKNQNLAIAYR-ASNLHIPSVAAPGP 494
           R  SF   PA  +  PR       S    + M    N  ++ AY+  + L  P++ A   
Sbjct: 627 RQYSFQPRPATPSNPPRSLPPP--SGQVNAPMSQTPNP-ISFAYQHGTPLATPTMRANSF 683

Query: 495 VWAPSSSQRSVKSTPTES*THTQ*SPRPKYQTLSSNH 605
              P+SS   ++   T +  HT     PK  T +S H
Sbjct: 684 NSYPASSAEPIRRPATTTVGHTPNLYSPKTNTYNSRH 720


>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1063

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 12/44 (27%), Positives = 20/44 (45%)
 Frame = +2

Query: 314  RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 445
            RP   +F     G++   G   +  E  D ++ +  +  ESCDC
Sbjct: 955  RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997


>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 255

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 13/32 (40%), Positives = 16/32 (50%)
 Frame = -1

Query: 351 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 256
           P+   PK  L  R I P GPE  +  + GS S
Sbjct: 20  PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,750,570
Number of Sequences: 5004
Number of extensions: 57162
Number of successful extensions: 205
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 188
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -