BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0034 (693 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 329 3e-91 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 183 2e-47 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 183 2e-47 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 169 2e-43 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 38 0.001 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 34 0.017 SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc... 30 0.36 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.36 SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl... 29 0.84 SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 28 1.1 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.9 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.6 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.4 SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar... 27 3.4 SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pomb... 26 4.5 SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 4.5 SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 5.9 SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 7.9 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 7.9 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 329 bits (808), Expect = 3e-91 Identities = 147/210 (70%), Positives = 171/210 (81%) Frame = +2 Query: 59 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 238 MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60 Query: 239 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 418 PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120 Query: 419 RKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVV 598 R+E+E+CD LQGFQ KIREEYPDR+M T+SV P+PK SDTVV Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180 Query: 599 EPYNATLSVHQLVENTDETYCIDNEALYDI 688 EPYNATLS+HQLVEN+DET+CIDNEAL I Sbjct: 181 EPYNATLSMHQLVENSDETFCIDNEALSSI 210 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 183 bits (446), Expect = 2e-47 Identities = 86/217 (39%), Positives = 128/217 (58%), Gaps = 6/217 (2%) Frame = +2 Query: 59 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 220 MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60 Query: 221 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 400 GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120 Query: 401 SVLDVVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPK 580 SVL+ +R+ +++C LQGF ++ EY + +SV P+P+ Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180 Query: 581 VSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDIC 691 VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC Sbjct: 181 VSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDIC 217 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 183 bits (446), Expect = 2e-47 Identities = 86/213 (40%), Positives = 125/213 (58%), Gaps = 2/213 (0%) Frame = +2 Query: 59 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 232 MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 233 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 412 YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120 Query: 413 VVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDT 592 +R+ +++C LQGF ++ EY + +SV P+P+VS + Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180 Query: 593 VVEPYNATLSVHQLVENTDETYCIDNEALYDIC 691 VVEPYN+ L+ H ++ D T+ +DNE+ YDIC Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDIC 213 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 169 bits (412), Expect = 2e-43 Identities = 79/212 (37%), Positives = 128/212 (60%), Gaps = 3/212 (1%) Frame = +2 Query: 62 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 241 REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62 Query: 242 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 415 RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+ Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121 Query: 416 VRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVP-SPKVSDT 592 + +E++ D L+GF ++ + YP +I+ TYSV P S VSD Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181 Query: 593 VVEPYNATLSVHQLVENTDETYCIDNEALYDI 688 VV+PYN+ L++ +L N D +DN AL I Sbjct: 182 VVQPYNSLLALKRLTLNADSVVVLDNAALAHI 213 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 38.3 bits (85), Expect = 0.001 Identities = 35/131 (26%), Positives = 66/131 (50%) Frame = +3 Query: 300 SDRSSVRTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLHIPSV 479 S S++ ++S S S P+T+ +T LSS + SS S+ + + +I +S+ S Sbjct: 225 SSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSS----SS 280 Query: 480 AAPGPVWAPSSSQRSVKSTPTES*THTQ*SPRPKYQTLSSNHTMRLSQFTS*LKTQTKPT 659 ++P + SS S S+PT + + T S + SS T+ S +S + PT Sbjct: 281 SSPTSTSSTISSSSSSSSSPTST-SSTISSSSSSSSSFSS--TLSSSSMSSSSSFSSSPT 337 Query: 660 ASTTRLYTTSA 692 +S++ + ++S+ Sbjct: 338 SSSSTISSSSS 348 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 34.3 bits (75), Expect = 0.017 Identities = 29/128 (22%), Positives = 63/128 (49%) Frame = +3 Query: 309 SSVRTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLHIPSVAAP 488 + V T+ S S +TT ++ SS T ++ S+ + + + + +S+ S ++ Sbjct: 120 NGVLQTTVSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSS 179 Query: 489 GPVWAPSSSQRSVKSTPTES*THTQ*SPRPKYQTLSSNHTMRLSQFTS*LKTQTKPTAST 668 + SSS S S+ + S + + S P + SS+H+ S +S + ++P++S+ Sbjct: 180 SSSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSSTSSSHSSSSSSSSS-SSSSSRPSSSS 238 Query: 669 TRLYTTSA 692 + + T S+ Sbjct: 239 SFITTMSS 246 >SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosaccharomyces pombe|chr 2|||Manual Length = 658 Score = 29.9 bits (64), Expect = 0.36 Identities = 29/105 (27%), Positives = 50/105 (47%) Frame = +3 Query: 363 PRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLHIPSVAAPGPVWAPSSSQRSVKSTPT 542 PR++T++ L S++ S S+ Q + +S L+ PS AP P + SSS T Sbjct: 219 PRESTEKPLLSVSALSS-SSHLQPTSATSSSSRLY-PSRPAPTPPASSSSSPLLSSQTVK 276 Query: 543 ES*THTQ*SPRPKYQTLSSNHTMRLSQFTS*LKTQTKPTASTTRL 677 + ++ P P + S+++ +R S L QT T+ T + Sbjct: 277 TTTSNASRQPSPLVSSKSTDNIIR-SHSPVLLTPQTLSTSETKHI 320 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.9 bits (64), Expect = 0.36 Identities = 35/124 (28%), Positives = 51/124 (41%), Gaps = 1/124 (0%) Frame = +3 Query: 321 TTSFSDSPAPAT-TGPRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLHIPSVAAPGPV 497 +TS + P P T T DT L + + S S ++ +S+L P + P Sbjct: 142 STSSTSIPIPPTSTSSTDTNSNPLPTTSTSCTTST---SIPPTGGSSSLSTP-ITPTVPP 197 Query: 498 WAPSSSQRSVKSTPTES*THTQ*SPRPKYQTLSSNHTMRLSQFTS*LKTQTKPTASTTRL 677 + SS+ + T T S T T SP P T + T + +S L T PT T Sbjct: 198 TSTSSTSIPIPPTSTSS-TDTNSSPLPTTSTSCTTSTSIPTGGSSSLSTPITPTVPPTST 256 Query: 678 YTTS 689 +TS Sbjct: 257 SSTS 260 >SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 709 Score = 28.7 bits (61), Expect = 0.84 Identities = 23/67 (34%), Positives = 33/67 (49%) Frame = +3 Query: 309 SSVRTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLHIPSVAAP 488 SS T+SFSD A + P+ T+ T S++ S + N ++ N PS P Sbjct: 132 SSPATSSFSDPKAFSAGVPKFTSD------TSSTVSSTPSLNHSL----QNSMPPSTPTP 181 Query: 489 GPVWAPS 509 PVWAP+ Sbjct: 182 PPVWAPT 188 >SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 28.3 bits (60), Expect = 1.1 Identities = 28/118 (23%), Positives = 53/118 (44%), Gaps = 1/118 (0%) Frame = +3 Query: 321 TTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLHIPSVAAPGPVW 500 TTS++ + + A++ ++ SSLT SS ++ + N + ++ + S +A Sbjct: 60 TTSYNYNTSSASSSSLTSSSAASSSLTSSSSLASSSTNSTTSASPTSSSLTSSSATSSSL 119 Query: 501 APSS-SQRSVKSTPTES*THTQ*SPRPKYQTLSSNHTMRLSQFTS*LKTQTKPTASTT 671 A SS + S+ S+ S + S SS + L+ ++ T PT+S T Sbjct: 120 ASSSTTSSSLASSSITSSSLASSSITSSSLASSSTTSSSLASSSTNSTTSATPTSSAT 177 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 27.5 bits (58), Expect = 1.9 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = +2 Query: 362 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 451 A+GH G ELV + D +RK+SE+ L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 27.1 bits (57), Expect = 2.6 Identities = 10/34 (29%), Positives = 22/34 (64%) Frame = +2 Query: 98 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 199 N++G E+++++ +DPT A + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 3.4 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +2 Query: 293 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 394 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1131 Score = 26.6 bits (56), Expect = 3.4 Identities = 14/41 (34%), Positives = 23/41 (56%) Frame = +3 Query: 300 SDRSSVRTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SA 422 S SS T+ S + + T P ++ V+SS++ SSM S+ Sbjct: 342 SSSSSASATATSSAESSIATSPITSSSNVVSSISTSSMDSS 382 >SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = +3 Query: 435 LAIAYRASNLHIPSVAAPGPVWAPSSSQRSVK 530 LA+ Y + + +V APG +W+P+ +R K Sbjct: 159 LAVRYGPLGIRV-NVCAPGTIWSPAWDERFKK 189 >SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/19 (57%), Positives = 15/19 (78%) Frame = +3 Query: 42 KITYTK*GKSFIYRPANVV 98 +I+YT G SFIYRP N++ Sbjct: 64 EISYT--GTSFIYRPGNII 80 >SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Schizosaccharomyces pombe|chr 1|||Manual Length = 1995 Score = 25.8 bits (54), Expect = 5.9 Identities = 26/97 (26%), Positives = 39/97 (40%), Gaps = 1/97 (1%) Frame = +3 Query: 318 RTTSFSDSPAPATTGPRDTTQRVLSSLTRSSM*SAKNQNLAIAYR-ASNLHIPSVAAPGP 494 R SF PA + PR S + M N ++ AY+ + L P++ A Sbjct: 627 RQYSFQPRPATPSNPPRSLPPP--SGQVNAPMSQTPNP-ISFAYQHGTPLATPTMRANSF 683 Query: 495 VWAPSSSQRSVKSTPTES*THTQ*SPRPKYQTLSSNH 605 P+SS ++ T + HT PK T +S H Sbjct: 684 NSYPASSAEPIRRPATTTVGHTPNLYSPKTNTYNSRH 720 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/44 (27%), Positives = 20/44 (45%) Frame = +2 Query: 314 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 445 RP +F G++ G + E D ++ + + ESCDC Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/32 (40%), Positives = 16/32 (50%) Frame = -1 Query: 351 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 256 P+ PK L R I P GPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,750,570 Number of Sequences: 5004 Number of extensions: 57162 Number of successful extensions: 205 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 188 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 197 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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