BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0028 (687 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g16250.1 68414.m01946 kelch repeat-containing F-box family pr... 29 2.2 At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 compone... 28 6.7 At4g17895.1 68417.m02667 ubiquitin-specific protease 20, putativ... 27 8.8 >At1g16250.1 68414.m01946 kelch repeat-containing F-box family protein contains Pfam profiles PF01344: Kelch motif, PF00646: F-box domain Length = 383 Score = 29.5 bits (63), Expect = 2.2 Identities = 14/36 (38%), Positives = 20/36 (55%) Frame = -1 Query: 351 SGLELNDAFKISSQNEQLAWAEWNSSNLYQKHDSTW 244 SGL F + S +Q+ +AE NSS ++ D TW Sbjct: 208 SGLSYRGCFHVLS--DQVGFAEQNSSEVFNPRDMTW 241 >At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 component, putative / oxoglutarate decarboxylase, putative / alpha-ketoglutaric dehydrogenase, putative similar to SP|P20967 2-oxoglutarate dehydrogenase E1 component, mitochondrial precursor (EC 1.2.4.2) (Alpha-ketoglutarate dehydrogenase) {Saccharomyces cerevisiae}; contains Pfam profiles PF02779: Transketolase, pyridine binding domain, PF00676: Dehydrogenase E1 component Length = 1025 Score = 27.9 bits (59), Expect = 6.7 Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 7/40 (17%) Frame = +2 Query: 569 LSIWRSP---DENFSLMSLRSLMT----SYC*TIAYRYYH 667 L +WR EN + +LRS+++ +YC TI Y Y H Sbjct: 173 LGVWRMSGFLSENRPVQTLRSILSRLEQAYCGTIGYEYMH 212 >At4g17895.1 68417.m02667 ubiquitin-specific protease 20, putative (UBP20) identical to ubiquitin-specific protease 20 GI:11993480 [Arabidopsis thaliana] Length = 695 Score = 27.5 bits (58), Expect = 8.8 Identities = 12/22 (54%), Positives = 14/22 (63%) Frame = -2 Query: 524 CMSHRTGFGGEALPRQVFSGRL 459 C S RT F G+ + VFSGRL Sbjct: 279 CGSDRTSFRGDITSQDVFSGRL 300 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,584,130 Number of Sequences: 28952 Number of extensions: 300069 Number of successful extensions: 637 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 627 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 637 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1457719448 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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