BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ceN-0010 (677 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g49160.1 68416.m05373 pyruvate kinase family protein similar ... 28 6.5 At5g46120.1 68418.m05672 hypothetical protein 27 8.6 At1g70630.1 68414.m08141 expressed protein 27 8.6 >At3g49160.1 68416.m05373 pyruvate kinase family protein similar to SP|Q92122 Pyruvate kinase, muscle isozyme (EC 2.7.1.40) (Cytosolic thyroid hormone binding protein) (CTHBP) {Xenopus laevis}; contains Pfam profile PF00224: Pyruvate kinase, barrel domain Length = 710 Score = 27.9 bits (59), Expect = 6.5 Identities = 15/38 (39%), Positives = 25/38 (65%) Frame = -3 Query: 216 DLEFMLSH*ASLGLTAPQHRLPN*ILYINASSSLLTAD 103 D+E + SH ASLGL++ + + + ++NAS +LL D Sbjct: 156 DIEQLNSHLASLGLSSLDNNNLDVLAHLNASINLLMND 193 >At5g46120.1 68418.m05672 hypothetical protein Length = 83 Score = 27.5 bits (58), Expect = 8.6 Identities = 12/28 (42%), Positives = 19/28 (67%) Frame = +2 Query: 194 WLNINSKSLQTEPPSLSSRLVQYLDRQI 277 WL N K +EPPS + +++++LDR I Sbjct: 30 WLERN-KRRHSEPPSTADQVIKFLDRII 56 >At1g70630.1 68414.m08141 expressed protein Length = 537 Score = 27.5 bits (58), Expect = 8.6 Identities = 13/38 (34%), Positives = 23/38 (60%) Frame = -2 Query: 214 FRVYVEPLSLPRSNSSTTSTPQLNIIYQRIELPAHCGL 101 FR + +SL RS S +S ++ + + I++P HCG+ Sbjct: 73 FRELQKMISL-RSLQSNSSASKMIMAWNNIDMPLHCGV 109 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,868,072 Number of Sequences: 28952 Number of extensions: 272674 Number of successful extensions: 568 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 562 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 568 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1428369392 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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