Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--2326
(631 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 249 2e-67
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 241 8e-65
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 134 8e-33
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 115 7e-27
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.2
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 5.2
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 6.8
SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 25 9.0
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 249 bits (610), Expect = 2e-67
Identities = 107/145 (73%), Positives = 125/145 (86%)
Frame = +3
Query: 69 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 248
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 249 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 428
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 429 RIRKLADQCTGLQGFLIFHSFGGGT 503
+IR++AD C+GLQGFL+FHSFGGGT
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGT 145
Score = 53.2 bits (122), Expect = 3e-08
Identities = 23/34 (67%), Positives = 29/34 (85%)
Frame = +1
Query: 505 GSGFTSLLMERLFVDYGKKSKLEFGIYPAPQVFT 606
GSGF +LL+ERL ++Y KKSKL+F +YPAPQV T
Sbjct: 146 GSGFGALLLERLAMEYTKKSKLQFSVYPAPQVST 179
Score = 25.4 bits (53), Expect = 6.8
Identities = 14/38 (36%), Positives = 18/38 (47%)
Frame = -3
Query: 545 TKRRSINKEVNPEPGTSTEGVEDQESLQTGTLVSELAD 432
TK+ + V P P ST VE S+ T +LAD
Sbjct: 162 TKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLAD 199
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 241 bits (589), Expect = 8e-65
Identities = 108/150 (72%), Positives = 127/150 (84%), Gaps = 5/150 (3%)
Frame = +3
Query: 69 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 233
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 234 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 413
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 414 DLVLDRIRKLADQCTGLQGFLIFHSFGGGT 503
D VL+RIR++AD C+GLQGFL+FHSFGGGT
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGT 149
Score = 51.6 bits (118), Expect = 9e-08
Identities = 22/34 (64%), Positives = 28/34 (82%)
Frame = +1
Query: 505 GSGFTSLLMERLFVDYGKKSKLEFGIYPAPQVFT 606
GSG +LL+ERL ++YGKKS L+F +YPAPQV T
Sbjct: 150 GSGLGALLLERLNMEYGKKSNLQFSVYPAPQVST 183
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 134 bits (325), Expect = 8e-33
Identities = 64/145 (44%), Positives = 86/145 (59%)
Frame = +3
Query: 69 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 248
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 249 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 428
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 429 RIRKLADQCTGLQGFLIFHSFGGGT 503
+R+ A+ C LQGF + HS GGGT
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGT 143
Score = 25.8 bits (54), Expect = 5.2
Identities = 10/31 (32%), Positives = 18/31 (58%)
Frame = +1
Query: 505 GSGFTSLLMERLFVDYGKKSKLEFGIYPAPQ 597
GSG +LL+ ++ +Y + F + PAP+
Sbjct: 144 GSGMGTLLLSKIREEYPDRMMATFSVAPAPK 174
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 115 bits (276), Expect = 7e-27
Identities = 58/146 (39%), Positives = 88/146 (60%), Gaps = 2/146 (1%)
Frame = +3
Query: 72 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 251
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 252 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 425
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 426 DRIRKLADQCTGLQGFLIFHSFGGGT 503
D I + AD L+GF + HS GGT
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGT 145
Score = 26.2 bits (55), Expect = 3.9
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +1
Query: 505 GSGFTSLLMERLFVDYGKKSKLEFGIYPAPQ 597
GSG S L+ERL Y KK + ++P Q
Sbjct: 146 GSGLGSFLLERLNDRYPKKIIQTYSVFPNSQ 176
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 5.2
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +1
Query: 148 STASSLMARCPQTRPSGVETILSTLSSARPELAST 252
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 5.2
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 167 WPDAHRQDHRGWRRFFQHFLQR 232
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 6.8
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +1
Query: 58 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 180
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 400
Score = 25.0 bits (52), Expect = 9.0
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -3
Query: 122 ITDLDSGLANVYRDALTHFDLSF 54
+T +D GLA YRD TH + +
Sbjct: 146 VTMIDFGLAKKYRDFKTHVHIPY 168
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,783,130
Number of Sequences: 5004
Number of extensions: 60474
Number of successful extensions: 185
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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