Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ce--2169
         (730 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces pomb...   104   1e-23
SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces pomb...    87   2e-18
SPBC119.13c |prp31||U4/U6 x U5 tri-snRNP complex subunit Prp31|S...    36   0.004
SPAC1952.02 |||ribosome biogenesis protein|Schizosaccharomyces p...    34   0.024
SPAC3A12.13c |||translation initiation factor eIF3 complex subun...    29   0.51 
SPAC23H4.01c ||SPAP27G11.01|sterol binding ankyrin repeat protei...    27   2.7  
SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynei...    27   2.7  
SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint compone...    27   3.6  
SPBP8B7.04 |mug45||sequence orphan|Schizosaccharomyces pombe|chr...    27   3.6  
SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription te...    26   4.8  
SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos...    26   6.3  
SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe...    26   6.3  
SPCC188.07 |ccq1||telomere maintenence protein|Schizosaccharomyc...    25   8.4  
SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    25   8.4  

>SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 508

 Score =  104 bits (249), Expect = 1e-23
 Identities = 70/180 (38%), Positives = 91/180 (50%), Gaps = 14/180 (7%)
 Frame = -3

Query: 644 TIQIFGAKKALFRALKTKKDTPKYGLIYHAQLVGQCSTKNKGKMSRMLAAKAALATRVDA 465
           TIQI GA+KALFRALKTK  TPKYGLIYHA LVGQ ++KNKGK++R+LA KAAL+ RVDA
Sbjct: 316 TIQILGAEKALFRALKTKHSTPKYGLIYHASLVGQANSKNKGKIARVLATKAALSLRVDA 375

Query: 464 FG--EDVSFELGAEHKV------------KXXXXXXXXXXXXXXRISGTGKAKIKFEKYH 327
               +  +  +G E+++            K              +I+GT       +   
Sbjct: 376 LSDKDTTNGNIGLENRIRVENRLRSLEGGKLLPLPTAPVQQSKVQINGTSAYSTATDAVT 435

Query: 326 SKSEVYQYPDASDNTIDKKPIKREHSPDEDQVSAKKIKLENDVSKVSLKNIKTENEEPAE 147
             +E  Q     D  I+KK  K     + D  S+KK K E    K      K+E  E  E
Sbjct: 436 KDAEESQEDVEMDIVIEKKKKKSSKLKEADGESSKKEKKEKKDKKHKKSKRKSEESEDGE 495



 Score = 29.9 bits (64), Expect = 0.39
 Identities = 13/21 (61%), Positives = 16/21 (76%)
 Frame = -1

Query: 721 IGEHIGARLIAHAGSPNEFSK 659
           +GE +GARLIAHAGS    +K
Sbjct: 291 VGELVGARLIAHAGSLMNLAK 311


>SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 497

 Score = 87.0 bits (206), Expect = 2e-18
 Identities = 36/63 (57%), Positives = 51/63 (80%)
 Frame = -3

Query: 644 TIQIFGAKKALFRALKTKKDTPKYGLIYHAQLVGQCSTKNKGKMSRMLAAKAALATRVDA 465
           T+QI GA+KALFRALKT+ +TPKYG+IYH+  +G+   KNKG++SR LA K ++A+R+D 
Sbjct: 328 TVQILGAEKALFRALKTRGNTPKYGIIYHSSFIGKAGAKNKGRISRFLANKCSIASRIDN 387

Query: 464 FGE 456
           F +
Sbjct: 388 FSD 390



 Score = 31.9 bits (69), Expect = 0.096
 Identities = 17/37 (45%), Positives = 20/37 (54%)
 Frame = -1

Query: 721 IGEHIGARLIAHAGSPNEFSKTSCFLQYRFLVQKKLF 611
           IGE +GARLI+HAGS    SK           +K LF
Sbjct: 303 IGEMVGARLISHAGSLTNLSKCPASTVQILGAEKALF 339


>SPBC119.13c |prp31||U4/U6 x U5 tri-snRNP complex subunit
           Prp31|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 518

 Score = 36.3 bits (80), Expect = 0.004
 Identities = 20/55 (36%), Positives = 32/55 (58%), Gaps = 2/55 (3%)
 Frame = -3

Query: 575 YGLIYHAQLVGQCSTKNKGKMSRMLAAKAALATRVDAFGE--DVSFELGAEHKVK 417
           YG +Y +++V +     + +  RM AAK ALA R+D+  E  D SF + A  +V+
Sbjct: 295 YGFLYMSEIVQKTPPDVRKQAIRMTAAKVALAARIDSIHEYPDGSFGISARKEVE 349


>SPAC1952.02 |||ribosome biogenesis protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 202

 Score = 33.9 bits (74), Expect = 0.024
 Identities = 19/73 (26%), Positives = 36/73 (49%), Gaps = 4/73 (5%)
 Frame = -3

Query: 350 KIKFEKYHSK----SEVYQYPDASDNTIDKKPIKREHSPDEDQVSAKKIKLENDVSKVSL 183
           K++  KYHSK    S V++Y      T ++  + +        VS+KK+K+     K S 
Sbjct: 80  KLRMAKYHSKYSALSSVFRYAGRLCGTFEEDLVDKSLDSSVSPVSSKKVKVRKTSGKESS 139

Query: 182 KNIKTENEEPAED 144
           K  K++ ++  ++
Sbjct: 140 KREKSKKKKEKKE 152


>SPAC3A12.13c |||translation initiation factor eIF3 complex
           subunit|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 274

 Score = 29.5 bits (63), Expect = 0.51
 Identities = 14/63 (22%), Positives = 27/63 (42%)
 Frame = -3

Query: 326 SKSEVYQYPDASDNTIDKKPIKREHSPDEDQVSAKKIKLENDVSKVSLKNIKTENEEPAE 147
           +K   + +P   D+    KP KR    +++     K  L+ND   VS K+  +   +   
Sbjct: 13  AKPAEFDFPLGKDSQSSSKPKKRFDDEEDEDEEENKESLQNDSHSVSQKSSSSSQNDQGS 72

Query: 146 DAL 138
           + +
Sbjct: 73  NKM 75


>SPAC23H4.01c ||SPAP27G11.01|sterol binding ankyrin repeat
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 945

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 12/38 (31%), Positives = 21/38 (55%), Gaps = 2/38 (5%)
 Frame = -3

Query: 356 KAKIKFEKYHSKSEVYQYPDASDNTIDKKPI--KREHS 249
           +  IKF+ YH K++     D S+   D+  +  KR+H+
Sbjct: 32  RKSIKFDIYHKKNDTSSLLDGSNKNTDRSILHTKRQHT 69


>SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynein
           Mcp5/Num1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 968

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 15/62 (24%), Positives = 27/62 (43%)
 Frame = -3

Query: 368 SGTGKAKIKFEKYHSKSEVYQYPDASDNTIDKKPIKREHSPDEDQVSAKKIKLENDVSKV 189
           +GT     K    H+  E   YP A  NT      + E +  +   SA+++K  +  + +
Sbjct: 736 NGTSSMLFKDFTKHNNWEASTYPSAPKNTRTYPTAEVEKTRHDSSQSARQLKARSTATTI 795

Query: 188 SL 183
           S+
Sbjct: 796 SI 797


>SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint component
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 809

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 16/44 (36%), Positives = 24/44 (54%)
 Frame = -3

Query: 278 DKKPIKREHSPDEDQVSAKKIKLENDVSKVSLKNIKTENEEPAE 147
           D  P   +HS   D+    K+  +ND +  SL+++K ENEE  E
Sbjct: 622 DPAPQPAKHSR-VDRYEHPKVLEDNDSTISSLESLKRENEELRE 664


>SPBP8B7.04 |mug45||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 819

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 21/64 (32%), Positives = 29/64 (45%)
 Frame = -3

Query: 329 HSKSEVYQYPDASDNTIDKKPIKREHSPDEDQVSAKKIKLENDVSKVSLKNIKTENEEPA 150
           HS S   + P    NT     +K E S  +  +S   I  +    KVS++NIK E   P 
Sbjct: 237 HSSS-TSELPCTYGNTSSIFQVKNEMSNIKSAISEGYIAHDQQSKKVSVQNIKKEFLIPF 295

Query: 149 EDAL 138
           E +L
Sbjct: 296 EFSL 299


>SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription
           termination factor Reb1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 504

 Score = 26.2 bits (55), Expect = 4.8
 Identities = 17/61 (27%), Positives = 25/61 (40%)
 Frame = -2

Query: 726 TSLVNTLEHDLLLMQAHLMNLAKHPAFYNTDFWCKKSSFPCLKNEERHTKVWIDISRSVS 547
           ++L   L H       H +  A +P F +   W K+      KN   H K W  I R ++
Sbjct: 285 SNLYKKLSHRDAKSIYHHVRRAYNP-FEDRCVWSKEEDEELRKNVVEHGKCWTKIGRKMA 343

Query: 546 R 544
           R
Sbjct: 344 R 344


>SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase
           Ppk29|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 872

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 18/56 (32%), Positives = 29/56 (51%), Gaps = 5/56 (8%)
 Frame = -3

Query: 359 GKAKIKFEKYHSK---SEVY--QYPDASDNTIDKKPIKREHSPDEDQVSAKKIKLE 207
           GK  +  EKY ++   S VY  Q    +D +     +KR +SPDE+ +   K ++E
Sbjct: 21  GKYNVTIEKYIAEGGFSHVYLVQTNSKTDGSPITAVLKRMYSPDENALRFVKTEIE 76


>SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 969

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 12/35 (34%), Positives = 21/35 (60%)
 Frame = -3

Query: 248 PDEDQVSAKKIKLENDVSKVSLKNIKTENEEPAED 144
           P+ D    + IKLEND+  + +++ +T+N   A D
Sbjct: 17  PNLDDREYRLIKLENDLEVLLVRDPETDNASAAID 51


>SPCC188.07 |ccq1||telomere maintenence protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 735

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 10/21 (47%), Positives = 15/21 (71%)
 Frame = -2

Query: 696 LLLMQAHLMNLAKHPAFYNTD 634
           L +  AH  N+AKHP+ Y+T+
Sbjct: 177 LFIQSAH--NIAKHPSLYDTE 195


>SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 547

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 15/56 (26%), Positives = 25/56 (44%)
 Frame = -2

Query: 720 LVNTLEHDLLLMQAHLMNLAKHPAFYNTDFWCKKSSFPCLKNEERHTKVWIDISRS 553
           LV+ LE   L  ++HL N+++  +  N +   K        N     +  +DIS S
Sbjct: 365 LVSQLESQRLYYESHLSNMSQKLSRVNEELVLKTKIATASSNANTDLRSRVDISES 420


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,355,623
Number of Sequences: 5004
Number of extensions: 46487
Number of successful extensions: 163
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)