Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--1976
(714 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 266 2e-72
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 261 6e-71
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 139 4e-34
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 94 2e-20
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 30 0.29
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.1
SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosacch... 26 6.1
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.1
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 266 bits (653), Expect = 2e-72
Identities = 118/166 (71%), Positives = 142/166 (85%)
Frame = +1
Query: 202 PKGQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXX 381
P LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH
Sbjct: 86 PYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSF 145
Query: 382 XXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCA 561
LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC
Sbjct: 146 GGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCT 205
Query: 562 FMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGA 699
FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSITASLRF G+
Sbjct: 206 FMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGS 251
Score = 95.9 bits (228), Expect = 5e-21
Identities = 45/72 (62%), Positives = 53/72 (73%), Gaps = 5/72 (6%)
Frame = +2
Query: 5 NACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEP 169
NACWELYCLEHGI PDG PT+ K +D F TFFSETG GK VPR+++VDLEP
Sbjct: 18 NACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLEP 76
Query: 170 TVVDEVRTGTYQ 205
V+D+VRTG Y+
Sbjct: 77 NVIDQVRTGPYK 88
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 261 bits (640), Expect = 6e-71
Identities = 117/166 (70%), Positives = 141/166 (84%)
Frame = +1
Query: 202 PKGQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXX 381
P LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH
Sbjct: 82 PYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSF 141
Query: 382 XXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCA 561
LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC
Sbjct: 142 GGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCT 201
Query: 562 FMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGA 699
FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASLRF+G+
Sbjct: 202 FMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGS 247
Score = 105 bits (251), Expect = 8e-24
Identities = 43/67 (64%), Positives = 53/67 (79%)
Frame = +2
Query: 5 NACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDE 184
NACWELYCLEHGIQP+G M + D F+TFFSETG GK+VPR+++VDLEP V+D+
Sbjct: 18 NACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQ 77
Query: 185 VRTGTYQ 205
VRTG Y+
Sbjct: 78 VRTGPYR 84
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 139 bits (336), Expect = 4e-34
Identities = 64/163 (39%), Positives = 96/163 (58%)
Frame = +1
Query: 208 GQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXX 387
G LF P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C LQGF + H
Sbjct: 82 GNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGG 141
Query: 388 XXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFM 567
LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F
Sbjct: 142 GTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFC 201
Query: 568 VDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDG 696
+DNEA+ I L I+ P+Y +LN L+ +++ +T S RF G
Sbjct: 202 IDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPG 244
Score = 49.6 bits (113), Expect = 4e-07
Identities = 25/65 (38%), Positives = 34/65 (52%)
Frame = +2
Query: 8 ACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEV 187
A W EHG+ G T + N +F+E GK+VPRAV VDLEP +D V
Sbjct: 19 AFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAV 76
Query: 188 RTGTY 202
++G +
Sbjct: 77 KSGKF 81
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 94.3 bits (224), Expect = 2e-20
Identities = 53/166 (31%), Positives = 88/166 (53%), Gaps = 3/166 (1%)
Frame = +1
Query: 208 GQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXX 381
G L++PE ++ K A NN+A G Y+ + I + ++D I + AD L+GF + H
Sbjct: 83 GSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSI 141
Query: 382 XXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDC 558
L+ERL+ Y KK ++++P Q VS VV+PYNS+L ++D
Sbjct: 142 AGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADS 201
Query: 559 AFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDG 696
++DN A+ I L + PT+ N+L+ ++S+ T +LR+ G
Sbjct: 202 VVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPG 247
Score = 60.5 bits (140), Expect = 2e-10
Identities = 29/78 (37%), Positives = 47/78 (60%)
Frame = +2
Query: 14 WELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRT 193
W+ CLEHGI PDG + + T G D + FF ++ +++PRA+ +DLEP VV+ + +
Sbjct: 22 WQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILS 79
Query: 194 GTYQRDSCFIQNNLLLVR 247
TY S + N+L+ +
Sbjct: 80 DTY--GSLYNPENILITK 95
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 30.3 bits (65), Expect = 0.29
Identities = 15/37 (40%), Positives = 23/37 (62%)
Frame = -1
Query: 690 ESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 580
+ Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 182 DKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 6.1
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 33 STASSLMARCPQTRPSGVETILSTLSSARPELAST 137
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPBC12C2.05c |||diacylglycerol binding protein Bzz1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 25.8 bits (54), Expect = 6.1
Identities = 25/86 (29%), Positives = 39/86 (45%), Gaps = 8/86 (9%)
Frame = -1
Query: 699 SAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADV---IDGFIV-----DHESTVRVL 544
+A ES R++ D S Q + E T A+ +DG ++ +HE V+
Sbjct: 478 NAYESYRNTYTDDMDSSSIYQTSLSNVKTEETTPAEPASKVDGVVLYDFTGEHEG---VI 534
Query: 543 KGCVGGEDRVVGLDDGSGNLRRGVDG 466
G E ++ DDGSG +R +DG
Sbjct: 535 TASEGQEFTLLEPDDGSGWVRVKIDG 560
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 52 WPDAHRQDHRGWRRFFQHFLQR 117
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,911,962
Number of Sequences: 5004
Number of extensions: 59312
Number of successful extensions: 222
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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