Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--1928
(699 letters)
Database: celegans
27,780 sequences; 12,740,198 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Z29443-8|CAE45742.1| 455|Caenorhabditis elegans Hypothetical pr... 132 2e-31
Z29443-7|CAA82571.2| 497|Caenorhabditis elegans Hypothetical pr... 132 2e-31
U40483-1|AAA99775.1| 322|Caenorhabditis elegans NEX1 annexin pr... 105 3e-23
U00064-7|AAB52702.1| 322|Caenorhabditis elegans Annexin family ... 105 3e-23
Z32680-3|CAA83598.1| 317|Caenorhabditis elegans Hypothetical pr... 88 5e-18
U88315-15|AAB42365.1| 351|Caenorhabditis elegans Annexin family... 64 1e-10
U40799-3|AAA81481.1| 323|Caenorhabditis elegans Hypothetical pr... 28 7.4
AF003134-5|AAB54139.1| 357|Caenorhabditis elegans Nek (never in... 28 7.4
U21321-3|AAG00052.2| 655|Caenorhabditis elegans Hypothetical pr... 27 9.8
>Z29443-8|CAE45742.1| 455|Caenorhabditis elegans Hypothetical
protein T07C4.9b protein.
Length = 455
Score = 132 bits (319), Expect = 2e-31
Identities = 66/148 (44%), Positives = 93/148 (62%)
Frame = +3
Query: 117 TPTVYXXXXXXXXXXXXTLRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGKDL 296
TP+V+ LRKAMKG G + +I +LC+R QR EI++ FK YGKDL
Sbjct: 140 TPSVFPVQGFNSNADAEVLRKAMKGLGCNNSKVISILCQRTNWQRQEISKAFKVMYGKDL 199
Query: 297 ISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTI 476
I ELK EL G+ E++I+ALM + AK+LH A+ G+GT E +IEI+ + +N I+ +
Sbjct: 200 IKELKGELHGDFEDLILALMDAPAIYDAKQLHRAMEGLGTKESVLIEIMTSRTNAQIQQV 259
Query: 477 SAFYEQLYGKSLESDLKGDTSGHFKEIV 560
Y+ L+ K LE DL G+TSGHFK ++
Sbjct: 260 RDAYKMLFKKDLERDLIGETSGHFKRLL 287
Score = 73.7 bits (173), Expect = 1e-13
Identities = 39/139 (28%), Positives = 74/139 (53%), Gaps = 3/139 (2%)
Frame = +3
Query: 171 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGKDLISELKSELTGNLEN---V 341
L+ K GTDE +L + Q + E ++ + ++ E +G++ +
Sbjct: 314 LQAGEKRLGTDESTFNAILASQNFSQLRLVFEEYQKASNHSIEKAIEFEFSGDIRDGLLA 373
Query: 342 IVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESD 521
++A++ P ++AK LHD++ G+GT + +I + T + Y + I ++ LY SLE+
Sbjct: 374 VIAVIRNRPAYFAKLLHDSMKGLGTRDNDLIRLCVTRAEYDMGDIRNMFQSLYRTSLENM 433
Query: 522 LKGDTSGHFKEIVRVVVHG 578
+KGD SG +KE + +V+G
Sbjct: 434 IKGDCSGAYKEGLIALVNG 452
Score = 47.2 bits (107), Expect = 1e-05
Identities = 34/146 (23%), Positives = 69/146 (47%), Gaps = 12/146 (8%)
Frame = +3
Query: 171 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGKDLISELKSELTGNLENVIVA 350
L +AM+G GT E +I+++ R Q ++ + +K + KDL +L E +G+ + ++V+
Sbjct: 230 LHRAMEGLGTKESVLIEIMTSRTNAQIQQVRDAYKMLFKKDLERDLIGETSGHFKRLLVS 289
Query: 351 L-------MTPLPHFYAKE-----LHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQ 494
L + A + L +GTDE IL + + +R + Y++
Sbjct: 290 LCAGGRDESSQTDGLRANQDARRLLQAGEKRLGTDESTFNAILASQNFSQLRLVFEEYQK 349
Query: 495 LYGKSLESDLKGDTSGHFKEIVRVVV 572
S+E ++ + SG ++ + V+
Sbjct: 350 ASNHSIEKAIEFEFSGDIRDGLLAVI 375
Score = 28.3 bits (60), Expect = 5.6
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = +1
Query: 550 KRLCVSLCMANRDENQGIDEGSTKADAEALARRW*RSMG 666
KRL VSLC RDE+ D DA L + + +G
Sbjct: 284 KRLLVSLCAGGRDESSQTDGLRANQDARRLLQAGEKRLG 322
>Z29443-7|CAA82571.2| 497|Caenorhabditis elegans Hypothetical
protein T07C4.9a protein.
Length = 497
Score = 132 bits (319), Expect = 2e-31
Identities = 66/148 (44%), Positives = 93/148 (62%)
Frame = +3
Query: 117 TPTVYXXXXXXXXXXXXTLRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGKDL 296
TP+V+ LRKAMKG G + +I +LC+R QR EI++ FK YGKDL
Sbjct: 182 TPSVFPVQGFNSNADAEVLRKAMKGLGCNNSKVISILCQRTNWQRQEISKAFKVMYGKDL 241
Query: 297 ISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTI 476
I ELK EL G+ E++I+ALM + AK+LH A+ G+GT E +IEI+ + +N I+ +
Sbjct: 242 IKELKGELHGDFEDLILALMDAPAIYDAKQLHRAMEGLGTKESVLIEIMTSRTNAQIQQV 301
Query: 477 SAFYEQLYGKSLESDLKGDTSGHFKEIV 560
Y+ L+ K LE DL G+TSGHFK ++
Sbjct: 302 RDAYKMLFKKDLERDLIGETSGHFKRLL 329
Score = 73.7 bits (173), Expect = 1e-13
Identities = 39/139 (28%), Positives = 74/139 (53%), Gaps = 3/139 (2%)
Frame = +3
Query: 171 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGKDLISELKSELTGNLEN---V 341
L+ K GTDE +L + Q + E ++ + ++ E +G++ +
Sbjct: 356 LQAGEKRLGTDESTFNAILASQNFSQLRLVFEEYQKASNHSIEKAIEFEFSGDIRDGLLA 415
Query: 342 IVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESD 521
++A++ P ++AK LHD++ G+GT + +I + T + Y + I ++ LY SLE+
Sbjct: 416 VIAVIRNRPAYFAKLLHDSMKGLGTRDNDLIRLCVTRAEYDMGDIRNMFQSLYRTSLENM 475
Query: 522 LKGDTSGHFKEIVRVVVHG 578
+KGD SG +KE + +V+G
Sbjct: 476 IKGDCSGAYKEGLIALVNG 494
Score = 47.2 bits (107), Expect = 1e-05
Identities = 34/146 (23%), Positives = 69/146 (47%), Gaps = 12/146 (8%)
Frame = +3
Query: 171 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGKDLISELKSELTGNLENVIVA 350
L +AM+G GT E +I+++ R Q ++ + +K + KDL +L E +G+ + ++V+
Sbjct: 272 LHRAMEGLGTKESVLIEIMTSRTNAQIQQVRDAYKMLFKKDLERDLIGETSGHFKRLLVS 331
Query: 351 L-------MTPLPHFYAKE-----LHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQ 494
L + A + L +GTDE IL + + +R + Y++
Sbjct: 332 LCAGGRDESSQTDGLRANQDARRLLQAGEKRLGTDESTFNAILASQNFSQLRLVFEEYQK 391
Query: 495 LYGKSLESDLKGDTSGHFKEIVRVVV 572
S+E ++ + SG ++ + V+
Sbjct: 392 ASNHSIEKAIEFEFSGDIRDGLLAVI 417
Score = 28.3 bits (60), Expect = 5.6
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = +1
Query: 550 KRLCVSLCMANRDENQGIDEGSTKADAEALARRW*RSMG 666
KRL VSLC RDE+ D DA L + + +G
Sbjct: 326 KRLLVSLCAGGRDESSQTDGLRANQDARRLLQAGEKRLG 364
>U40483-1|AAA99775.1| 322|Caenorhabditis elegans NEX1 annexin
protein.
Length = 322
Score = 105 bits (252), Expect = 3e-23
Identities = 53/127 (41%), Positives = 83/127 (65%), Gaps = 1/127 (0%)
Frame = +3
Query: 201 DEKAIIDVLCRRGIVQRLEIAETFKTNYGKDLISELKSELTGNLENVIVALM-TPLPHFY 377
++ A+++V+ QR ++ E +K YGKD+I L + +G+LE I ALM TPL +
Sbjct: 31 EKDAVVNVITSISNAQRQQLREPYKLKYGKDIIQALDKKFSGDLEKAIFALMETPLD-YD 89
Query: 378 AKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTSGHFKEI 557
K+L A+ G+GTDE +IEILC+ + +R I YE+ YGK+LE+D+ GDTSG F+++
Sbjct: 90 VKQLKAAMKGLGTDEAVLIEILCSRTVDQLRAIRVTYEKEYGKALEADIAGDTSGEFRDL 149
Query: 558 VRVVVHG 578
+ +V G
Sbjct: 150 LVSLVTG 156
Score = 68.1 bits (159), Expect = 6e-12
Identities = 34/129 (26%), Positives = 69/129 (53%), Gaps = 3/129 (2%)
Frame = +3
Query: 201 DEKAIIDVLCRRGIVQRLEIAETFKTNYGKDLISELKSELTGNLEN---VIVALMTPLPH 371
D + +L + Q ++ F+ G + ++ E +G+L+ IV +
Sbjct: 188 DGTHFLHILATQNQYQLRKVFAYFQELAGGSIEKSIEKEFSGDLQKSYLTIVRAASDKQK 247
Query: 372 FYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTSGHFK 551
F+A++LH ++ G+GT + +I ++ T S + I A +++LY KSL +KGDTSG ++
Sbjct: 248 FFAQQLHASMKGLGTRDNDLIRVIVTRSEVDLELIKAEFQELYSKSLADTVKGDTSGAYR 307
Query: 552 EIVRVVVHG 578
+ + +++G
Sbjct: 308 DALLSIING 316
Score = 67.7 bits (158), Expect = 7e-12
Identities = 43/151 (28%), Positives = 76/151 (50%), Gaps = 13/151 (8%)
Frame = +3
Query: 171 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGKDLISELKSELTGNLENVIVA 350
L+ AMKG GTDE +I++LC R + Q I T++ YGK L +++ + +G +++V+
Sbjct: 93 LKAAMKGLGTDEAVLIEILCSRTVDQLRAIRVTYEKEYGKALEADIAGDTSGEFRDLLVS 152
Query: 351 LMTPLPHFY-----AKELHDAVSGIG--------TDEEAIIEILCTLSNYGIRTISAFYE 491
L+T A+ DAV D + IL T + Y +R + A+++
Sbjct: 153 LVTGSKDGSHDTNDAQAKDDAVRLFADGKAKLAKKDGTHFLHILATQNQYQLRKVFAYFQ 212
Query: 492 QLYGKSLESDLKGDTSGHFKEIVRVVVHGQS 584
+L G S+E ++ + SG ++ +V S
Sbjct: 213 ELAGGSIEKSIEKEFSGDLQKSYLTIVRAAS 243
Score = 33.1 bits (72), Expect = 0.20
Identities = 17/62 (27%), Positives = 31/62 (50%)
Frame = +3
Query: 171 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGKDLISELKSELTGNLENVIVA 350
L +MKG GT + +I V+ R V I F+ Y K L +K + +G + +++
Sbjct: 253 LHASMKGLGTRDNDLIRVIVTRSEVDLELIKAEFQELYSKSLADTVKGDTSGAYRDALLS 312
Query: 351 LM 356
++
Sbjct: 313 II 314
>U00064-7|AAB52702.1| 322|Caenorhabditis elegans Annexin family
protein 1 protein.
Length = 322
Score = 105 bits (252), Expect = 3e-23
Identities = 53/127 (41%), Positives = 83/127 (65%), Gaps = 1/127 (0%)
Frame = +3
Query: 201 DEKAIIDVLCRRGIVQRLEIAETFKTNYGKDLISELKSELTGNLENVIVALM-TPLPHFY 377
++ A+++V+ QR ++ E +K YGKD+I L + +G+LE I ALM TPL +
Sbjct: 31 EKDAVVNVITSISNAQRQQLREPYKLKYGKDIIQALDKKFSGDLEKAIFALMETPLD-YD 89
Query: 378 AKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTSGHFKEI 557
K+L A+ G+GTDE +IEILC+ + +R I YE+ YGK+LE+D+ GDTSG F+++
Sbjct: 90 VKQLKAAMKGLGTDEAVLIEILCSRTVDQLRAIRVTYEKEYGKALEADIAGDTSGEFRDL 149
Query: 558 VRVVVHG 578
+ +V G
Sbjct: 150 LVSLVTG 156
Score = 68.1 bits (159), Expect = 6e-12
Identities = 34/129 (26%), Positives = 69/129 (53%), Gaps = 3/129 (2%)
Frame = +3
Query: 201 DEKAIIDVLCRRGIVQRLEIAETFKTNYGKDLISELKSELTGNLEN---VIVALMTPLPH 371
D + +L + Q ++ F+ G + ++ E +G+L+ IV +
Sbjct: 188 DGTHFLHILATQNQYQLRKVFAYFQELAGGSIEKSIEKEFSGDLQKSYLTIVRAASDKQK 247
Query: 372 FYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTSGHFK 551
F+A++LH ++ G+GT + +I ++ T S + I A +++LY KSL +KGDTSG ++
Sbjct: 248 FFAQQLHASMKGLGTRDNDLIRVIVTRSEVDLELIKAEFQELYSKSLADTVKGDTSGAYR 307
Query: 552 EIVRVVVHG 578
+ + +++G
Sbjct: 308 DALLSIING 316
Score = 67.7 bits (158), Expect = 7e-12
Identities = 43/151 (28%), Positives = 76/151 (50%), Gaps = 13/151 (8%)
Frame = +3
Query: 171 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGKDLISELKSELTGNLENVIVA 350
L+ AMKG GTDE +I++LC R + Q I T++ YGK L +++ + +G +++V+
Sbjct: 93 LKAAMKGLGTDEAVLIEILCSRTVDQLRAIRVTYEKEYGKALEADIAGDTSGEFRDLLVS 152
Query: 351 LMTPLPHFY-----AKELHDAVSGIG--------TDEEAIIEILCTLSNYGIRTISAFYE 491
L+T A+ DAV D + IL T + Y +R + A+++
Sbjct: 153 LVTGSKDGSHDTNDAQAKDDAVRLFADGKAKLAKKDGTHFLHILATQNQYQLRKVFAYFQ 212
Query: 492 QLYGKSLESDLKGDTSGHFKEIVRVVVHGQS 584
+L G S+E ++ + SG ++ +V S
Sbjct: 213 ELAGGSIEKSIEKEFSGDLQKSYLTIVRAAS 243
Score = 33.1 bits (72), Expect = 0.20
Identities = 17/62 (27%), Positives = 31/62 (50%)
Frame = +3
Query: 171 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGKDLISELKSELTGNLENVIVA 350
L +MKG GT + +I V+ R V I F+ Y K L +K + +G + +++
Sbjct: 253 LHASMKGLGTRDNDLIRVIVTRSEVDLELIKAEFQELYSKSLADTVKGDTSGAYRDALLS 312
Query: 351 LM 356
++
Sbjct: 313 II 314
>Z32680-3|CAA83598.1| 317|Caenorhabditis elegans Hypothetical
protein C28A5.3 protein.
Length = 317
Score = 88.2 bits (209), Expect = 5e-18
Identities = 48/137 (35%), Positives = 76/137 (55%)
Frame = +3
Query: 171 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGKDLISELKSELTGNLENVIVA 350
L KA++ ++ I+++L QR I +KT YGKDL E+K +G+ E+ +VA
Sbjct: 21 LEKALRN--KEKLKILEILVSVNNTQRQMIRTPYKTRYGKDLEDEIKKAFSGDFEDFLVA 78
Query: 351 LMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKG 530
L+ EL+ +V G+GT+E+ +IEIL T +N I Y Y KSLE +
Sbjct: 79 LLQTPTKLDVTELNRSVKGLGTNEKNLIEILTTRTNEEIEAAKNTYFMTYSKSLEDAISA 138
Query: 531 DTSGHFKEIVRVVVHGQ 581
DTSG F+ ++ V++ +
Sbjct: 139 DTSGDFRRLLIVILQAK 155
Score = 54.8 bits (126), Expect = 6e-08
Identities = 28/114 (24%), Positives = 61/114 (53%), Gaps = 3/114 (2%)
Frame = +3
Query: 255 EIAETFKTNYGKDLISELKSELTGNLENVIVALMTPL---PHFYAKELHDAVSGIGTDEE 425
++ E + GK+ + EL+G+ +N++++L+ P ++A +H A G+GT ++
Sbjct: 203 KVIEEVERQSGKEFQKLIDKELSGDFKNLVLSLIETSRNKPRYFANSIHLATKGMGTRDK 262
Query: 426 AIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTSGHFKEIVRVVVHGQSR 587
+I IL + S + I ++ L+GK L +K + +++ + ++V G R
Sbjct: 263 DLIRILVSRSENDLVIIEHEFQTLFGKPLTQLIKEECKAEYRDGLTMLVKGNPR 316
Score = 32.7 bits (71), Expect = 0.26
Identities = 19/67 (28%), Positives = 31/67 (46%)
Frame = +3
Query: 168 TLRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGKDLISELKSELTGNLENVIV 347
++ A KG GT +K +I +L R + I F+T +GK L +K E + +
Sbjct: 249 SIHLATKGMGTRDKDLIRILVSRSENDLVIIEHEFQTLFGKPLTQLIKEECKAEYRDGLT 308
Query: 348 ALMTPLP 368
L+ P
Sbjct: 309 MLVKGNP 315
>U88315-15|AAB42365.1| 351|Caenorhabditis elegans Annexin family
protein 4 protein.
Length = 351
Score = 63.7 bits (148), Expect = 1e-10
Identities = 37/125 (29%), Positives = 62/125 (49%)
Frame = +3
Query: 168 TLRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGKDLISELKSELTGNLENVIV 347
TLR ++ G T ++ II+ + QR +I ++ Y + L+ +L+ E G +
Sbjct: 33 TLRSSLHGLETKDQTIINTVLYHNNFQRQKILGAYEDMYSRKLLEDLEEECGGFFFEMCQ 92
Query: 348 ALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLK 527
AL P P++ A+ ++ ++S D IEI CT S +R + Y+ Y KSL+ DL
Sbjct: 93 ALFKPAPNYDAQCVYKSLSNRHGDRSVAIEIACTRSPRQMRALRDTYQIDYRKSLDKDLT 152
Query: 528 GDTSG 542
G
Sbjct: 153 VKVEG 157
>U40799-3|AAA81481.1| 323|Caenorhabditis elegans Hypothetical
protein F42C5.5 protein.
Length = 323
Score = 27.9 bits (59), Expect = 7.4
Identities = 21/78 (26%), Positives = 36/78 (46%)
Frame = +3
Query: 261 AETFKTNYGKDLISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEI 440
A TF+T YG LIS+ ++ T N +++ + + + L + G +E E
Sbjct: 80 ANTFRTPYGNFLISQAPNDST-NADHLHLMWIYNVQTVVCLVLPEEAGGYFVPKEG--ET 136
Query: 441 LCTLSNYGIRTISAFYEQ 494
+ Y I+TI F E+
Sbjct: 137 FTSFQKYQIKTIGIFDEK 154
>AF003134-5|AAB54139.1| 357|Caenorhabditis elegans Nek (never in
mitosis kinase) likeprotein 2 protein.
Length = 357
Score = 27.9 bits (59), Expect = 7.4
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = -3
Query: 562 RTISLKCPDVSPFKSDSRLLPYSCS*NADMVRI 464
+T + K PDVS SD +LPY S + D+ RI
Sbjct: 249 KTHTDKRPDVSQLLSDPLVLPYLISIHCDLGRI 281
>U21321-3|AAG00052.2| 655|Caenorhabditis elegans Hypothetical
protein ZK177.4 protein.
Length = 655
Score = 27.5 bits (58), Expect = 9.8
Identities = 11/27 (40%), Positives = 19/27 (70%)
Frame = +3
Query: 273 KTNYGKDLISELKSELTGNLENVIVAL 353
KT++G+D +S+ + TG+LE + AL
Sbjct: 437 KTSFGRDSVSQPPMKSTGSLEQQLAAL 463
Database: celegans
Posted date: Oct 23, 2007 1:18 PM
Number of letters in database: 12,740,198
Number of sequences in database: 27,780
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,352,868
Number of Sequences: 27780
Number of extensions: 352235
Number of successful extensions: 985
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 919
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 979
length of database: 12,740,198
effective HSP length: 79
effective length of database: 10,545,578
effective search space used: 1613473434
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -