Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--1867
(719 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 290 2e-79
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 283 2e-77
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 147 1e-36
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 129 5e-31
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.2
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.2
SPAC977.12 |||L-asparaginase |Schizosaccharomyces pombe|chr 1|||... 25 8.2
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 8.2
SPBPB8B6.05c |||L-asparaginase |Schizosaccharomyces pombe|chr 2|... 25 8.2
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 290 bits (711), Expect = 2e-79
Identities = 127/188 (67%), Positives = 154/188 (81%)
Frame = +2
Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 256
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 257 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 436
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 437 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVFTA 616
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQV T+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 617 VVEPYNSI 640
VVEPYNS+
Sbjct: 181 VVEPYNSV 188
Score = 29.9 bits (64), Expect = 0.38
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = +3
Query: 672 DCAFMVDNEAIYDI 713
DC FMVDNE+ YDI
Sbjct: 199 DCTFMVDNESCYDI 212
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 283 bits (693), Expect = 2e-77
Identities = 128/193 (66%), Positives = 156/193 (80%), Gaps = 5/193 (2%)
Frame = +2
Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 241
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 242 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 421
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 422 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 601
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 602 QVFTAVVEPYNSI 640
QV T+VVEPYNS+
Sbjct: 180 QVSTSVVEPYNSV 192
Score = 31.1 bits (67), Expect = 0.17
Identities = 12/14 (85%), Positives = 12/14 (85%)
Frame = +3
Query: 672 DCAFMVDNEAIYDI 713
DC FMVDNEA YDI
Sbjct: 203 DCTFMVDNEACYDI 216
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 147 bits (357), Expect = 1e-36
Identities = 72/187 (38%), Positives = 103/187 (55%)
Frame = +2
Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 256
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 257 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 436
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 437 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVFTA 616
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 617 VVEPYNS 637
VVEPYN+
Sbjct: 179 VVEPYNA 185
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 129 bits (311), Expect = 5e-31
Identities = 70/190 (36%), Positives = 108/190 (56%), Gaps = 3/190 (1%)
Frame = +2
Query: 80 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 259
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 260 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 433
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 434 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VF 610
D I + AD L+GF + H L+ERL+ Y KK ++++P Q V
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 611 TAVVEPYNSI 640
VV+PYNS+
Sbjct: 180 DVVVQPYNSL 189
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 6.2
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 156 STASSLMARCPQTRPSGVETILSTLSSARPELAST 260
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.2
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 175 WPDAHRQDHRGWRRFFQHFLQR 240
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC977.12 |||L-asparaginase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 356
Score = 25.4 bits (53), Expect = 8.2
Identities = 13/56 (23%), Positives = 24/56 (42%)
Frame = -2
Query: 688 TMKAQSRVLKGCVGGKNRVVGLDDGSENLRRGVDGELQFRLLAVVNGETLHQ*GSE 521
T+ A + GC +V GS + + ++ + +A +NG + GSE
Sbjct: 39 TVFAMGGTIAGCANSSLEIVNYIPGSVGIEKLIEAVPAIKAIANINGVQVTNMGSE 94
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 8.2
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +3
Query: 66 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 188
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPBPB8B6.05c |||L-asparaginase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 356
Score = 25.4 bits (53), Expect = 8.2
Identities = 13/56 (23%), Positives = 24/56 (42%)
Frame = -2
Query: 688 TMKAQSRVLKGCVGGKNRVVGLDDGSENLRRGVDGELQFRLLAVVNGETLHQ*GSE 521
T+ A + GC +V GS + + ++ + +A +NG + GSE
Sbjct: 39 TVFAMGGTIAGCANSSLEIVNYIPGSVGIEKLIEAVPAIKAIANINGVQVTNMGSE 94
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,939,647
Number of Sequences: 5004
Number of extensions: 60292
Number of successful extensions: 174
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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