Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--1850
(635 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 285 4e-78
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 274 7e-75
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 148 6e-37
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 123 2e-29
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 28 1.3
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.2
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 5.2
SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|c... 25 6.9
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 6.9
SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 25 9.1
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 285 bits (699), Expect = 4e-78
Identities = 127/186 (68%), Positives = 150/186 (80%)
Frame = +2
Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 256
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 257 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 436
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 437 RIRKLADQCTGLQGFLIFHSFGGGPALGSLPY*WSVSPLTTAKKSKLEFAIYPAPQVSTA 616
+IR++AD C+GLQGFL+FHSFGGG G + KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 617 VVEPYN 634
VVEPYN
Sbjct: 181 VVEPYN 186
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 274 bits (672), Expect = 7e-75
Identities = 127/191 (66%), Positives = 151/191 (79%), Gaps = 5/191 (2%)
Frame = +2
Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 241
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 242 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 421
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 422 DLVLDRIRKLADQCTGLQGFLIFHSFGGGPALGSLPY*WSVSPLTTAKKSKLEFAIYPAP 601
D VL+RIR++AD C+GLQGFL+FHSFGGG G + KKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 602 QVSTAVVEPYN 634
QVST+VVEPYN
Sbjct: 180 QVSTSVVEPYN 190
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 148 bits (359), Expect = 6e-37
Identities = 76/186 (40%), Positives = 102/186 (54%)
Frame = +2
Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 256
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 257 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 436
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 437 RIRKLADQCTGLQGFLIFHSFGGGPALGSLPY*WSVSPLTTAKKSKLEFAIYPAPQVSTA 616
+R+ A+ C LQGF + HS GGG G S + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 617 VVEPYN 634
VVEPYN
Sbjct: 179 VVEPYN 184
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 123 bits (296), Expect = 2e-29
Identities = 69/188 (36%), Positives = 105/188 (55%), Gaps = 3/188 (1%)
Frame = +2
Query: 80 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 259
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 260 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 433
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 434 DRIRKLADQCTGLQGFLIFHSFGGGPALGSLPY*WSVSPLTTAKKSKLEFAIYPAPQ-VS 610
D I + AD L+GF + HS GG G + KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 611 TAVVEPYN 634
VV+PYN
Sbjct: 180 DVVVQPYN 187
>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 830
Score = 27.9 bits (59), Expect = 1.3
Identities = 13/36 (36%), Positives = 24/36 (66%)
Frame = -2
Query: 535 SIRK*TQSRTSTEGVEDQESLQTGTLVSELADSVQN 428
SIR ++SR+S+ G + Q S+Q+GT ++D ++
Sbjct: 108 SIRSQSRSRSSSVGSDSQASIQSGTNSEVVSDDEED 143
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 5.2
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 156 STASSLMARCPQTRPSGVETILSTLSSARPELAST 260
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 5.2
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 175 WPDAHRQDHRGWRRFFQHFLQR 240
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|chr
2|||Manual
Length = 559
Score = 25.4 bits (53), Expect = 6.9
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = +2
Query: 536 WSVSPLTTAKKSKLEFAIYPAPQVSTAVVEP 628
W ++ + AK++ LE A+YP P+ + +V+P
Sbjct: 515 WGLNLVFPAKETLLEEAVYP-PKSNAELVDP 544
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 6.9
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +3
Query: 66 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 188
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 400
Score = 25.0 bits (52), Expect = 9.1
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -2
Query: 130 ITDLDSGLANVYRDALTHFDLSF 62
+T +D GLA YRD TH + +
Sbjct: 146 VTMIDFGLAKKYRDFKTHVHIPY 168
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,732,114
Number of Sequences: 5004
Number of extensions: 59286
Number of successful extensions: 177
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 167
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 283719918
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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