Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--1818
(726 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 128 1e-30
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 106 4e-24
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 105 5e-24
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 104 1e-23
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 87 2e-18
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 87 2e-18
SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 39 6e-04
SPCC737.06c |||glutamate-cysteine ligase regulatory subunit |Sch... 34 0.018
SPAC977.14c |||aldo/keto reductase, unknown biological role|Schi... 31 0.22
SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 26 6.3
SPBC336.11 |||GARP complex subunit Vps52 |Schizosaccharomyces po... 26 6.3
SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 25 8.3
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 25 8.3
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 128 bits (308), Expect = 1e-30
Identities = 69/169 (40%), Positives = 96/169 (56%)
Frame = +3
Query: 99 VVDYALEAGYRMFDTAAVYGNEIHLGEAFKTLLPKYDLEREDIFVTTKLSPSDHGSRAEH 278
+V AL++GYR DTAAVYGNE G+A K +++R DIF+T+KL+
Sbjct: 37 LVTQALDSGYRHIDTAAVYGNEDICGKAIVDWCEKNNVKRTDIFLTSKLANCSDYYSTRA 96
Query: 279 AYQKSLENLDLEYVDLYLIHFPGGAKLPAEDKRNTKMRELSWCKLTELYDDGKVNAVGVS 458
A + SL +L Y+DL+LI P G K K R SW + E D G + +VGVS
Sbjct: 97 AIRSSLHHLGT-YIDLFLIQSPAGGK---------KSRIASWKAMEEFVDSGDIRSVGVS 146
Query: 459 NFTVAHLEGLMESNHGLVPAVNQVEWHPYYTQSSLYKFCKQHHITLQAY 605
N+ V HL+ L SN P VNQ+E HP+ +Q + K+C+ H I ++AY
Sbjct: 147 NYGVKHLQELYASNPKFYPCVNQIELHPFLSQDDIVKYCQSHDIAIEAY 195
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 106 bits (254), Expect = 4e-24
Identities = 69/208 (33%), Positives = 114/208 (54%), Gaps = 10/208 (4%)
Frame = +3
Query: 48 TLEVGTYRIRQSDIVMQVVDYALEAGYRMFDTAAVYGNEIHLGEAFKTLLPKYDLEREDI 227
++ +GT+R + D V AL+AGYR DTA +YGNE +GE + + + R DI
Sbjct: 25 SIGLGTWRSGK-DETKNAVCAALKAGYRHIDTAHIYGNEKEIGEGIR----ESGVPRTDI 79
Query: 228 FVTTKLSPSDH-GSRAEHAYQKSLENLDLEYVDLYLIHFP-----GGAKLPAEDKRNTKM 389
+VT+KL + H A +K+L++L+LEY+D YLIH+P G +LP +K
Sbjct: 80 WVTSKLWCNAHRAGLVPLALEKTLQDLNLEYIDAYLIHWPFALLSGPEELPRNEKGELIY 139
Query: 390 REL----SWCKLTELYDDGKVNAVGVSNFTVAHLEGLMESNHGLVPAVNQVEWHPYYTQS 557
++ +W + EL + GKV +G+SNF +L+ +++ + P ++Q+E HPY Q+
Sbjct: 140 EDVPIEETWQAMEELLETGKVRYIGISNFNNEYLDRVLKIAK-VKPTIHQMELHPYLPQT 198
Query: 558 SLYKFCKQHHITLQAYCSFGGTSCSNNS 641
+ K+ I + AY + + NS
Sbjct: 199 EYLEKHKKLQIHVSAYSPLANQNDAYNS 226
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 105 bits (253), Expect = 5e-24
Identities = 62/197 (31%), Positives = 101/197 (51%), Gaps = 1/197 (0%)
Frame = +3
Query: 60 GTYRIRQSDIVMQVVDYALEAGYRMFDTAAVYGNEIHLGEAFKTLLPKYDLEREDIFVTT 239
G+Y + ++ V AL+ GYR D+A +Y NE G A + + +REDI+ T+
Sbjct: 20 GSYMVNRTKCFDSVYA-ALQCGYRHIDSAQMYHNEADCGRAILKFMEETGTKREDIWFTS 78
Query: 240 KLSPSDHGSRAEHAYQKSLENLDLEYVDLYLIHFPGGAKLPAEDKRNTKMRELSWCKLTE 419
KL+ + S++ L Y+DL+L+H P G ++ SW L +
Sbjct: 79 KLNDLSGYKSTLSSIDASVKACGLGYIDLFLLHSPYGDRIE------------SWKALEK 126
Query: 420 LYDDGKVNAVGVSNFTVAHLEGLMESNHGLVPAVNQVEWHPYYTQSSLYKFCKQHHITLQ 599
++GK+ A+GVSNF H++ L++S+ ++P VNQ+E HP+ +Q + +C+ I L
Sbjct: 127 GVEEGKLRAIGVSNFGPHHIQELLDSHPKIIPCVNQIELHPFCSQQKVVDYCESKGIQLA 186
Query: 600 AYCSF-GGTSCSNNSLL 647
AY G N LL
Sbjct: 187 AYAPLVHGEKFGNKQLL 203
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 104 bits (249), Expect = 1e-23
Identities = 70/199 (35%), Positives = 109/199 (54%), Gaps = 10/199 (5%)
Frame = +3
Query: 51 LEVGTYRIRQSDIVMQVVDYALEAGYRMFDTAAVYGNEIHLGEAFKTLLPKYDLEREDIF 230
L +GT+R + V AL+ GYR D AA+YGNE +G+ K + + R+DI+
Sbjct: 27 LGLGTWRSEPNQ-TKNAVKTALQYGYRHIDAAAIYGNEDEVGDGIK----ESGVPRKDIW 81
Query: 231 VTTKLSPSDHGSRA-EHAYQKSLENLDLEYVDLYLIHFP-----GGAKLPAEDKRNTKMR 392
VT+KL + H A A +K+L++L L+Y+D YLIH+P G K P + N
Sbjct: 82 VTSKLWCNAHAPEAVPKALEKTLKDLKLDYLDEYLIHWPVSFKTGEDKFPKDKDGNLIYE 141
Query: 393 ----ELSWCKLTELYDDGKVNAVGVSNFTVAHLEGLMESNHGLVPAVNQVEWHPYYTQSS 560
E +W + +L + GKV +G+SNF +LE +++ + PAV+Q+E HP+ Q+
Sbjct: 142 KNPIEETWKAMEKLLETGKVRHIGLSNFNDTNLERILKVAK-VKPAVHQMELHPFLPQTE 200
Query: 561 LYKFCKQHHITLQAYCSFG 617
+ K+ I + AY FG
Sbjct: 201 FVEKHKKLGIHVTAYSPFG 219
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 87.4 bits (207), Expect = 2e-18
Identities = 58/174 (33%), Positives = 93/174 (53%), Gaps = 2/174 (1%)
Frame = +3
Query: 90 VMQVVDYALEAGYRMFDTAAVYGNEIHLGEAFKTLLPKYDLEREDIFVTTKLSPSDHGSR 269
++ V AL AG+ D A VYGNE +G A K + ++ R +F+T+K+ +
Sbjct: 33 IVDSVKNALAAGFIHIDCAEVYGNEEEVGVALK----EANVPRSKLFITSKV--MHNVDN 86
Query: 270 AEHAYQKSLENLDLEYVDLYLIHFPGGAKLPAEDKRNTKMRELSWCKLTELYDDGKVNAV 449
A +SL L +Y+DLYL+H P +P +K+ + E W + G V++V
Sbjct: 87 IPEALNESLRKLGTDYLDLYLLHSP----IPFYEKK-IPISE-GWKAMETALGTGLVHSV 140
Query: 450 GVSNFTVAHLEGLMESNHGLVPAVNQVEWHP--YYTQSSLYKFCKQHHITLQAY 605
GVSNF + LE L++++ + P VNQ+E+HP Y L +FC+ I ++ Y
Sbjct: 141 GVSNFRIPDLEELLKTS-TITPRVNQIEFHPQVYKAAKPLVEFCQSKGIIVEGY 193
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 87.0 bits (206), Expect = 2e-18
Identities = 61/190 (32%), Positives = 98/190 (51%), Gaps = 2/190 (1%)
Frame = +3
Query: 42 KQTLEVGTYRIRQSDIVMQVVDYALEAGYRMFDTAAVYGNEIHLGEAFKTLLPK-YDLER 218
K+ L G Y+++ D QV++ AL G R+ D+A Y NE +A + + +++R
Sbjct: 13 KEQLCFGVYQLK--DCYQQVIE-ALSLGIRVIDSAITYRNEKECEQAIQDFCHQNVNIKR 69
Query: 219 EDIFVTTKLSPSDHG-SRAEHAYQKSLENLDLEYVDLYLIHFPGGAKLPAEDKRNTKMRE 395
EDI + TK+ S G R A ++SL +D+ LIH P K P R
Sbjct: 70 EDITLITKIPDSLQGFERTWKAVEQSLRRTGRPKLDVVLIHSP---KWPVR-------RI 119
Query: 396 LSWCKLTELYDDGKVNAVGVSNFTVAHLEGLMESNHGLVPAVNQVEWHPYYTQSSLYKFC 575
SW L + +G++N +GVSN+ + HLE ++ L PA+NQVE+ + + + +C
Sbjct: 120 ESWRALLQHQKEGRINKIGVSNYNIHHLEEIISLGLPL-PAINQVEFSAFNNRPTFLSYC 178
Query: 576 KQHHITLQAY 605
H I +QA+
Sbjct: 179 FNHGILVQAF 188
>SPCC965.06 |||potassium channel subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 344
Score = 39.1 bits (87), Expect = 6e-04
Identities = 45/169 (26%), Positives = 71/169 (42%), Gaps = 16/169 (9%)
Frame = +3
Query: 111 ALEAGYRMFDTAAVYGN---EIHLGEAFKTLLPKYDLEREDIFVTTKLS-------PSDH 260
A + G FDTA +Y N E +G+A K L +R + +TTK+ P+
Sbjct: 53 AWDLGINTFDTAEIYSNGNSETVMGKAIKEL----GWDRSEYVITTKVFFGAGTKLPNTT 108
Query: 261 GSRAEHAYQ---KSLENLDLEYVDLYLIHFPGGAKLPAEDKRNTKMRELSWCKLTELYDD 431
G +H + SL+ L L YVD+ + H P +P E+ T+L D
Sbjct: 109 GLSRKHIIEGLNASLKRLGLPYVDVIMAHRP-DPSVPMEEVVRA---------FTQLIQD 158
Query: 432 GKVNAVGVSN---FTVAHLEGLMESNHGLVPAVNQVEWHPYYTQSSLYK 569
GK G S F + H + + + P +Q +++ Y T+ K
Sbjct: 159 GKAFYWGTSEWSAFEIEHAHHIATKYNLIAPVADQPQYN-YLTRDHFEK 206
>SPCC737.06c |||glutamate-cysteine ligase regulatory subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 287
Score = 34.3 bits (75), Expect = 0.018
Identities = 21/67 (31%), Positives = 33/67 (49%), Gaps = 1/67 (1%)
Frame = +3
Query: 399 SWCKLTELYDDGKVNAVGVSNFTVAHLEGLMESNHGLVPAVNQVE-WHPYYTQSSLYKFC 575
+W L E +GK+ +GVS F V L+ L+ S+ +VP Q+ + L F
Sbjct: 161 TWKLLEEKVGEGKIGTLGVSEFGVNELQRLI-SSVNVVPESTQINIGQNCKLPNDLLNFA 219
Query: 576 KQHHITL 596
+HH+ L
Sbjct: 220 DRHHLKL 226
>SPAC977.14c |||aldo/keto reductase, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 351
Score = 30.7 bits (66), Expect = 0.22
Identities = 16/57 (28%), Positives = 28/57 (49%)
Frame = +3
Query: 72 IRQSDIVMQVVDYALEAGYRMFDTAAVYGNEIHLGEAFKTLLPKYDLEREDIFVTTK 242
+ + V +++ A +AG R FDTA Y + E + KY++ R I + +K
Sbjct: 40 LEDEEEVFKIMKAAYDAGIRTFDTANCYSAGVS-EELVGKFIRKYEIPRSSIVILSK 95
>SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 503
Score = 25.8 bits (54), Expect = 6.3
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = -2
Query: 485 PFKMCHRKVGYPYGIDFTIIIQLCELTP 402
PF MC K+ TII++ ELTP
Sbjct: 173 PFLMCAWKIAPAVACGNTIILKTAELTP 200
>SPBC336.11 |||GARP complex subunit Vps52 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 508
Score = 25.8 bits (54), Expect = 6.3
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = +2
Query: 584 PHYSSSVLFLW 616
PHY+S +LFLW
Sbjct: 372 PHYNSMILFLW 382
>SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated
membrane proteins, ESCRT 0 complex|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 610
Score = 25.4 bits (53), Expect = 8.3
Identities = 10/26 (38%), Positives = 11/26 (42%)
Frame = +1
Query: 370 TNAIRRCENCRGVSSQSCMMMVKSMP 447
TN C NC GV C S+P
Sbjct: 186 TNRKHHCRNCGGVFCNQCSSKTLSLP 211
>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1727
Score = 25.4 bits (53), Expect = 8.3
Identities = 18/55 (32%), Positives = 31/55 (56%), Gaps = 2/55 (3%)
Frame = +3
Query: 159 NEIHLGEAFKTLLPKYDLEREDIFVTT-KLSPSDHG-SRAEHAYQKSLENLDLEY 317
N+ L +A + + K++ E+E + ++ KL S+ S H+ QKSLE +EY
Sbjct: 797 NQQQLSDAARKV--KFESEKESLSLSLQKLKESNEKMSNDLHSLQKSLEKSGIEY 849
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,096,317
Number of Sequences: 5004
Number of extensions: 65725
Number of successful extensions: 239
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 218
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 226
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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