Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--1768
(494 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 246 1e-66
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 239 1e-64
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 124 7e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 87 1e-18
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 33 0.024
SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosacch... 26 3.6
SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po... 25 4.7
SPCC1281.07c |||glutathione S-transferase Gst3|Schizosaccharomyc... 25 8.3
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 246 bits (602), Expect = 1e-66
Identities = 112/164 (68%), Positives = 134/164 (81%)
Frame = +2
Query: 2 NYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDY 181
NYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH LL+ERL+++Y
Sbjct: 106 NYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEY 165
Query: 182 GKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCALMDDNEAIYDICRRNLDIER 361
GKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC M DNEA YDICRRNLDIER
Sbjct: 166 GKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIER 225
Query: 362 PTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQN*LGALPPV 493
PTY NLNRLI Q+VSSITASLRF G+LNVDL EFQ L P +
Sbjct: 226 PTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRI 269
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 239 bits (586), Expect = 1e-64
Identities = 109/164 (66%), Positives = 133/164 (81%)
Frame = +2
Query: 2 NYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDY 181
NYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH LL+ERL+++Y
Sbjct: 102 NYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEY 161
Query: 182 GKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCALMDDNEAIYDICRRNLDIER 361
KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC M DNE+ YDICRRNLDIER
Sbjct: 162 TKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIER 221
Query: 362 PTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQN*LGALPPV 493
P+Y NLNRLI Q+VSSITASLRF+G+LNVDL EFQ L P +
Sbjct: 222 PSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRI 265
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 124 bits (299), Expect = 7e-30
Identities = 59/150 (39%), Positives = 87/150 (58%)
Frame = +2
Query: 5 YARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYG 184
+A+GHYT G E+ D VLD +R+ A+ C LQGF + H LL+ ++ +Y
Sbjct: 101 WAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYP 160
Query: 185 KKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCALMDDNEAIYDICRRNLDIERP 364
+ F++ PAP+ S VVEPYN+ L+ H +E+SD DNEA+ I L I+ P
Sbjct: 161 DRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSP 220
Query: 365 TYTNLNRLIGQIVSSITASLRFDGALNVDL 454
+Y +LN L+ +++ +T S RF G LN DL
Sbjct: 221 SYDDLNHLVSAVMAGVTTSFRFPGELNSDL 250
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 87.0 bits (206), Expect = 1e-18
Identities = 49/152 (32%), Positives = 81/152 (53%), Gaps = 1/152 (0%)
Frame = +2
Query: 2 NYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDY 181
N+A G Y+ + I + ++D I + AD L+GF + H L+ERL+ Y
Sbjct: 103 NWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRY 161
Query: 182 GKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCALMDDNEAIYDICRRNLDIE 358
KK ++++P Q VS VV+PYNS+L ++D ++ DN A+ I L +
Sbjct: 162 PKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQ 221
Query: 359 RPTYTNLNRLIGQIVSSITASLRFDGALNVDL 454
PT+ N+L+ ++S+ T +LR+ G +N DL
Sbjct: 222 NPTFHQQNQLVSTVMSASTTTLRYPGYMNNDL 253
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 33.1 bits (72), Expect = 0.024
Identities = 18/51 (35%), Positives = 29/51 (56%)
Frame = -2
Query: 472 LVLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 320
L E + H ++ + Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 168 LQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPBC12C2.05c |||diacylglycerol binding protein Bzz1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 25.8 bits (54), Expect = 3.6
Identities = 25/84 (29%), Positives = 39/84 (46%), Gaps = 6/84 (7%)
Frame = -2
Query: 439 SAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADV---IDGFIVVHQSTVR---VLKG 278
+A ES R++ D S Q + E T A+ +DG +V++ T V+
Sbjct: 478 NAYESYRNTYTDDMDSSSIYQTSLSNVKTEETTPAEPASKVDG-VVLYDFTGEHEGVITA 536
Query: 277 CVGGEDRVVGLDDGSGNLRRGVDG 206
G E ++ DDGSG +R +DG
Sbjct: 537 SEGQEFTLLEPDDGSGWVRVKIDG 560
>SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 949
Score = 25.4 bits (53), Expect = 4.7
Identities = 20/69 (28%), Positives = 34/69 (49%)
Frame = +2
Query: 227 VSTAVVEPYNSILTTHTTLEHSDCALMDDNEAIYDICRRNLDIERPTYTNLNRLIGQIVS 406
VS +VV+ ++ LT+ H ++D NE + DI Y + NRLI +
Sbjct: 367 VSQSVVQQFSKFLTSDM---HKFSTILDSNE-LKDI-----------YLSKNRLIPSVTK 411
Query: 407 SITASLRFD 433
S+ +++ FD
Sbjct: 412 SLPSAMEFD 420
>SPCC1281.07c |||glutathione S-transferase Gst3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 313
Score = 24.6 bits (51), Expect = 8.3
Identities = 14/41 (34%), Positives = 20/41 (48%), Gaps = 3/41 (7%)
Frame = -2
Query: 121 EGVEDQESLQTGTLVSELA---DSVQNQIYDFLSNGVVTTG 8
E +ED+E S L D + + YD ++NGV TG
Sbjct: 147 EVIEDEEKRVVDLYPSSLRTKIDELNDYFYDTVNNGVYKTG 187
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,946,078
Number of Sequences: 5004
Number of extensions: 36168
Number of successful extensions: 124
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 121
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 194131776
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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