BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--1400 (744 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g76010.1 68414.m08825 expressed protein 29 2.5 At4g30770.1 68417.m04360 hypothetical protein 28 5.7 At5g45140.1 68418.m05542 DNA-directed RNA polymerase, putative s... 28 7.5 At5g40480.1 68418.m04909 expressed protein ; expression supporte... 28 7.5 At5g09760.1 68418.m01130 pectinesterase family protein contains ... 27 9.9 At1g12220.1 68414.m01414 disease resistance protein RPS5 (CC-NBS... 27 9.9 >At1g76010.1 68414.m08825 expressed protein Length = 350 Score = 29.5 bits (63), Expect = 2.5 Identities = 23/84 (27%), Positives = 42/84 (50%), Gaps = 2/84 (2%) Frame = +3 Query: 258 KVIDPKTRKEMPLDKALELRLIDHVGRYKYITETITMLQAIESKYIVFIHLTQSVSSQKV 437 +V+ PK + P+D A E+R+ YIT +T+LQ S +VF + ++++ Sbjct: 6 RVVKPKA--DTPID-ANEIRITSQGRARNYITYAMTLLQDKGSTEVVFKAMGRAINKTVT 62 Query: 438 ITITSVAGMPDKMEISEI--SDVT 503 I +PD + + I +D+T Sbjct: 63 IVELIKRRIPDLHQNTSIGSTDIT 86 >At4g30770.1 68417.m04360 hypothetical protein Length = 157 Score = 28.3 bits (60), Expect = 5.7 Identities = 17/62 (27%), Positives = 27/62 (43%) Frame = +2 Query: 476 GNFGNIRRDHVPRDLEIEEQVALEPVQVAPGVIFDPATALVISTPSGVSENIIEAAYNGT 655 GN N + + RDL+ EQ P P I P +++S S+ + + N T Sbjct: 82 GNNNNANGETMTRDLDPPEQSLESPDDDDPEPIAHPQGQVLVSIEGNQSKKRMSTSSNST 141 Query: 656 VA 661 +A Sbjct: 142 IA 143 >At5g45140.1 68418.m05542 DNA-directed RNA polymerase, putative similar to SP|P22276 DNA-directed RNA polymerase III 130 kDa polypeptide (EC 2.7.7.6) (RNA polymerase III subunit 2) {Saccharomyces cerevisiae}; contains Pfam profiles PF04560: RNA polymerase Rpb2 domain 7, PF04561: RNA polymerase Rpb2 domain 2, PF04565: RNA polymerase Rpb2 domain 3, PF04566: RNA polymerase Rpb2 domain 4, PF04567: RNA polymerase Rpb2 domain 5 Length = 1150 Score = 27.9 bits (59), Expect = 7.5 Identities = 13/29 (44%), Positives = 19/29 (65%) Frame = +1 Query: 175 GMFVIPGTDRVLDLEEALKLNLINPESQK 261 G F+I GT++VL ++E L N I +S K Sbjct: 176 GYFIIKGTEKVLLIQEQLSKNRIIIDSDK 204 >At5g40480.1 68418.m04909 expressed protein ; expression supported by MPSS Length = 1919 Score = 27.9 bits (59), Expect = 7.5 Identities = 19/84 (22%), Positives = 37/84 (44%), Gaps = 4/84 (4%) Frame = +1 Query: 1 VSQPRESVVQEIRTVTSKSVVTEPSVTSMTIKKNTIELPRGGWTLNEAIHLNFFDPTT-- 174 V + SV + + T + K +T++ + GG L++ I + + P Sbjct: 1092 VDEDSLSVGEHVATSSFKIAARRLGITTLYVSARQ---QSGGKVLSQTIKVEVYSPPRLH 1148 Query: 175 --GMFVIPGTDRVLDLEEALKLNL 240 G+F++PG VL +E +N+ Sbjct: 1149 PQGIFLVPGASYVLTIEGGPTMNV 1172 >At5g09760.1 68418.m01130 pectinesterase family protein contains Pfam profile: PF01095 pectinesterase Length = 551 Score = 27.5 bits (58), Expect = 9.9 Identities = 10/26 (38%), Positives = 17/26 (65%) Frame = +2 Query: 554 QVAPGVIFDPATALVISTPSGVSENI 631 + PG+ DP+T L + PSG+ E++ Sbjct: 216 KTGPGLGLDPSTGLNLGFPSGLKEDV 241 >At1g12220.1 68414.m01414 disease resistance protein RPS5 (CC-NBS-LRR class) / resistance to Pseudomonas syringae protein 5 (CC-NBS-LRR class) domain signature CC-NBS-LRR exists, suggestive of a disease resistance protein. Identical to RPS5 (resistance to Pseudomonas syringae protein 5)(gi:3309620) Length = 889 Score = 27.5 bits (58), Expect = 9.9 Identities = 29/103 (28%), Positives = 51/103 (49%), Gaps = 5/103 (4%) Frame = +1 Query: 109 ELPRGGWTLNEAIHLNFFDPTTGMFVIPGTDRVLDLEEALKLNLINPESQKSLTPKLVKK 288 +LP G WTL + IHLN + + + I G + +L L L +S+ L LVK+ Sbjct: 601 QLPVGLWTLKKLIHLN-LEHMSSLGSILGISNLWNL-RTLGLR----DSRLLLDMSLVKE 654 Query: 289 CH*IKHSNYV*L-----IMSVVINISLKLLQCYKQ*KVNILYL 402 ++H + L +++ + S +L++C K+ V+ YL Sbjct: 655 LQLLEHLEVITLDISSSLVAEPLLCSQRLVECIKE--VDFKYL 695 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,643,368 Number of Sequences: 28952 Number of extensions: 263156 Number of successful extensions: 709 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 696 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 709 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1643603136 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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