Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--1301
(625 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P... 126 1e-29
At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P... 126 1e-29
At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id... 126 2e-29
At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id... 126 2e-29
At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 47 1e-05
At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative... 38 0.005
At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (... 38 0.005
At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1) 30 1.4
At5g23575.1 68418.m02766 transmembrane protein, putative similar... 28 5.8
At5g08500.1 68418.m01007 transmembrane CLPTM1 family protein con... 28 5.8
>At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 126 bits (304), Expect = 1e-29
Identities = 67/123 (54%), Positives = 75/123 (60%)
Frame = +3
Query: 255 ILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGR 434
ILGADTRATE +V+DKNC+KIHY+A N+YCCGAGTAADTE T V+SQL L R TGR
Sbjct: 52 ILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGR 111
Query: 435 TVPAETXATLLKRMLFRYQGHIGAAWFWEVFDRTGLIFTAFILMDL*INYHMQPWGSGSL 614
T TLLK+ LF YQGH+ AA D TG GSGSL
Sbjct: 112 DSRVVTALTLLKKHLFSYQGHVSAALVLGGVDITGPHLHTIYPHGSTDTLPFATMGSGSL 171
Query: 615 AAM 623
AAM
Sbjct: 172 AAM 174
Score = 54.4 bits (125), Expect = 6e-08
Identities = 27/51 (52%), Positives = 33/51 (64%)
Frame = +1
Query: 103 MASALVPEIPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGV 255
M+ + V P GFSF+ +RN L QKG AP KTGTTIVG+I+ DGV
Sbjct: 1 MSQSSVDIPPKGGFSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGV 51
>At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 126 bits (304), Expect = 1e-29
Identities = 67/123 (54%), Positives = 75/123 (60%)
Frame = +3
Query: 255 ILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGR 434
ILGADTRATE +V+DKNC+KIHY+A N+YCCGAGTAADTE T V+SQL L R TGR
Sbjct: 52 ILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGR 111
Query: 435 TVPAETXATLLKRMLFRYQGHIGAAWFWEVFDRTGLIFTAFILMDL*INYHMQPWGSGSL 614
T TLLK+ LF YQGH+ AA D TG GSGSL
Sbjct: 112 DSRVVTALTLLKKHLFSYQGHVSAALVLGGVDITGPHLHTIYPHGSTDTLPFATMGSGSL 171
Query: 615 AAM 623
AAM
Sbjct: 172 AAM 174
Score = 54.4 bits (125), Expect = 6e-08
Identities = 27/51 (52%), Positives = 33/51 (64%)
Frame = +1
Query: 103 MASALVPEIPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGV 255
M+ + V P GFSF+ +RN L QKG AP KTGTTIVG+I+ DGV
Sbjct: 1 MSQSSVDIPPKGGFSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGV 51
>At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 273
Score = 126 bits (303), Expect = 2e-29
Identities = 67/123 (54%), Positives = 75/123 (60%)
Frame = +3
Query: 255 ILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGR 434
ILGADTRATE +V+DKNC+KIHY+A N+YCCGAGTAADTE T V+SQL L R TGR
Sbjct: 52 ILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGR 111
Query: 435 TVPAETXATLLKRMLFRYQGHIGAAWFWEVFDRTGLIFTAFILMDL*INYHMQPWGSGSL 614
T TLLK+ LF YQGH+ AA D TG GSGSL
Sbjct: 112 DSRVITALTLLKKHLFSYQGHVSAALVLGGVDITGPHLHTIYPHGSTDTLPFATMGSGSL 171
Query: 615 AAM 623
AAM
Sbjct: 172 AAM 174
Score = 55.2 bits (127), Expect = 3e-08
Identities = 27/51 (52%), Positives = 33/51 (64%)
Frame = +1
Query: 103 MASALVPEIPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGV 255
M+ + V P GFSF+ +RN L QKG AP KTGTTIVG+I+ DGV
Sbjct: 1 MSQSTVDVPPKGGFSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGV 51
>At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 267
Score = 126 bits (303), Expect = 2e-29
Identities = 67/123 (54%), Positives = 75/123 (60%)
Frame = +3
Query: 255 ILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGR 434
ILGADTRATE +V+DKNC+KIHY+A N+YCCGAGTAADTE T V+SQL L R TGR
Sbjct: 52 ILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGR 111
Query: 435 TVPAETXATLLKRMLFRYQGHIGAAWFWEVFDRTGLIFTAFILMDL*INYHMQPWGSGSL 614
T TLLK+ LF YQGH+ AA D TG GSGSL
Sbjct: 112 DSRVITALTLLKKHLFSYQGHVSAALVLGGVDITGPHLHTIYPHGSTDTLPFATMGSGSL 171
Query: 615 AAM 623
AAM
Sbjct: 172 AAM 174
Score = 55.2 bits (127), Expect = 3e-08
Identities = 27/51 (52%), Positives = 33/51 (64%)
Frame = +1
Query: 103 MASALVPEIPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGV 255
M+ + V P GFSF+ +RN L QKG AP KTGTTIVG+I+ DGV
Sbjct: 1 MSQSTVDVPPKGGFSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGV 51
>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
(PRCD) identical to cDNA proteasome subunit prcd
GI:2511593
Length = 233
Score = 46.8 bits (106), Expect = 1e-05
Identities = 21/76 (27%), Positives = 41/76 (53%)
Frame = +3
Query: 255 ILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGR 434
+LGAD+R + V+++ KI L N+Y C +G+AAD+++ + V L + G+
Sbjct: 25 VLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQHGQ 84
Query: 435 TVPAETXATLLKRMLF 482
+ A L++ + +
Sbjct: 85 PATVKVSANLIRMLAY 100
>At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative
very strong similarity to SP|O23717 Proteasome subunit
beta type 5 precursor (EC 3.4.25.1) (20S proteasome
subunit E) (Proteasome epsilon chain) {Arabidopsis
thaliana}
Length = 273
Score = 37.9 bits (84), Expect = 0.005
Identities = 22/80 (27%), Positives = 40/80 (50%)
Frame = +3
Query: 255 ILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGR 434
++ AD+RA+ +S ++ +KI + M AG AAD + +++ + L L R
Sbjct: 70 MVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKR 129
Query: 435 TVPAETXATLLKRMLFRYQG 494
+ + LL ML+ Y+G
Sbjct: 130 RISVSGASKLLANMLYSYRG 149
>At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1)
(PRCE) identical to GB:O23717; identical to cDNA
proteasome subunit prce GI:2511595
Length = 274
Score = 37.9 bits (84), Expect = 0.005
Identities = 22/80 (27%), Positives = 40/80 (50%)
Frame = +3
Query: 255 ILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGR 434
++ AD+RA+ +S ++ +KI + M AG AAD + +++ + L L R
Sbjct: 70 MVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKR 129
Query: 435 TVPAETXATLLKRMLFRYQG 494
+ + LL ML+ Y+G
Sbjct: 130 RISVSGASKLLANMLYSYRG 149
>At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1)
Length = 223
Score = 29.9 bits (64), Expect = 1.4
Identities = 17/76 (22%), Positives = 33/76 (43%)
Frame = +3
Query: 255 ILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGR 434
++ ADTR + + ++ KIH LA +G AD + + + S+ + + +
Sbjct: 28 VIAADTRMSTGYSILSRDYSKIHKLADRAVLSSSGFQADVKALQKVLKSRHLIYQHQHNK 87
Query: 435 TVPAETXATLLKRMLF 482
+ A LL L+
Sbjct: 88 QMSCPAMAQLLSNTLY 103
>At5g23575.1 68418.m02766 transmembrane protein, putative similar to
cleft lip and palate transmembrane protein 1 [Homo
sapiens] GI:4039014; contains Pfam profile PF05602:
Cleft lip and palate transmembrane protein 1 (CLPTM1)
Length = 593
Score = 27.9 bits (59), Expect = 5.8
Identities = 15/31 (48%), Positives = 21/31 (67%), Gaps = 5/31 (16%)
Frame = -2
Query: 396 QQIVLSFQCQL---LFLLHNNTCWML--SSG 319
+ +VL+F CQ L+LL N+T WM+ SSG
Sbjct: 368 KSVVLNFICQFVIFLYLLDNDTSWMILASSG 398
>At5g08500.1 68418.m01007 transmembrane CLPTM1 family protein
contains Pfam profile PF05602: Cleft lip and palate
transmembrane protein 1 (CLPTM1)
Length = 590
Score = 27.9 bits (59), Expect = 5.8
Identities = 15/31 (48%), Positives = 21/31 (67%), Gaps = 5/31 (16%)
Frame = -2
Query: 396 QQIVLSFQCQL---LFLLHNNTCWML--SSG 319
+ +VL+F CQ L+LL N+T WM+ SSG
Sbjct: 366 KSVVLNFICQFIIFLYLLDNDTSWMILASSG 396
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,769,593
Number of Sequences: 28952
Number of extensions: 284866
Number of successful extensions: 700
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 671
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 696
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1265787216
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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