Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--1223
(557 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 176 2e-45
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 98 7e-22
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 93 2e-20
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 87 2e-18
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 62 7e-11
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 57 2e-09
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 48 9e-07
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 31 0.11
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 30 0.20
SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 29 0.61
SPCC1442.07c |||ubiquitin/metalloprotease fusion protein|Schizos... 28 1.1
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 2.5
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 3.3
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 4.3
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 25 5.7
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 25 10.0
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 25 10.0
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 176 bits (429), Expect = 2e-45
Identities = 78/84 (92%), Positives = 80/84 (95%)
Frame = +3
Query: 252 GQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 431
GQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTE
Sbjct: 48 GQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTE 107
Query: 432 APLNPKANREKMTHIMFETFNTPA 503
APLNPK+NREKMT I+FETFN PA
Sbjct: 108 APLNPKSNREKMTQIIFETFNAPA 131
Score = 99.5 bits (237), Expect = 3e-22
Identities = 42/49 (85%), Positives = 48/49 (97%)
Frame = +1
Query: 115 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGRR 261
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMG++
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQK 50
Score = 31.1 bits (67), Expect = 0.11
Identities = 15/16 (93%), Positives = 15/16 (93%)
Frame = +2
Query: 509 VAIQAVLSLYAFGRTT 556
VAIQAVLSLYA GRTT
Sbjct: 134 VAIQAVLSLYASGRTT 149
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 98.3 bits (234), Expect = 7e-22
Identities = 43/85 (50%), Positives = 59/85 (69%), Gaps = 1/85 (1%)
Frame = +3
Query: 255 QKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTE 431
QKD +VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L PEEHP+LLTE
Sbjct: 53 QKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTE 112
Query: 432 APLNPKANREKMTHIMFETFNTPAM 506
PL N+EK+ +ET N PA+
Sbjct: 113 PPLANIRNKEKIAEYFYETLNVPAL 137
Score = 55.2 bits (127), Expect = 6e-09
Identities = 22/37 (59%), Positives = 29/37 (78%)
Frame = +1
Query: 133 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM 243
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVM 48
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 93.5 bits (222), Expect = 2e-20
Identities = 38/85 (44%), Positives = 60/85 (70%)
Frame = +3
Query: 237 RDGRYGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP 416
+ G KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P
Sbjct: 43 KTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRK 102
Query: 417 VLLTEAPLNPKANREKMTHIMFETF 491
+LLTE P+NP ANREKM MFE +
Sbjct: 103 ILLTEPPMNPVANREKMCETMFERY 127
Score = 48.0 bits (109), Expect = 9e-07
Identities = 21/35 (60%), Positives = 26/35 (74%)
Frame = +1
Query: 121 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 225
E A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
Score = 24.6 bits (51), Expect = 10.0
Identities = 12/13 (92%), Positives = 12/13 (92%)
Frame = +2
Query: 509 VAIQAVLSLYAFG 547
VAIQAVLSLYA G
Sbjct: 134 VAIQAVLSLYAQG 146
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 87.0 bits (206), Expect = 2e-18
Identities = 38/79 (48%), Positives = 49/79 (62%), Gaps = 1/79 (1%)
Frame = +3
Query: 261 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 437
D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE P
Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132
Query: 438 LNPKANREKMTHIMFETFN 494
LNP NRE IMFE+FN
Sbjct: 133 LNPPENRENTAEIMFESFN 151
Score = 40.7 bits (91), Expect = 1e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +1
Query: 133 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 216
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 61.7 bits (143), Expect = 7e-11
Identities = 29/91 (31%), Positives = 53/91 (58%), Gaps = 1/91 (1%)
Frame = +3
Query: 237 RDGRYGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 413
RD + + + VG++ + G ++ + P E +++NWD ME++ +TF +L + EH
Sbjct: 58 RDRKLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEH 116
Query: 414 PVLLTEAPLNPKANREKMTHIMFETFNTPAM 506
P+ +TE NP R MT ++FE +N P++
Sbjct: 117 PICMTEPLANPTYVRSTMTELLFELYNAPSV 147
Score = 35.9 bits (79), Expect = 0.004
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +1
Query: 133 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 228
LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 57.2 bits (132), Expect = 2e-09
Identities = 26/67 (38%), Positives = 36/67 (53%)
Frame = +3
Query: 303 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTHIMF 482
+ +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 483 ETFNTPA 503
E+ PA
Sbjct: 123 ESLRCPA 129
Score = 40.3 bits (90), Expect = 2e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +1
Query: 118 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 219
+EV+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 48.0 bits (109), Expect = 9e-07
Identities = 24/84 (28%), Positives = 44/84 (52%), Gaps = 2/84 (2%)
Frame = +3
Query: 258 KDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 431
++ YV DE Q I + +K +GI+ +W+ W +L+V P E+ +++TE
Sbjct: 49 RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108
Query: 432 APLNPKANREKMTHIMFETFNTPA 503
NP++ R+++ FE + PA
Sbjct: 109 PSWNPQSVRQQIMEAAFEQLHVPA 132
Score = 36.7 bits (81), Expect = 0.002
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +1
Query: 118 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 219
EE+ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 31.1 bits (67), Expect = 0.11
Identities = 15/43 (34%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
Frame = +3
Query: 378 FYNELRVAPEEHPVLLTE--APLNPKANREKMTHIMFETFNTP 500
+Y L E+HP+LLT+ A L P+ + ++ I ++ NTP
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 30.3 bits (65), Expect = 0.20
Identities = 19/65 (29%), Positives = 28/65 (43%), Gaps = 4/65 (6%)
Frame = +3
Query: 318 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTHIMFE 485
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE T FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 486 TFNTP 500
P
Sbjct: 172 ECQVP 176
>SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 277
Score = 28.7 bits (61), Expect = 0.61
Identities = 19/66 (28%), Positives = 29/66 (43%)
Frame = -1
Query: 350 IPVSDDSVFDGVFEGQDTSFALCLISYIRVLLPIPTITP*WRGLPTIEGNTARGASSPAK 171
+P + +V +TSFAL S L PT + P +E + +S+PA
Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122
Query: 170 PALHIP 153
P L+ P
Sbjct: 123 PQLNSP 128
>SPCC1442.07c |||ubiquitin/metalloprotease fusion
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 282
Score = 27.9 bits (59), Expect = 1.1
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -3
Query: 288 SVPHLLHKSPSAHTDHHALMAGPSHDRGEH 199
+VP L P+ HT H + G +H++G H
Sbjct: 148 TVPLLSEMDPAEHTRHDSKTLGLNHNQGAH 177
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 26.6 bits (56), Expect = 2.5
Identities = 15/45 (33%), Positives = 18/45 (40%)
Frame = -1
Query: 248 PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 114
PT+ P P N S P PL+TTN TS+S
Sbjct: 411 PTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 3.3
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = -1
Query: 254 PIPTITP*WRGLPTIEGNTARGASSPAKPAL 162
P+PT P LPT NT + P+ PAL
Sbjct: 74 PVPTGAP---SLPTSSNNTQQAEERPSMPAL 101
>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1828
Score = 25.8 bits (54), Expect = 4.3
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = -2
Query: 160 TYRSHCLLPTRQLLRRTS*ISEI 92
TYR H +PT LL T +SEI
Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 25.4 bits (53), Expect = 5.7
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = +3
Query: 420 LLTEAPLNPKANREKMTHIMFETFN 494
+L APL+ + +KM ++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 24.6 bits (51), Expect = 10.0
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +1
Query: 133 LVVDNGSGMCKAGFAG 180
+V+DNG+ KAGFAG
Sbjct: 8 IVLDNGAYHIKAGFAG 23
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 24.6 bits (51), Expect = 10.0
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = +1
Query: 178 GDDAPRAVFPSIVGRPRHQGVMVGM 252
G+ PRA F ++ P H G+++ M
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM 526
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,529,938
Number of Sequences: 5004
Number of extensions: 55604
Number of successful extensions: 152
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 233995432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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