Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--1222
(578 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 70 1e-12
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 66 2e-11
At4g32280.1 68417.m04592 auxin-responsive AUX/IAA family protein... 28 5.2
At5g10530.1 68418.m01219 lectin protein kinase, putative similar... 27 6.8
At4g25000.1 68417.m03587 alpha-amylase, putative / 1,4-alpha-D-g... 27 6.8
At3g06670.1 68416.m00786 expressed protein 27 6.8
At1g12930.1 68414.m01501 importin-related similar to late gestat... 27 6.8
At5g47770.1 68418.m05901 farnesyl pyrophosphate synthetase 1, mi... 27 9.0
At2g23700.1 68415.m02830 expressed protein contains Pfam profile... 27 9.0
At2g21655.1 68415.m02576 expressed protein contains Pfam PF05617... 27 9.0
>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 69.7 bits (163), Expect = 1e-12
Identities = 38/82 (46%), Positives = 49/82 (59%)
Frame = +1
Query: 4 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 183
MS K EKP L G RIKTRKR+ DP F DA+VQ GDL+ K ++S S L
Sbjct: 1 MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDAVVQIYHDNAGDLELVAKSIES--SDL 57
Query: 184 DYRRYGEVIFDVLIAGGLLLPG 249
++ RYG++ F+V+ GG PG
Sbjct: 58 NFTRYGDIFFEVIFIGGRTQPG 79
>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|P47823
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Oryctolagus
cuniculus}; contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 65.7 bits (153), Expect = 2e-11
Identities = 37/77 (48%), Positives = 47/77 (61%)
Frame = +1
Query: 4 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL 183
MS K EKP L G RIKTRKR+ DP F DALVQ GDL+ + L+S S L
Sbjct: 1 MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDALVQIYLDNAGDLELVARSLES--SDL 57
Query: 184 DYRRYGEVIFDVLIAGG 234
++ RYG++ F+V+ GG
Sbjct: 58 NFSRYGDIFFEVVFIGG 74
>At4g32280.1 68417.m04592 auxin-responsive AUX/IAA family protein
contains Pfam profile: PF02309: AUX/IAA family
Length = 251
Score = 27.9 bits (59), Expect = 5.2
Identities = 13/38 (34%), Positives = 18/38 (47%)
Frame = -3
Query: 339 VRNFAWCPYPHWRRRCRCWSWAIRRPSTRTARQQQAAS 226
+RN CPY H RR + I P+T T ++S
Sbjct: 116 IRNHHHCPYHHRGRRITAMNNNISNPTTATVGSSSSSS 153
>At5g10530.1 68418.m01219 lectin protein kinase, putative similar to
receptor lectin kinase 3 [Arabidopsis thaliana]
gi|4100060|gb|AAD00733; contains protein kinase domain,
Pfam:PF00069; contains legume lectins alpha and beta
domains, Pfam:PF00138 and Pfam:PF00139
Length = 651
Score = 27.5 bits (58), Expect = 6.8
Identities = 18/62 (29%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
Frame = +1
Query: 55 RKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSAGSKL-DYRRYGEVIFDVLIAG 231
+++ +K+K + ++ + LER G YK L SA + D R+ GE F + G
Sbjct: 293 KRKQQKKKAEETENLTSINEDLERGAGPRKFTYKDLASAANNFADDRKLGEGGFGAVYRG 352
Query: 232 GL 237
L
Sbjct: 353 YL 354
>At4g25000.1 68417.m03587 alpha-amylase, putative /
1,4-alpha-D-glucan glucanohydrolase, putative similar to
alpha-amylase from Vigna mungo SP|P17859, Ipomoea nil
GI:21670851; contains Pfam profile PF00128: Alpha
amylase, catalytic domain
Length = 423
Score = 27.5 bits (58), Expect = 6.8
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +1
Query: 64 DEKEKYDPNGFRDALVQGLERAGGDLDAAYKY 159
D K YD N R L Q +E AGG + A+ +
Sbjct: 238 DGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDF 269
>At3g06670.1 68416.m00786 expressed protein
Length = 865
Score = 27.5 bits (58), Expect = 6.8
Identities = 14/57 (24%), Positives = 28/57 (49%), Gaps = 5/57 (8%)
Frame = +3
Query: 294 IFSANEDMDTMRNFEQVF-----VKLMRRYKYLEKMFEEEMKKVLVYLKGFDPEQPH 449
+F ED++ + +F + L+ + LEK+F +E+ ++ +DP PH
Sbjct: 177 VFKICEDLENVDGLHMIFNIVKGIILLNSSQILEKIFGDELIMEIIGCLEYDPGVPH 233
>At1g12930.1 68414.m01501 importin-related similar to late gestation
lung 2 protein (GI:7274209) {Rattus norvegicus}; similar
to Ran binding protein 13 (importin 13)) (GI:8133102)
{Homo sapiens}; contains weak hit to Pfam PF03810:
Importin-beta N-terminal domain
Length = 1005
Score = 27.5 bits (58), Expect = 6.8
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = +3
Query: 528 NIC*RTTWRWTLCWK 572
++C RT+W+ LCWK
Sbjct: 904 SLCERTSWKGMLCWK 918
>At5g47770.1 68418.m05901 farnesyl pyrophosphate synthetase 1,
mitochondrial (FPS1) / FPP synthetase 1 / farnesyl
diphosphate synthase 1 identical to SP|Q09152 Farnesyl
pyrophosphate synthetase 1, mitochondrial precursor (FPP
synthetase 1) (FPS 1) (Farnesyl diphosphate synthetase
1) [Includes: Dimethylallyltransferase (EC 2.5.1.1);
Geranyltranstransferase (EC 2.5.1.10)] {Arabidopsis
thaliana}
Length = 384
Score = 27.1 bits (57), Expect = 9.0
Identities = 12/42 (28%), Positives = 20/42 (47%)
Frame = +2
Query: 233 ACCCRAVRVDGRRIAQDQHLHLLRQ*GYGHHAKFRTGIRETD 358
+CCCR + ++ HLHL G + + ++ ETD
Sbjct: 4 SCCCRNLGKTIKKAIPSHHLHLRSLGGSLYRRRIQSSSMETD 45
>At2g23700.1 68415.m02830 expressed protein contains Pfam profile
PF04784: Protein of unknown function, DUF547
Length = 707
Score = 27.1 bits (57), Expect = 9.0
Identities = 25/85 (29%), Positives = 37/85 (43%), Gaps = 3/85 (3%)
Frame = -3
Query: 567 STKSSARLSFSRCSFTRTKHTDGGTHPLPIHSAVMRGQLDAAVRDRSLSSRPEPFSFPLR 388
ST RL FSR +R D + + + R SL+++P FSF R
Sbjct: 165 STLRGKRLDFSRTPESRCFSFDNRLKSPRLVEKELESP-NLRCRQESLATQPRCFSFDNR 223
Query: 387 TFFPSTC---TDASVSRIPVRNFAW 322
PS+ + VSRI R+F++
Sbjct: 224 LKEPSSAGRQCNQEVSRIDSRSFSF 248
>At2g21655.1 68415.m02576 expressed protein contains Pfam PF05617:
Arabidopsis thaliana protein of unknown function
(DUF784)
Length = 156
Score = 27.1 bits (57), Expect = 9.0
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = -2
Query: 394 SSNIFSKYLYRRISFTNTCSKFRMVSISSLAEKMQVLVLGDSPSID 257
+ N+ SKY ++I++ CSK V + E M ++ S S D
Sbjct: 55 NENVGSKYSKKQINYLMNCSKKMAVPDKCIEEVMAEIIQNKSASRD 100
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,358,491
Number of Sequences: 28952
Number of extensions: 338463
Number of successful extensions: 1317
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1217
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1313
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1131744440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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