Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--1218
(316 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g75850.1 68414.m08810 vacuolar protein sorting-associated pro... 64 2e-11
At2g17790.1 68415.m02062 vacuolar protein sorting-associated pro... 61 2e-10
At3g51310.1 68416.m05616 vacuolar protein sorting-associated pro... 51 1e-07
At5g43630.1 68418.m05333 zinc knuckle (CCHC-type) family protein... 28 1.5
At4g39230.1 68417.m05553 isoflavone reductase, putative similar ... 28 1.5
At4g22217.1 68417.m03213 hypothetical protein 26 4.6
At4g16835.1 68417.m02541 pentatricopeptide (PPR) repeat-containi... 26 4.6
At5g17670.1 68418.m02071 expressed protein 26 6.1
At3g61240.2 68416.m06854 DEAD/DEAH box helicase, putative (RH12)... 26 6.1
At3g61240.1 68416.m06853 DEAD/DEAH box helicase, putative (RH12)... 26 6.1
At3g60740.1 68416.m06795 tubulin folding cofactor D identical to... 25 8.1
At1g68500.1 68414.m07825 expressed protein 25 8.1
>At1g75850.1 68414.m08810 vacuolar protein sorting-associated
protein 35 family protein / VPS35 family protein similar
to vacuolar protein sorting 35 [Mus musculus]
GI:11875394; contains Pfam profile PF03635: Vacuolar
protein sorting-associated protein 35
Length = 838
Score = 63.7 bits (148), Expect = 2e-11
Identities = 32/74 (43%), Positives = 44/74 (59%)
Frame = +1
Query: 1 TFEQINCFGAENAEPMRTQCALAASKLLKKPDQSRAVALCSHLFWKGTRDGRPVWALNYA 180
T ++IN FG EN + + + +++LLKKPDQ RAV CSHLFW DG +
Sbjct: 675 TLQRINVFGVENRDTLTHKATGYSARLLKKPDQCRAVYACSHLFWVDDPDG-----IKDG 729
Query: 181 SRALDCLKKAARVA 222
R L CL++A R+A
Sbjct: 730 ERVLLCLRRALRIA 743
>At2g17790.1 68415.m02062 vacuolar protein sorting-associated
protein 35 family protein / VPS35 family protein similar
to vacuolar protein sorting 35 [Mus musculus]
GI:11875394; contains Pfam profile PF03635: Vacuolar
protein sorting-associated protein 35
Length = 830
Score = 60.9 bits (141), Expect = 2e-10
Identities = 31/74 (41%), Positives = 43/74 (58%)
Frame = +1
Query: 1 TFEQINCFGAENAEPMRTQCALAASKLLKKPDQSRAVALCSHLFWKGTRDGRPVWALNYA 180
T +++ FG EN + + + A+KLLKKPDQ RAV CSHLFW R+ +
Sbjct: 670 TLQRMQVFGVENRDTLTHKATGYAAKLLKKPDQCRAVYACSHLFWLEDRE-----TIQDG 724
Query: 181 SRALDCLKKAARVA 222
R L CLK+A ++A
Sbjct: 725 ERVLLCLKRALKIA 738
>At3g51310.1 68416.m05616 vacuolar protein sorting-associated
protein 35 family protein / VPS35 family protein similar
to vacuolar protein sorting 35 [Mus musculus]
GI:11875394; contains Pfam profile PF03635: Vacuolar
protein sorting-associated protein 35
Length = 783
Score = 51.2 bits (117), Expect = 1e-07
Identities = 26/74 (35%), Positives = 41/74 (55%)
Frame = +1
Query: 1 TFEQINCFGAENAEPMRTQCALAASKLLKKPDQSRAVALCSHLFWKGTRDGRPVWALNYA 180
T +++ F EN + + + +++LL+KPDQ RAV C+HLFW + L
Sbjct: 617 TLQRMRVFNVENRDTLTHKATGYSARLLRKPDQCRAVYECAHLFWADECEN-----LKDG 671
Query: 181 SRALDCLKKAARVA 222
R + CLK+A R+A
Sbjct: 672 ERVVLCLKRAQRIA 685
>At5g43630.1 68418.m05333 zinc knuckle (CCHC-type) family protein
contains Pfam domain PF00098: Zinc knuckle
Length = 831
Score = 27.9 bits (59), Expect = 1.5
Identities = 17/63 (26%), Positives = 28/63 (44%), Gaps = 1/63 (1%)
Frame = +1
Query: 4 FEQINCFGAENAEPMRTQCALAASKLLKKPDQSRAVALCSHLFWKGT-RDGRPVWALNYA 180
FE+ FG++ + + +C + SKL + ++ + WKG D P AL
Sbjct: 272 FEEQLIFGSKRLKTLNQECLGSTSKLKQDSSFMNWISNMTKGIWKGNEEDNSPFVALTTT 331
Query: 181 SRA 189
S A
Sbjct: 332 SNA 334
>At4g39230.1 68417.m05553 isoflavone reductase, putative similar to
allergenic isoflavone reductase-like protein Bet v
6.0102 [Betula pendula][GI:10764491]; contains Pfam
profile PF02716: Isoflavone reductase
Length = 308
Score = 27.9 bits (59), Expect = 1.5
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = -2
Query: 255 VRLHTAVHALLGHAGRLLQAVQRAGRV 175
V + T H+LLGH +++ A++ AG V
Sbjct: 79 VVISTVGHSLLGHQYKIISAIKEAGNV 105
>At4g22217.1 68417.m03213 hypothetical protein
Length = 87
Score = 26.2 bits (55), Expect = 4.6
Identities = 11/32 (34%), Positives = 13/32 (40%)
Frame = +1
Query: 196 CLKKAARVAQQCMDGGVQAHCWPSCWADTRCC 291
C+K R Q DG + PS W CC
Sbjct: 48 CIKFKTRAGQTFFDGKCRPRDRPSVWTACFCC 79
>At4g16835.1 68417.m02541 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 575
Score = 26.2 bits (55), Expect = 4.6
Identities = 17/46 (36%), Positives = 26/46 (56%)
Frame = -2
Query: 246 HTAVHALLGHAGRLLQAVQRAGRVVQRPHRAAVPGPLPEEMRAERH 109
+T + LLG AG+L +A++ + RPH AAV G L R ++
Sbjct: 338 YTCMVDLLGRAGKLEEALKLIRSMPFRPH-AAVFGTLLGACRVHKN 382
>At5g17670.1 68418.m02071 expressed protein
Length = 309
Score = 25.8 bits (54), Expect = 6.1
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 2/42 (4%)
Frame = +1
Query: 106 AVALCSHLFWKGTRDGRPV--WALNYASRALDCLKKAARVAQ 225
A L +W+GT RPV W LN R D +++A +AQ
Sbjct: 76 AAGLVDPAYWRGTLRPRPVLDWYLN---RIDDAVREANELAQ 114
>At3g61240.2 68416.m06854 DEAD/DEAH box helicase, putative (RH12)
identical to cDNA DEAD box RNA helicase, RH12 GI:3776000
Length = 498
Score = 25.8 bits (54), Expect = 6.1
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -2
Query: 255 VRLHTAVHALLGHAGRLLQAVQR 187
+RLH VH L+G GR+L ++
Sbjct: 237 MRLHQPVHLLVGTPGRILDLTKK 259
>At3g61240.1 68416.m06853 DEAD/DEAH box helicase, putative (RH12)
identical to cDNA DEAD box RNA helicase, RH12 GI:3776000
Length = 498
Score = 25.8 bits (54), Expect = 6.1
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -2
Query: 255 VRLHTAVHALLGHAGRLLQAVQR 187
+RLH VH L+G GR+L ++
Sbjct: 237 MRLHQPVHLLVGTPGRILDLTKK 259
>At3g60740.1 68416.m06795 tubulin folding cofactor D identical to
tubulin folding cofactor D GI:20514263 from [Arabidopsis
thaliana]
Length = 1254
Score = 25.4 bits (53), Expect = 8.1
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 34 NAEPMRTQCALAASKLLKKPDQSRA 108
+A PMR L SKLL +PD +A
Sbjct: 228 SAGPMRRISRLLLSKLLTRPDMGKA 252
>At1g68500.1 68414.m07825 expressed protein
Length = 93
Score = 25.4 bits (53), Expect = 8.1
Identities = 15/47 (31%), Positives = 19/47 (40%), Gaps = 1/47 (2%)
Frame = +1
Query: 136 KGTRDGRPVWALNYASRALDCLKKAARVAQQCMDGG-VQAHCWPSCW 273
+GT D + A A AA +QC GG V+ W S W
Sbjct: 11 EGTADDHSLAAAAMAVAVAAAAATAAEEEEQCWGGGVVEEITWSSVW 57
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,374,183
Number of Sequences: 28952
Number of extensions: 83826
Number of successful extensions: 283
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 279
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 283
length of database: 12,070,560
effective HSP length: 71
effective length of database: 10,014,968
effective search space used: 330493944
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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