BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--1150 (611 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC12C2.07c |||spermidine synthase |Schizosaccharomyces pombe|c... 29 0.40 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 29 0.53 SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 27 2.8 SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k... 26 4.9 SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 8.6 >SPBC12C2.07c |||spermidine synthase |Schizosaccharomyces pombe|chr 2|||Manual Length = 298 Score = 29.5 bits (63), Expect = 0.40 Identities = 23/63 (36%), Positives = 31/63 (49%), Gaps = 5/63 (7%) Frame = +2 Query: 218 RGGPVPNSPYSECITIHWPSFYNVVTGKTLALPNL-IALQHIPLSPAG----VIAKRPAP 382 RGG V + +EC+ IH NV+T PN+ A IP P+G V+A + A Sbjct: 193 RGGGVITTQ-AECMWIHLGVISNVLTAVKTVFPNVRYAYTTIPTYPSGSIGFVVASKDAS 251 Query: 383 IAL 391 I L Sbjct: 252 IDL 254 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 29.1 bits (62), Expect = 0.53 Identities = 19/65 (29%), Positives = 31/65 (47%), Gaps = 5/65 (7%) Frame = +2 Query: 227 PVPNSPYSECITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSPAGVIAKRPAPIAL 391 P+P P ++ H + + +L N I+L ++PLSP A+ P+PI L Sbjct: 170 PLPRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRL 229 Query: 392 PNSCA 406 +S A Sbjct: 230 YSSDA 234 >SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 732 Score = 26.6 bits (56), Expect = 2.8 Identities = 12/25 (48%), Positives = 15/25 (60%) Frame = +1 Query: 340 PPFASWRNSEEARTDRPSQQLRSLN 414 PP AS RN E + PS+Q S+N Sbjct: 353 PPGASGRNRRERTSSTPSEQSTSVN 377 >SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 836 Score = 25.8 bits (54), Expect = 4.9 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = -3 Query: 600 SPRSLIVDSCSKLEQHSTLSRSILLI 523 SP +L +CS L HST + L+ Sbjct: 28 SPNNLTEQTCSPLRAHSTFKEPVFLL 53 >SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 25.0 bits (52), Expect = 8.6 Identities = 15/34 (44%), Positives = 19/34 (55%), Gaps = 1/34 (2%) Frame = +3 Query: 144 LNQQLDDFKTPASV-QCVYNPTKYARGGARYPIR 242 L Q L+ +TP V + YNPTK+ G Y IR Sbjct: 493 LIQNLNSPRTPKIVGKMRYNPTKHCWEGNDYAIR 526 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,690,494 Number of Sequences: 5004 Number of extensions: 55839 Number of successful extensions: 119 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 118 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 119 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 267622334 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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