BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--1147 (584 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 148 5e-37 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 67 2e-12 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 63 3e-11 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 62 4e-11 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 47 2e-06 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 39 6e-04 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 38 0.001 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 38 0.001 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 33 0.023 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.2 SPAC22A12.09c |sap114||splicing factor Sap114|Schizosaccharomyce... 27 1.5 SPBC11B10.02c |his3||histidinol-phosphate aminotransferase imida... 27 1.5 SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 4.7 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 26 4.7 SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pom... 25 8.1 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 25 8.1 SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz... 25 8.1 SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 25 8.1 SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc... 25 8.1 SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 25 8.1 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 148 bits (359), Expect = 5e-37 Identities = 67/73 (91%), Positives = 71/73 (97%) Frame = -2 Query: 508 TTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEY 329 TTMYPGIADRMQKEI ALAPS+MK+KI+APPERKYSVWIGGSILASLSTFQQMWISKQEY Sbjct: 303 TTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEY 362 Query: 328 DESGPSIVHRKCF 290 DESGP IV+RKCF Sbjct: 363 DESGPGIVYRKCF 375 Score = 52.4 bits (120), Expect = 5e-08 Identities = 22/25 (88%), Positives = 23/25 (92%) Frame = -3 Query: 582 TYNSIMKCDVDIRKDLYANTVLSGG 508 TYNSIMKCDVDIRKDLY N V+SGG Sbjct: 278 TYNSIMKCDVDIRKDLYGNVVMSGG 302 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 66.9 bits (156), Expect = 2e-12 Identities = 33/78 (42%), Positives = 53/78 (67%), Gaps = 6/78 (7%) Frame = -2 Query: 508 TTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILASLSTFQQMWISK 338 T++ G + R+Q E++ L P + ++KI A ER Y+ W+GGSIL+SL TF Q+WIS+ Sbjct: 356 TSLMQGFSLRLQNELSKLYPGS-RLKIHASGHVVERSYASWLGGSILSSLGTFHQLWISR 414 Query: 337 QEYDESGP---SIVHRKC 293 QEY+E G +++ ++C Sbjct: 415 QEYEEHGSDRLALIEKRC 432 Score = 29.9 bits (64), Expect = 0.29 Identities = 12/23 (52%), Positives = 15/23 (65%) Frame = -3 Query: 576 NSIMKCDVDIRKDLYANTVLSGG 508 N I +CDVDIR L N ++ GG Sbjct: 333 NCISECDVDIRASLLNNVIVCGG 355 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 63.3 bits (147), Expect = 3e-11 Identities = 28/73 (38%), Positives = 50/73 (68%) Frame = -2 Query: 508 TTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEY 329 +T+ G +R E+ A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ +EY Sbjct: 308 STLLRGFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY 367 Query: 328 DESGPSIVHRKCF 290 ++ +++ R+ F Sbjct: 368 -KNDQNVIFRRRF 379 Score = 33.1 bits (72), Expect = 0.031 Identities = 13/24 (54%), Positives = 18/24 (75%) Frame = -3 Query: 579 YNSIMKCDVDIRKDLYANTVLSGG 508 + SI+ D+D+R LY+N VLSGG Sbjct: 284 FKSILLSDIDLRSTLYSNIVLSGG 307 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 62.5 bits (145), Expect = 4e-11 Identities = 30/66 (45%), Positives = 42/66 (63%), Gaps = 2/66 (3%) Frame = -2 Query: 508 TTMYPGIADRMQKEITALAP-STMKIKIIAPPER-KYSVWIGGSILASLSTFQQMWISKQ 335 T++ PG+++R+Q E+ LA S + + +VW GGSILASL FQ +W+SKQ Sbjct: 353 TSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLDNFQHLWVSKQ 412 Query: 334 EYDESG 317 EYDE G Sbjct: 413 EYDEVG 418 Score = 28.7 bits (61), Expect = 0.66 Identities = 11/24 (45%), Positives = 16/24 (66%) Frame = -3 Query: 579 YNSIMKCDVDIRKDLYANTVLSGG 508 Y SI+ CD ++R L N V++GG Sbjct: 329 YQSILACDSELRSPLLNNIVVTGG 352 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 46.8 bits (106), Expect = 2e-06 Identities = 27/76 (35%), Positives = 46/76 (60%), Gaps = 12/76 (15%) Frame = -2 Query: 508 TTMYPGIADRMQKEITAL--------APSTM---KIKIIAPPERKYSVWIGGSILAS-LS 365 ++MY G+ R++KEI L P+ + K+KI P R+++V+IGG++LA ++ Sbjct: 303 SSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADIMA 362 Query: 364 TFQQMWISKQEYDESG 317 MW+SK E++E G Sbjct: 363 QNDHMWVSKAEWEEYG 378 Score = 30.7 bits (66), Expect = 0.16 Identities = 14/24 (58%), Positives = 17/24 (70%) Frame = -3 Query: 579 YNSIMKCDVDIRKDLYANTVLSGG 508 +++I DVDIRK LY VLSGG Sbjct: 279 FDTIQAADVDIRKYLYRAIVLSGG 302 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 38.7 bits (86), Expect = 6e-04 Identities = 20/68 (29%), Positives = 35/68 (51%) Frame = -2 Query: 499 YPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDES 320 +PG R+ E+ +LAP+ ++K+ P + W S + L + I++ EY E Sbjct: 332 FPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEH 390 Query: 319 GPSIVHRK 296 G +I+ RK Sbjct: 391 GANIMTRK 398 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 37.9 bits (84), Expect = 0.001 Identities = 16/64 (25%), Positives = 31/64 (48%) Frame = -2 Query: 496 PGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESG 317 PG+ R+++E+T++ P I + W G S + F+ ++++EY E G Sbjct: 648 PGMETRIKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKG 707 Query: 316 PSIV 305 P + Sbjct: 708 PEYI 711 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 37.5 bits (83), Expect = 0.001 Identities = 16/46 (34%), Positives = 26/46 (56%) Frame = -2 Query: 436 IKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 299 + +I+ ++ +VW GGS+LA F +K +Y+E G SI R Sbjct: 374 VNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419 Score = 30.3 bits (65), Expect = 0.22 Identities = 12/23 (52%), Positives = 15/23 (65%) Frame = -3 Query: 576 NSIMKCDVDIRKDLYANTVLSGG 508 N + +D+RK LY N VLSGG Sbjct: 313 NVVQSSPIDVRKGLYKNIVLSGG 335 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 33.5 bits (73), Expect = 0.023 Identities = 17/78 (21%), Positives = 34/78 (43%), Gaps = 3/78 (3%) Frame = -2 Query: 520 IVRWTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILASLSTFQQM 350 IV +PG A +++ I + + I +I PP + ++ W G I + ++ Sbjct: 535 IVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYNRIRIVSEL 594 Query: 349 WISKQEYDESGPSIVHRK 296 WI ++ G ++ K Sbjct: 595 WIKNSDWKMLGSRVLQYK 612 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 1.2 Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%) Frame = -2 Query: 397 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 296 ++GGSI+A S + + +++ +EY + GP+ +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPAC22A12.09c |sap114||splicing factor Sap114|Schizosaccharomyces pombe|chr 1|||Manual Length = 481 Score = 27.5 bits (58), Expect = 1.5 Identities = 17/46 (36%), Positives = 24/46 (52%) Frame = -3 Query: 381 SSPPSLPSNKCGSRNKSTTSLAPPLYTGSASKRTARRCLQQPGPAA 244 S PPSL S + G + STTS++P T + +Q+P P A Sbjct: 332 SLPPSLASPEKGGIS-STTSVSPAAQASPVLSTTTQPKVQKPVPKA 376 >SPBC11B10.02c |his3||histidinol-phosphate aminotransferase imidazole acetol phosphate transaminase His3|Schizosaccharomyces pombe|chr 2|||Manual Length = 384 Score = 27.5 bits (58), Expect = 1.5 Identities = 15/37 (40%), Positives = 19/37 (51%) Frame = +2 Query: 452 GESCDFLLHTVGDSRVHGGPPDNTVLAYKSLRMSTSH 562 G +F+L V D R GG P N Y L+M+T H Sbjct: 304 GYDANFILIQVLD-RPEGGKPSNDAAKYLYLQMATMH 339 >SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 412 Score = 25.8 bits (54), Expect = 4.7 Identities = 12/47 (25%), Positives = 26/47 (55%) Frame = -3 Query: 339 NKSTTSLAPPLYTGSASKRTARRCLQQPGPAAQFRLVISVNLIVRKI 199 N+S +SL+ Y+ SK+T+ L + + Q +++ I+R++ Sbjct: 241 NRSGSSLSSNFYSSRLSKKTSLASLNKSRASLQHKIMSLSRNIIRRV 287 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 25.8 bits (54), Expect = 4.7 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = -2 Query: 472 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 362 KEI++ A S + + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 204 Score = 25.0 bits (52), Expect = 8.1 Identities = 20/56 (35%), Positives = 26/56 (46%), Gaps = 5/56 (8%) Frame = -3 Query: 402 PYGSVDRSSPPSLP----SNKCG-SRNKSTTSLAPPLYTGSASKRTARRCLQQPGP 250 P SVD S+PPS P SN + ++ S A P +AS+ A QP P Sbjct: 49 PPPSVDHSAPPSGPPPSYSNSAAPATPAASASSAAPAPAPAASQNRAYGAAPQPYP 104 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 25.0 bits (52), Expect = 8.1 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = -3 Query: 405 TPYGSVDRSSPPSLPSNKCGSRNKSTTSLAPP 310 TP + PPS+PS++ R S ++ APP Sbjct: 223 TPTSTSAPPIPPSIPSSRPPERVPSLSAPAPP 254 >SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schizosaccharomyces pombe|chr 2|||Manual Length = 628 Score = 25.0 bits (52), Expect = 8.1 Identities = 7/10 (70%), Positives = 8/10 (80%) Frame = -1 Query: 542 RTCTPTPYCP 513 +TC P PYCP Sbjct: 422 KTCKPNPYCP 431 >SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pombe|chr 1||Partial|Manual Length = 1887 Score = 25.0 bits (52), Expect = 8.1 Identities = 13/31 (41%), Positives = 16/31 (51%), Gaps = 2/31 (6%) Frame = -3 Query: 399 YGSVDRSSPPSLPSNKCG--SRNKSTTSLAP 313 Y + SSPP P N G + N +TTS P Sbjct: 1613 YNASFSSSPPPQPGNSSGMSAMNTNTTSTTP 1643 >SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1284 Score = 25.0 bits (52), Expect = 8.1 Identities = 11/29 (37%), Positives = 15/29 (51%) Frame = +2 Query: 338 FRDPHLLEGREGGEDRSTDPYGVLPLWRS 424 F HLL + G+D + + YG L W S Sbjct: 797 FHQGHLLYLSDEGKDETLERYGTLSSWIS 825 >SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1919 Score = 25.0 bits (52), Expect = 8.1 Identities = 13/31 (41%), Positives = 16/31 (51%), Gaps = 2/31 (6%) Frame = -3 Query: 399 YGSVDRSSPPSLPSNKCG--SRNKSTTSLAP 313 Y + SSPP P N G + N +TTS P Sbjct: 1613 YNASFSSSPPPQPGNSSGMSAMNTNTTSTTP 1643 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,334,829 Number of Sequences: 5004 Number of extensions: 46753 Number of successful extensions: 158 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 147 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 154 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 252150250 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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