BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--1145 (600 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g07910.1 68414.m00860 expressed protein identical to GB:AAB07... 35 0.047 At5g16570.1 68418.m01939 glutamine synthetase, putative similar ... 33 0.11 At1g66200.1 68414.m07514 glutamine synthetase, putative similar ... 33 0.11 At3g49490.1 68416.m05409 expressed protein 32 0.25 At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to... 32 0.25 At5g37600.1 68418.m04529 glutamine synthetase, putative similar ... 30 1.0 At5g56420.2 68418.m07043 F-box family protein contains F-box dom... 25 1.7 At5g56420.1 68418.m07042 F-box family protein contains F-box dom... 25 1.7 At1g34060.1 68414.m04222 alliinase family protein contains Pfam ... 29 1.8 At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to... 29 3.1 At2g27930.1 68415.m03385 zinc-binding family protein similar to ... 28 5.4 At5g64550.1 68418.m08112 loricrin-related contains weak similari... 27 7.2 At5g13655.1 68418.m01588 hypothetical protein 27 9.5 >At1g07910.1 68414.m00860 expressed protein identical to GB:AAB07881 AT.I.24-9 gene product from [Arabidopsis thaliana] (Mol. Gen. Genet. 219 (1-2), 106-112 (1989)) Length = 1081 Score = 34.7 bits (76), Expect = 0.047 Identities = 20/65 (30%), Positives = 30/65 (46%) Frame = +2 Query: 245 IRYGPAPPCVAATFEQSDPPRDIIDAHKSNKQNGMIKLKGTLAREVASAWECRVGPPDHP 424 +R P PPC A + R+I AH+SN++ M L ++ + G HP Sbjct: 322 LRDHPPPPCDGANLDLGLSLREICAAHRSNEKQQMRALLRSVGPSFCPSDVEWFGDESHP 381 Query: 425 ASADR 439 SAD+ Sbjct: 382 KSADK 386 >At5g16570.1 68418.m01939 glutamine synthetase, putative similar to glutamine synthetase, cytosolic isozyme (glutamate-- ammonia ligase) [Alfalfa] SWISS-PROT:P04078 Length = 356 Score = 33.5 bits (73), Expect = 0.11 Identities = 19/63 (30%), Positives = 27/63 (42%) Frame = +2 Query: 254 GPAPPCVAATFEQSDPPRDIIDAHKSNKQNGMIKLKGTLAREVASAWECRVGPPDHPASA 433 GP P RDI+D+H I + GT + WE +VGP A+A Sbjct: 152 GPQGPYYCGVGADKAFGRDIVDSHYKACLYAGINVSGTNGEVMPGQWEFQVGPTVGIAAA 211 Query: 434 DRI 442 D++ Sbjct: 212 DQV 214 >At1g66200.1 68414.m07514 glutamine synthetase, putative similar to glutamine synthetase, cytosolic isozyme (Glutamate-- ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899 Length = 356 Score = 33.5 bits (73), Expect = 0.11 Identities = 19/63 (30%), Positives = 26/63 (41%) Frame = +2 Query: 254 GPAPPCVAATFEQSDPPRDIIDAHKSNKQNGMIKLKGTLAREVASAWECRVGPPDHPASA 433 GP P + RDI+DAH I + G + WE +VGP ++A Sbjct: 152 GPQGPYYCSIGADKSFGRDIVDAHYKASLYAGINISGINGEVMPGQWEFQVGPSVGISAA 211 Query: 434 DRI 442 D I Sbjct: 212 DEI 214 >At3g49490.1 68416.m05409 expressed protein Length = 953 Score = 32.3 bits (70), Expect = 0.25 Identities = 17/37 (45%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Frame = +2 Query: 410 PPDHPASADRIQSLFNDIELYPTKTQVFE-MLVCHDS 517 PP HPASA Q N +E Y +VF+ M++ HDS Sbjct: 27 PPHHPASAPPFQQ-SNPVESYDPAKEVFDPMMLYHDS 62 >At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to glutamine synthetase, cytosolic isozyme (glutamate-- ammonia ligase, GS1) [Arabidopsis thaliana] SWISS-PROT:Q9LVI8 Length = 354 Score = 32.3 bits (70), Expect = 0.25 Identities = 18/63 (28%), Positives = 26/63 (41%) Frame = +2 Query: 254 GPAPPCVAATFEQSDPPRDIIDAHKSNKQNGMIKLKGTLAREVASAWECRVGPPDHPASA 433 GP P RDI+DAH I + G + WE +VGP + +S Sbjct: 152 GPQGPYYCGVGADKAIGRDIVDAHYKACLYAGIGISGINGEVMPGQWEFQVGPVEGISSG 211 Query: 434 DRI 442 D++ Sbjct: 212 DQV 214 >At5g37600.1 68418.m04529 glutamine synthetase, putative similar to glutamine synthetase, cytosolic isozyme (Glutamate-- ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899 Length = 356 Score = 30.3 bits (65), Expect = 1.0 Identities = 17/63 (26%), Positives = 25/63 (39%) Frame = +2 Query: 254 GPAPPCVAATFEQSDPPRDIIDAHKSNKQNGMIKLKGTLAREVASAWECRVGPPDHPASA 433 GP P RD++D+H I + G + WE +VGP ++A Sbjct: 152 GPQGPYYCGIGADKSFGRDVVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAA 211 Query: 434 DRI 442 D I Sbjct: 212 DEI 214 >At5g56420.2 68418.m07043 F-box family protein contains F-box domain Pfam:PF00646 Length = 422 Score = 24.6 bits (51), Expect(2) = 1.7 Identities = 10/23 (43%), Positives = 15/23 (65%) Frame = -2 Query: 560 QNSPNVRVSDLRRAHCRGIPASR 492 ++SP +RV LR+ H R P+ R Sbjct: 318 EDSPKLRVLKLRQKHIRRAPSPR 340 Score = 23.4 bits (48), Expect(2) = 1.7 Identities = 10/19 (52%), Positives = 10/19 (52%) Frame = -2 Query: 416 REAPRGTPKRSQPPLPKCL 360 R AP QP LPKCL Sbjct: 334 RRAPSPRASWKQPALPKCL 352 >At5g56420.1 68418.m07042 F-box family protein contains F-box domain Pfam:PF00646 Length = 422 Score = 24.6 bits (51), Expect(2) = 1.7 Identities = 10/23 (43%), Positives = 15/23 (65%) Frame = -2 Query: 560 QNSPNVRVSDLRRAHCRGIPASR 492 ++SP +RV LR+ H R P+ R Sbjct: 318 EDSPKLRVLKLRQKHIRRAPSPR 340 Score = 23.4 bits (48), Expect(2) = 1.7 Identities = 10/19 (52%), Positives = 10/19 (52%) Frame = -2 Query: 416 REAPRGTPKRSQPPLPKCL 360 R AP QP LPKCL Sbjct: 334 RRAPSPRASWKQPALPKCL 352 >At1g34060.1 68414.m04222 alliinase family protein contains Pfam profiles: PF04864 allinase C-terminal domain, PF04863 alliinase EGF-like domain Length = 463 Score = 29.5 bits (63), Expect = 1.8 Identities = 12/30 (40%), Positives = 15/30 (50%) Frame = +1 Query: 229 SRVAWNPLWSSAAVCRRDVRAVGSTSGHHR 318 S WNP W++ A + A S SGH R Sbjct: 26 SSTTWNPTWTNRAAAEAETVASFSCSGHGR 55 >At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to glutamine synthetase, chloroplast precursor (glutamate-- ammonia ligase, GS2) [Arabidopsis thaliana] SWISS-PROT:Q43127 Length = 430 Score = 28.7 bits (61), Expect = 3.1 Identities = 17/53 (32%), Positives = 20/53 (37%) Frame = +2 Query: 254 GPAPPCVAATFEQSDPPRDIIDAHKSNKQNGMIKLKGTLAREVASAWECRVGP 412 GP P RDI DAH I + GT + WE +VGP Sbjct: 210 GPQGPYYCGVGADKIWGRDISDAHYKACLYAGINISGTNGEVMPGQWEFQVGP 262 >At2g27930.1 68415.m03385 zinc-binding family protein similar to zinc-binding protein [Pisum sativum] GI:16117799; contains Pfam profile PF04640 : Protein of unknown function, DUF597 Length = 135 Score = 27.9 bits (59), Expect = 5.4 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = -3 Query: 373 CQSAFQFDHSILLIRLVSIDDVPR 302 C+S+F DH +L IR S DV R Sbjct: 46 CRSSFHIDHPVLQIRRSSYHDVVR 69 >At5g64550.1 68418.m08112 loricrin-related contains weak similarity to Loricrin (Swiss-Prot:P23490) [Homo sapiens] Length = 634 Score = 27.5 bits (58), Expect = 7.2 Identities = 9/26 (34%), Positives = 16/26 (61%) Frame = +1 Query: 364 HFGKGGCERLGVPRGASRSSSICRSH 441 H G C++LG +GA ++ C++H Sbjct: 235 HGGGQRCQKLGCNKGAESKTTFCKAH 260 >At5g13655.1 68418.m01588 hypothetical protein Length = 395 Score = 27.1 bits (57), Expect = 9.5 Identities = 12/33 (36%), Positives = 21/33 (63%), Gaps = 4/33 (12%) Frame = +1 Query: 367 FGKGGCERLGVPRGASRSSSIC----RSHPEPI 453 FG+ GC +G+P+ ++ SS+I R H +P+ Sbjct: 140 FGERGCIDMGIPKDSTLSSAIMTTRRRKHRQPL 172 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,775,670 Number of Sequences: 28952 Number of extensions: 293361 Number of successful extensions: 765 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 742 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 765 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1187288784 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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