BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--1130 (659 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 27 3.2 SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 27 3.2 SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog Rhp16|Schizo... 26 4.2 SPAC6G10.12c |ace2||transcription factor Ace2|Schizosaccharomyce... 26 5.5 SPBC543.04 |||UPF0171 family protein|Schizosaccharomyces pombe|c... 26 5.5 SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c... 26 5.5 SPCC162.01c |||RNA-binding protein |Schizosaccharomyces pombe|ch... 25 9.7 >SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 732 Score = 26.6 bits (56), Expect = 3.2 Identities = 12/25 (48%), Positives = 15/25 (60%) Frame = +1 Query: 409 PPFASWRNSEEARTDRPSQQLRSLN 483 PP AS RN E + PS+Q S+N Sbjct: 353 PPGASGRNRRERTSSTPSEQSTSVN 377 >SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosaccharomyces pombe|chr 1|||Manual Length = 155 Score = 26.6 bits (56), Expect = 3.2 Identities = 9/11 (81%), Positives = 11/11 (100%) Frame = +2 Query: 185 DSLFYWACTAL 217 D+LF+WACTAL Sbjct: 34 DNLFHWACTAL 44 >SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog Rhp16|Schizosaccharomyces pombe|chr 3|||Manual Length = 963 Score = 26.2 bits (55), Expect = 4.2 Identities = 12/36 (33%), Positives = 16/36 (44%) Frame = -1 Query: 278 HAVPGPADWWTPSPTCSATKSVQCKPSRTGNLHKQI 171 H VPG D W+ SP + + NLH Q+ Sbjct: 68 HDVPGNQDEWSSSPIAGIIVESEDEEVVENNLHSQM 103 >SPAC6G10.12c |ace2||transcription factor Ace2|Schizosaccharomyces pombe|chr 1|||Manual Length = 533 Score = 25.8 bits (54), Expect = 5.5 Identities = 16/71 (22%), Positives = 35/71 (49%) Frame = +1 Query: 355 RRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWQIVSVIFC*NSR*I 534 +RD+E G ++ ++ PF + + +A P L++ ++ SV+ + + Sbjct: 30 QRDYETFGDSKHTSFSSFAPFENEVSLNQAVIPSPPSHLQNFQDQFDYSSVLKTPFNPLL 89 Query: 535 FVKSAHFLTNR 567 SA+FL+N+ Sbjct: 90 EANSAYFLSNQ 100 >SPBC543.04 |||UPF0171 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 585 Score = 25.8 bits (54), Expect = 5.5 Identities = 11/36 (30%), Positives = 22/36 (61%) Frame = -3 Query: 534 NLTRILTKYNAYNLPFAIQAAQLLGRAIGAGLFAIT 427 ++ ++ KY++ N+PF + AAQ+ + A + A T Sbjct: 217 HILKLTEKYSSQNVPFDVYAAQIPHHSNLASVLATT 252 >SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 871 Score = 25.8 bits (54), Expect = 5.5 Identities = 13/33 (39%), Positives = 19/33 (57%) Frame = +1 Query: 133 IKYVQIIRTLLIYICLWRFPVLLGLHCTDFVAE 231 I+Y+ L I CL FP+LL ++ T+ V E Sbjct: 90 IRYLFTFGALCILGCLVLFPILLPVNATNGVGE 122 >SPCC162.01c |||RNA-binding protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 244 Score = 25.0 bits (52), Expect = 9.7 Identities = 18/58 (31%), Positives = 25/58 (43%) Frame = -2 Query: 346 RRPVNCNTTHYRANWVPGPPSRRTRCPDPPTGGRRHQRARQQNQCSASPVEQGISTSK 173 RRPV H R NW P R R + GRR ++ R+ SP +G+ + Sbjct: 79 RRPVE----HER-NWQPELKHGRERFRERDYEGRRDRKERRDGVSPFSPEGEGLERKR 131 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,631,896 Number of Sequences: 5004 Number of extensions: 53869 Number of successful extensions: 127 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 123 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 127 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 299817502 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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