Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--1066
(522 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putati... 107 3e-24
At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putati... 106 8e-24
At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putati... 103 6e-23
At2g11270.1 68415.m01208 citrate synthase-related contains simil... 44 6e-05
At4g16442.1 68417.m02489 integral membrane family protein contai... 29 1.4
At5g41770.1 68418.m05086 crooked neck protein, putative / cell c... 28 3.3
At5g32605.1 68418.m03879 hypothetical protein 28 3.3
At2g22010.1 68415.m02614 zinc finger (C3HC4-type RING finger) fa... 28 3.3
At5g52280.1 68418.m06488 protein transport protein-related low s... 27 5.8
At5g50860.1 68418.m06302 protein kinase family protein contains ... 27 5.8
At5g28300.1 68418.m03436 trihelix DNA-binding protein, putative ... 27 5.8
At3g51150.1 68416.m05601 kinesin motor family protein contains P... 27 5.8
At3g32190.1 68416.m04102 hypothetical protein 27 7.7
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 27 7.7
At1g76950.1 68414.m08958 zinc finger protein (PRAF1) / regulator... 27 7.7
At1g08840.1 68414.m00984 DNA replication helicase, putative simi... 27 7.7
>At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 474
Score = 107 bits (258), Expect = 3e-24
Identities = 48/96 (50%), Positives = 72/96 (75%), Gaps = 3/96 (3%)
Frame = +1
Query: 220 RIAESMSDCDSSLRGL---SAEQTNLKSILQEKIPKEQEKIREFRKKHGSTKVGEVTVDM 390
R+ S +S+R + S+ +LKS LQE IP++Q+++++ + +HG ++G +TVDM
Sbjct: 16 RVQGQQSSLSNSVRWIQMQSSTDLDLKSQLQELIPEQQDRLKKLKSEHGKVQLGNITVDM 75
Query: 391 MYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQ 498
+ GGMRG+ GL+WETS+LD +EGIRFRGLSIPECQ+
Sbjct: 76 VIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQK 111
>At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 473
Score = 106 bits (255), Expect = 8e-24
Identities = 46/89 (51%), Positives = 70/89 (78%)
Frame = +1
Query: 232 SMSDCDSSLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGSTKVGEVTVDMMYGGMRG 411
S+S+ ++ S+ +LKS LQE IP++Q+++++ + +HG ++G +TVDM+ GGMRG
Sbjct: 22 SLSNSVRWIQMQSSTDLDLKSQLQELIPEQQDRLKKLKSEHGKVQLGNITVDMVIGGMRG 81
Query: 412 IKGLVWETSVLDADEGIRFRGLSIPECQQ 498
+ GL+WETS+LD +EGIRFRGLSIPECQ+
Sbjct: 82 MTGLLWETSLLDPEEGIRFRGLSIPECQK 110
>At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putative
strong similarity to SP|Q43175 Citrate synthase,
mitochondrial precursor {Solanum tuberosum}; contains
Pfam profile PF00285: Citrate synthase
Length = 433
Score = 103 bits (248), Expect = 6e-23
Identities = 43/72 (59%), Positives = 61/72 (84%)
Frame = +1
Query: 283 NLKSILQEKIPKEQEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGLVWETSVLDADEGI 462
+LKS +QE IP++Q+++++ + + G VG +TVDM+ GGMRG+ GL+WETS+LDADEGI
Sbjct: 5 DLKSQMQEIIPEQQDRLKKLKSEQGKVPVGNITVDMVLGGMRGMTGLLWETSLLDADEGI 64
Query: 463 RFRGLSIPECQQ 498
RFRG+SIPECQ+
Sbjct: 65 RFRGMSIPECQK 76
>At2g11270.1 68415.m01208 citrate synthase-related contains
similarity to Swiss-Prot:P20115 citrate synthase,
mitochondrial precursor [Arabidopsis thaliana]
Length = 83
Score = 44.0 bits (99), Expect = 6e-05
Identities = 17/34 (50%), Positives = 25/34 (73%)
Frame = +1
Query: 322 QEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGL 423
Q++ ++ + KHG VG +TVDM+ GGMRG+ GL
Sbjct: 43 QDRSKKLKLKHGKVPVGNITVDMVLGGMRGMTGL 76
>At4g16442.1 68417.m02489 integral membrane family protein contains
TIGRFAM TIGR01569 : plant integral membrane protein
TIGR01569; contains Pfam PF04535 : Domain of unknown
function (DUF588)
Length = 182
Score = 29.5 bits (63), Expect = 1.4
Identities = 14/46 (30%), Positives = 22/46 (47%)
Frame = +2
Query: 380 QLI*CTVACVESKVWFGKPLCWMPMKESVSVVYPSLSANKAKPAQG 517
Q + C V ++ KV F KPL W ++ Y +++A A G
Sbjct: 71 QSVRCVVGTMKGKVLFSKPLAWAFFSGDQAMAYLNVAAIAATAESG 116
>At5g41770.1 68418.m05086 crooked neck protein, putative / cell
cycle protein, putative similar to Swiss-Prot:P17886
crooked neck protein [Drosophila melanogaster]
Length = 705
Score = 28.3 bits (60), Expect = 3.3
Identities = 13/35 (37%), Positives = 23/35 (65%)
Frame = +1
Query: 292 SILQEKIPKEQEKIREFRKKHGSTKVGEVTVDMMY 396
SI+Q K+PK+ +K + ++ GST+ E +D +Y
Sbjct: 643 SIVQSKLPKKLKKRKAITREDGSTEYEEY-IDYLY 676
>At5g32605.1 68418.m03879 hypothetical protein
Length = 365
Score = 28.3 bits (60), Expect = 3.3
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +1
Query: 217 RRIAESMSDCDSSLRGLSAEQTNLKS 294
R+I+ S DCD+ + LS + TNL S
Sbjct: 182 RQISSSDKDCDAKIEALSDKDTNLVS 207
>At2g22010.1 68415.m02614 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 1280
Score = 28.3 bits (60), Expect = 3.3
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = -3
Query: 508 GFGLVGTQGWIDHGNGFLHRHPAQRFP 428
GF ++ + + H GFLHR Q+FP
Sbjct: 567 GFAMLKSSEAVHHNVGFLHRGGQQKFP 593
>At5g52280.1 68418.m06488 protein transport protein-related low
similarity to SP|P25386 Intracellular protein transport
protein USO1 {Saccharomyces cerevisiae}
Length = 853
Score = 27.5 bits (58), Expect = 5.8
Identities = 12/23 (52%), Positives = 13/23 (56%), Gaps = 1/23 (4%)
Frame = -3
Query: 511 GGFGLVGTQGWIDHGNGFL-HRH 446
G F +G GWID GN L RH
Sbjct: 192 GSFDSIGESGWIDDGNARLPQRH 214
>At5g50860.1 68418.m06302 protein kinase family protein contains
PF00069: Protein kinase domain
Length = 580
Score = 27.5 bits (58), Expect = 5.8
Identities = 22/84 (26%), Positives = 38/84 (45%)
Frame = +1
Query: 223 IAESMSDCDSSLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGSTKVGEVTVDMMYGG 402
+ E++ D + RG S N + E +P + + K+ +K E+ V +
Sbjct: 374 LVETLLTIDPADRGTSTSALNSEFFTTEPLPCDPSSL----PKYPPSK--ELNVKLRDEE 427
Query: 403 MRGIKGLVWETSVLDADEGIRFRG 474
+R KGL + S +D IR+RG
Sbjct: 428 LRRQKGLAGKGSGIDGARRIRYRG 451
>At5g28300.1 68418.m03436 trihelix DNA-binding protein, putative
similar to GT-2 factor [Arabidopsis thaliana GI:416490
Length = 619
Score = 27.5 bits (58), Expect = 5.8
Identities = 11/31 (35%), Positives = 21/31 (67%), Gaps = 1/31 (3%)
Frame = +1
Query: 244 CDSSLRGLSAEQTNL-KSILQEKIPKEQEKI 333
C+ +R + A+Q + K +L++ + KE+EKI
Sbjct: 303 CEGLVRNMIAQQEEMHKKLLEDMVKKEEEKI 333
>At3g51150.1 68416.m05601 kinesin motor family protein contains Pfam
domain, PF00225: Kinesin motor domain
Length = 1025
Score = 27.5 bits (58), Expect = 5.8
Identities = 11/27 (40%), Positives = 19/27 (70%)
Frame = +1
Query: 250 SSLRGLSAEQTNLKSILQEKIPKEQEK 330
SSLR L+ E+ L ++Q+K+ KE+ +
Sbjct: 916 SSLRALNRERYKLSQLMQKKLTKEERE 942
>At3g32190.1 68416.m04102 hypothetical protein
Length = 358
Score = 27.1 bits (57), Expect = 7.7
Identities = 16/58 (27%), Positives = 26/58 (44%)
Frame = +1
Query: 184 NGSIQDHIFKTRRIAESMSDCDSSLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHG 357
NG +QD + +I E +S+ L + +L + L + K K R+ K HG
Sbjct: 112 NGELQDQYQRYDKIQEELSNARGRLSESESNAYDLSNQLSKLQAKSASKARKEVKGHG 169
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 27.1 bits (57), Expect = 7.7
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -2
Query: 479 DRPRKRIPSSASSTEVSQTRPLIPRMP 399
DR R+R PSS+ S S++ P++ R P
Sbjct: 604 DRRRRRSPSSSRSPSRSRSPPVLHRSP 630
>At1g76950.1 68414.m08958 zinc finger protein (PRAF1) / regulator of
chromosome condensation (RCC1) family protein identical
to zinc finger protein PRAF1 [Arabidopsis thaliana]
gi|15811367|gb|AAL08940.
Length = 1103
Score = 27.1 bits (57), Expect = 7.7
Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 3/50 (6%)
Frame = -2
Query: 494 WHSG---MDRPRKRIPSSASSTEVSQTRPLIPRMPPYIISTVTSPTLVEP 354
W G +D R+ SS SS+ S +R P+ I +TSP EP
Sbjct: 136 WSGGGLSVDASRELTSSSPSSSSASASRGHSSPGTPFNIDPITSPKSAEP 185
>At1g08840.1 68414.m00984 DNA replication helicase, putative similar
to helicase [Xenopus laevis] gi|18845092|gb|AAL79550
Length = 1296
Score = 27.1 bits (57), Expect = 7.7
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = -3
Query: 373 LRL*WNHASFGIRGSSLVLWESFPGEYFLNSSVQRLDL 260
LRL W+ S + S LW + E+ + SV R +L
Sbjct: 807 LRLPWSEPSSEVSNLSHELWRIYKDEFMTSFSVMRFNL 844
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,163,106
Number of Sequences: 28952
Number of extensions: 221414
Number of successful extensions: 813
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 785
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 813
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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