BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--1050 (593 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g02590.1 68416.m00250 delta 7-sterol-C5-desaturase, putative ... 29 3.1 At3g07570.1 68416.m00907 membrane protein, putative similar to m... 28 4.1 At3g02580.1 68416.m00249 delta 7-sterol-C5-desaturase (STE1) ide... 27 7.1 >At3g02590.1 68416.m00250 delta 7-sterol-C5-desaturase, putative similar to delta7 sterol C-5 desaturase GI:5031219 from [Arabidopsis thaliana] Length = 279 Score = 28.7 bits (61), Expect = 3.1 Identities = 14/24 (58%), Positives = 17/24 (70%) Frame = -3 Query: 435 SNYMKCF*IYYLKKNVYSIYKRSI 364 S ++ CF IYYLK NVY + K SI Sbjct: 57 SGFLWCFYIYYLKLNVY-VPKESI 79 >At3g07570.1 68416.m00907 membrane protein, putative similar to membrane protein SDR2 (GI:1747306) [Mus musculus] Length = 369 Score = 28.3 bits (60), Expect = 4.1 Identities = 13/36 (36%), Positives = 21/36 (58%) Frame = -1 Query: 110 VVIVKFVYILITY*INNKQTKNTFCNIRVELNELTY 3 V I+ FV I ++ +N +Q CN + LN+LT+ Sbjct: 6 VSIIIFVLIALSTIVNAQQAATDSCNSTLPLNDLTF 41 >At3g02580.1 68416.m00249 delta 7-sterol-C5-desaturase (STE1) identical to sterol-C5-desaturase GB:AAD12944 GI:4234768 from [Arabidopsis thaliana] Length = 281 Score = 27.5 bits (58), Expect = 7.1 Identities = 11/17 (64%), Positives = 13/17 (76%) Frame = -3 Query: 435 SNYMKCF*IYYLKKNVY 385 S ++ CF IYYLK NVY Sbjct: 56 SGFLWCFYIYYLKINVY 72 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,022,414 Number of Sequences: 28952 Number of extensions: 172023 Number of successful extensions: 298 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 295 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 298 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1180950720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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