BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--1046 (655 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizos... 26 5.5 SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ... 26 5.5 SPCC736.07c |||cell polarity protein |Schizosaccharomyces pombe|... 25 9.5 SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 25 9.5 >SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 732 Score = 25.8 bits (54), Expect = 5.5 Identities = 12/26 (46%), Positives = 19/26 (73%), Gaps = 2/26 (7%) Frame = +3 Query: 51 TYLYLRLNMHKY--YVKHLIVSIERF 122 +Y+Y +++ K Y KHLI+S+ERF Sbjct: 690 SYIYPHISVLKKFTYGKHLIMSVERF 715 >SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 500 Score = 25.8 bits (54), Expect = 5.5 Identities = 12/33 (36%), Positives = 19/33 (57%) Frame = +1 Query: 484 KVNKYPQRVRPQLARGQSQLHKKQVFIKYRCTM 582 K+ KY ++VR A GQ+ + K V + Y T+ Sbjct: 6 KMQKYDRQVRLWKAEGQNAIEKSHVCLLYANTV 38 >SPCC736.07c |||cell polarity protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 699 Score = 25.0 bits (52), Expect = 9.5 Identities = 17/64 (26%), Positives = 28/64 (43%), Gaps = 3/64 (4%) Frame = +1 Query: 79 TSIM*NTSLFLLKGLVVRRRHTLHKMNIDTFDTNK---MNTLTNIKRSKNGIRVTQNVFI 249 TS+ LF + R + K N + NK +N L N ++NG + TQN + Sbjct: 222 TSLFKKGFLFKKNNAMQNRTTSNIKSNASAIEKNKETILNALNNSTLNENGHQDTQNEVL 281 Query: 250 NQLI 261 ++ Sbjct: 282 RDIV 285 >SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 1462 Score = 25.0 bits (52), Expect = 9.5 Identities = 13/38 (34%), Positives = 23/38 (60%), Gaps = 1/38 (2%) Frame = +1 Query: 175 TNKMNTLTNIKRSKNG-IRVTQNVFINQLIS*KHNPVT 285 TN + +T + ++ G +++ Q +FI+ LIS H P T Sbjct: 358 TNSLLVMTTLLQAMPGSLKLQQELFISYLISCLHIPST 395 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,237,377 Number of Sequences: 5004 Number of extensions: 39826 Number of successful extensions: 78 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 78 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 78 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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