Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--1041
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58980.1 68418.m07389 ceramidase family protein contains Pfam... 64 1e-10
At1g07380.1 68414.m00787 ceramidase family protein contains simi... 64 1e-10
At2g38010.2 68415.m04666 ceramidase family protein contains Pfam... 62 4e-10
At2g38010.1 68415.m04665 ceramidase family protein contains Pfam... 62 4e-10
At5g17780.1 68418.m02085 hydrolase, alpha/beta fold family prote... 33 0.22
At2g37800.1 68415.m04641 DC1 domain-containing protein contains ... 29 2.0
At5g39865.1 68418.m04835 glutaredoxin family protein 27 8.2
>At5g58980.1 68418.m07389 ceramidase family protein contains Pfam
domain, PF04734: Neutral/alkaline nonlysosomal
ceramidase
Length = 733
Score = 63.7 bits (148), Expect = 1e-10
Identities = 38/125 (30%), Positives = 62/125 (49%)
Frame = +3
Query: 279 LACVTAADALRVGAGIADVTGPPAEIAFMGYAQLEQIGHGIHLRQFSRAFVIEDNSGDTV 458
L C+ + +G G D+TGP A++ MGYA +EQ+ G+H R +RAF++ +
Sbjct: 20 LTCIFSDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFIVAE---PYK 76
Query: 459 KRLVFVSVDAAMMGHGVGKR**DGCRSALA*STTRIM*SSAAPTLTPLPGGFLMDFLFDL 638
KR+ FV++DA M V + + + T + + PGG+L L+ +
Sbjct: 77 KRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYLQYILYLV 136
Query: 639 PILGF 653
LGF
Sbjct: 137 TSLGF 141
>At1g07380.1 68414.m00787 ceramidase family protein contains
similarity to mitochondrial ceramidase [Homo sapiens]
gi|9246993|gb|AAF86240
Length = 779
Score = 63.7 bits (148), Expect = 1e-10
Identities = 28/61 (45%), Positives = 41/61 (67%)
Frame = +3
Query: 312 VGAGIADVTGPPAEIAFMGYAQLEQIGHGIHLRQFSRAFVIEDNSGDTVKRLVFVSVDAA 491
+G G D+TGP A++ MGYA +EQ+ GIH R +R F++ + G KR+VFV++DA
Sbjct: 39 IGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSEPQG---KRVVFVNLDAC 95
Query: 492 M 494
M
Sbjct: 96 M 96
Score = 37.9 bits (84), Expect = 0.006
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = +2
Query: 515 EVIRRLQKRFGVIYNEDNVIISGTHTHS 598
+VI RL+ R+G +Y E NV ISG HTH+
Sbjct: 104 KVIERLKARYGDLYTEQNVGISGIHTHA 131
>At2g38010.2 68415.m04666 ceramidase family protein contains Pfam
domain, PF04734: Neutral/alkaline nonlysosomal
ceramidase
Length = 792
Score = 61.7 bits (143), Expect = 4e-10
Identities = 29/64 (45%), Positives = 42/64 (65%)
Frame = +3
Query: 303 ALRVGAGIADVTGPPAEIAFMGYAQLEQIGHGIHLRQFSRAFVIEDNSGDTVKRLVFVSV 482
A +G G D+TGP A++ MGYA +QI GIH R +RAF++ + G+ R+VFV++
Sbjct: 25 AYLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEPQGN---RVVFVNL 81
Query: 483 DAAM 494
DA M
Sbjct: 82 DACM 85
Score = 38.3 bits (85), Expect = 0.004
Identities = 15/28 (53%), Positives = 21/28 (75%)
Frame = +2
Query: 515 EVIRRLQKRFGVIYNEDNVIISGTHTHS 598
+V+ RL+ R+G +Y E NV ISG HTH+
Sbjct: 93 KVLERLKARYGELYTEKNVAISGIHTHA 120
>At2g38010.1 68415.m04665 ceramidase family protein contains Pfam
domain, PF04734: Neutral/alkaline nonlysosomal
ceramidase
Length = 757
Score = 61.7 bits (143), Expect = 4e-10
Identities = 29/64 (45%), Positives = 42/64 (65%)
Frame = +3
Query: 303 ALRVGAGIADVTGPPAEIAFMGYAQLEQIGHGIHLRQFSRAFVIEDNSGDTVKRLVFVSV 482
A +G G D+TGP A++ MGYA +QI GIH R +RAF++ + G+ R+VFV++
Sbjct: 25 AYLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEPQGN---RVVFVNL 81
Query: 483 DAAM 494
DA M
Sbjct: 82 DACM 85
Score = 38.3 bits (85), Expect = 0.004
Identities = 15/28 (53%), Positives = 21/28 (75%)
Frame = +2
Query: 515 EVIRRLQKRFGVIYNEDNVIISGTHTHS 598
+V+ RL+ R+G +Y E NV ISG HTH+
Sbjct: 93 KVLERLKARYGELYTEKNVAISGIHTHA 120
>At5g17780.1 68418.m02085 hydrolase, alpha/beta fold family protein
low similarity to SP|Q02104 Lipase 1 precursor (EC
3.1.1.3) (Triacylglycerol lipase) {Psychrobacter
immobilis}, SP|P27747|ACOC_ALCEU Dihydrolipoamide
acetyltransferase component of acetoin cleaving system
(EC 2.3.1.12) [Ralstonia eutropha] {Alcaligenes
eutrophus}; contains Pfam profile PF00561: hydrolase,
alpha/beta fold family
Length = 417
Score = 32.7 bits (71), Expect = 0.22
Identities = 10/18 (55%), Positives = 15/18 (83%)
Frame = -2
Query: 632 EEEIHQESSRKWSECGCR 579
+ +IHQES +WS+CGC+
Sbjct: 99 KSKIHQESVNRWSDCGCK 116
>At2g37800.1 68415.m04641 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 396
Score = 29.5 bits (63), Expect = 2.0
Identities = 14/23 (60%), Positives = 15/23 (65%), Gaps = 1/23 (4%)
Frame = +1
Query: 574 HQRH-PHSLHFLEDS*WISSSIC 639
H RH PH H LE S W +SSIC
Sbjct: 255 HVRHVPHPAHLLELSQWGASSIC 277
>At5g39865.1 68418.m04835 glutaredoxin family protein
Length = 390
Score = 27.5 bits (58), Expect = 8.2
Identities = 15/46 (32%), Positives = 24/46 (52%)
Frame = -2
Query: 587 GCR**SHYPRCRLRQSASAAVLLPLSYSMSHHRSINRHEDQSFNSV 450
GC + RCR + + V++P SYSM H +H S+++V
Sbjct: 2 GCASSKNRNRCRNCKGGLSPVIVPRSYSMHVHHPA-QHTGDSYHTV 46
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,638,799
Number of Sequences: 28952
Number of extensions: 307448
Number of successful extensions: 868
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 839
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 866
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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