BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--1001 (628 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g25410.1 68415.m03043 hypothetical protein 29 3.3 At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 28 4.4 At1g63500.1 68414.m07180 protein kinase-related low similarity t... 27 7.7 >At2g25410.1 68415.m03043 hypothetical protein Length = 377 Score = 28.7 bits (61), Expect = 3.3 Identities = 21/76 (27%), Positives = 33/76 (43%), Gaps = 1/76 (1%) Frame = -3 Query: 530 VISLPNLFGAWPSWCAIHYAYYWFYICFAVHNEF-DGVNNQLWHFRYDDSKSFDCDRRQA 354 V+ NL G+ PS C I + F + + DG N++ ++D DC+RR Sbjct: 153 VVQFENL-GSMPSSCHIFKILPLPFSWFVAYTTYPDGQNSRDMWLKWDSPDCRDCERRTN 211 Query: 353 CCVSFAHDNSRDHVNC 306 F +N+ V C Sbjct: 212 SRCGF-KNNTSHQVEC 226 >At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein similar to SP|Q13144 Translation initiation factor eIF-2B epsilon subunit (eIF-2B GDP-GTP exchange factor) {Homo sapiens}; contains Pfam profile PF02020: eIF4-gamma/eIF5/eIF2-epsilon Length = 676 Score = 28.3 bits (60), Expect = 4.4 Identities = 11/35 (31%), Positives = 23/35 (65%) Frame = +3 Query: 318 VSAIIMGKAYATSLPPITVEALGIIIPKVPQLIVD 422 + AI++ ++AT L P+T+E +++P V ++D Sbjct: 25 LQAILLADSFATKLLPLTLERPNVLLPLVNIPMID 59 >At1g63500.1 68414.m07180 protein kinase-related low similarity to protein kinase [Arabidopsis thaliana]; contains Pfam profile: PF00069 Eukaryotic protein kinase domain Length = 422 Score = 27.5 bits (58), Expect = 7.7 Identities = 15/66 (22%), Positives = 33/66 (50%) Frame = -3 Query: 413 QLWHFRYDDSKSFDCDRRQACCVSFAHDNSRDHVNCLMKYIQRTVHVFQSMFNFNQVTSV 234 Q+W + DS +F ++ V+F H + ++C ++I+ V +++ + + Sbjct: 308 QMWTNQMQDSLNF----KKKGDVAFRHKEFANAIDCYSQFIEGGTMVSPTVYARRSLCYL 363 Query: 233 MSNMPQ 216 M+ MPQ Sbjct: 364 MNEMPQ 369 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,595,046 Number of Sequences: 28952 Number of extensions: 240989 Number of successful extensions: 684 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 669 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 684 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1275599520 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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