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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ce--0987
         (359 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g17280.1 68414.m02105 ubiquitin-conjugating enzyme, putative ...    29   0.70 
At2g42700.1 68415.m05287 expressed protein                             27   5.0  
At5g44000.1 68418.m05384 glutathione S-transferase C-terminal do...    26   6.6  
At1g08650.1 68414.m00960 phosphoenolpyruvate carboxylase kinase ...    26   8.7  

>At1g17280.1 68414.m02105 ubiquitin-conjugating enzyme, putative
           similar to ubiquitin conjugating enzyme 6 from [Homo
           sapiens] GI:14029267, [Mus musculus] GI:14029263;
           contains Pfam profile PF00179: Ubiquitin-conjugating
           enzyme
          Length = 237

 Score = 29.5 bits (63), Expect = 0.70
 Identities = 17/68 (25%), Positives = 32/68 (47%)
 Frame = +2

Query: 47  KAFPVSPGQVGEQRLSQEGWDLLTTARAPPKET*QLKSNCFANESTTGSESRPAEKIRLE 226
           K FP    +  +Q+L+++    LTT  +P K   +++S    + +   SE    E+ +  
Sbjct: 151 KLFPEYVEKYNQQQLAEQATTQLTTPESPQKSDTKVESEKTIDPTKGDSEGGLKERKKNN 210

Query: 227 TQRADAWV 250
            Q   AW+
Sbjct: 211 KQGLPAWI 218


>At2g42700.1 68415.m05287 expressed protein 
          Length = 788

 Score = 26.6 bits (56), Expect = 5.0
 Identities = 12/36 (33%), Positives = 17/36 (47%)
 Frame = +1

Query: 43  PEGLSSFTRTGGRAKAQPRGVGFANNCPSASEGDLT 150
           PEG+ +F R      + P+ VG  N C   S  + T
Sbjct: 325 PEGIEAFLRGWDSYTSAPQNVGLFNECDKKSTTNWT 360


>At5g44000.1 68418.m05384 glutathione S-transferase C-terminal
           domain-containing protein contains Pfam domain PF00043:
           Glutathione S-transferase, C-terminal domain
          Length = 399

 Score = 26.2 bits (55), Expect = 6.6
 Identities = 14/39 (35%), Positives = 20/39 (51%)
 Frame = +2

Query: 110 LLTTARAPPKET*QLKSNCFANESTTGSESRPAEKIRLE 226
           L++TAR       QL     A   ++GS +RP  K RL+
Sbjct: 56  LISTARTAWTTVWQLMMTQLAPSDSSGSYTRPTSKFRLD 94


>At1g08650.1 68414.m00960 phosphoenolpyruvate carboxylase kinase
           identical to phosphoenolpyruvate carboxylase kinase
           [Arabidopsis thaliana] gi|6318613|gb|AAF06968; contains
           protein kinase domain, Pfam:PF00069
          Length = 284

 Score = 25.8 bits (54), Expect = 8.7
 Identities = 15/45 (33%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
 Frame = -3

Query: 285 LVELDKEVDV--HLTHASAR*VSSRIFSAGRDSDPVVDSFAKQLL 157
           L++ D  + +   L H S   +  R+ S+G   +P   SFAKQ+L
Sbjct: 82  LIDTDSTLSIFMELVHPSVS-IYDRLVSSGTFFEPQTASFAKQIL 125


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,602,600
Number of Sequences: 28952
Number of extensions: 113826
Number of successful extensions: 253
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 252
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 253
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 469342752
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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