BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0965 (555 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g10930.1 68414.m01255 DNA helicase (RECQl4A) nearly identical... 32 0.22 At4g38560.1 68417.m05459 expressed protein 29 2.8 At1g36990.1 68414.m04611 expressed protein contains PS00070: Ald... 29 2.8 At5g38830.1 68418.m04697 tRNA synthetase class I (C) family prot... 28 4.8 At2g29910.2 68415.m03633 F-box family protein contains F-box dom... 28 4.8 At2g29910.1 68415.m03632 F-box family protein contains F-box dom... 28 4.8 At5g27610.1 68418.m03308 myb family transcription factor contain... 27 6.4 At2g25900.1 68415.m03108 zinc finger (CCCH-type) family protein ... 27 6.4 At5g64050.1 68418.m08042 glutamate-tRNA ligase family protein / ... 27 8.4 At4g36280.1 68417.m05159 ATP-binding region, ATPase-like domain-... 27 8.4 >At1g10930.1 68414.m01255 DNA helicase (RECQl4A) nearly identical to DNA Helicase [Arabidopsis thaliana] GI:11121449 Length = 1188 Score = 32.3 bits (70), Expect = 0.22 Identities = 15/38 (39%), Positives = 24/38 (63%), Gaps = 2/38 (5%) Frame = +3 Query: 387 DQAKFACDWWYNV-DCSQAIEHYKLNADPL-KNPYVPK 494 DQ ++ACD W D S +++ Y L++ P+ + YVPK Sbjct: 378 DQGRYACDSWNTPRDSSFSVDRYGLSSAPVEREQYVPK 415 >At4g38560.1 68417.m05459 expressed protein Length = 521 Score = 28.7 bits (61), Expect = 2.8 Identities = 12/36 (33%), Positives = 19/36 (52%) Frame = +3 Query: 432 SQAIEHYKLNADPLKNPYVPKQKPEITRNNPEEFNI 539 +Q + H L + LK+P + +P I + P FNI Sbjct: 196 TQPVPHRSLESAGLKSPQKGETRPHIPQTQPTSFNI 231 >At1g36990.1 68414.m04611 expressed protein contains PS00070: Aldehyde dehydrogenases cysteine active site; similar to high molecular mass nuclear antigen (GI:2754696) [Gallus gallus];similar to streptococcal hemagglutinin (GI:8885520) [Streptococcus gordonii] similar to proteophosphoglycan (GI:5420389) [Leishmania major] Length = 581 Score = 28.7 bits (61), Expect = 2.8 Identities = 15/51 (29%), Positives = 24/51 (47%) Frame = +1 Query: 28 EMGKAVLRALTGALLCGVVSDAAYLQRYEPYGLQFEQALIRKPLREHEKPQ 180 E+ + +A TG+L + + P GL +AL++ P R H PQ Sbjct: 236 EVPNVIEKACTGSLTSPKANAVSAGTLTGPSGLNMAEALVQAPARTHTPPQ 286 >At5g38830.1 68418.m04697 tRNA synthetase class I (C) family protein similar to SP|Q06752 Cysteinyl-tRNA synthetase (EC 6.1.1.16) (Cysteine--tRNA ligase) (CysRS) {Bacillus subtilis}; contains Pfam profile PF01406: tRNA synthetases class I (C) Length = 511 Score = 27.9 bits (59), Expect = 4.8 Identities = 10/23 (43%), Positives = 11/23 (47%) Frame = -3 Query: 391 WSNRVPLVQRNEAPW*PSRPSWH 323 W P E+PW P RP WH Sbjct: 189 WKAAKPDEPSWESPWGPGRPGWH 211 >At2g29910.2 68415.m03633 F-box family protein contains F-box domain Pfam:PF00646 Length = 335 Score = 27.9 bits (59), Expect = 4.8 Identities = 13/37 (35%), Positives = 16/37 (43%) Frame = +3 Query: 336 REGHQGASFLCTNGTLFDQAKFACDWWYNVDCSQAIE 446 RE H G F N D + + D WY VD +E Sbjct: 216 REAHDGCWFRTPNLVYLDYSSYVPD-WYEVDLGSLVE 251 >At2g29910.1 68415.m03632 F-box family protein contains F-box domain Pfam:PF00646 Length = 352 Score = 27.9 bits (59), Expect = 4.8 Identities = 13/37 (35%), Positives = 16/37 (43%) Frame = +3 Query: 336 REGHQGASFLCTNGTLFDQAKFACDWWYNVDCSQAIE 446 RE H G F N D + + D WY VD +E Sbjct: 216 REAHDGCWFRTPNLVYLDYSSYVPD-WYEVDLGSLVE 251 >At5g27610.1 68418.m03308 myb family transcription factor contains Pfam profile: PF00249 myb-like DNA-binding domain Length = 969 Score = 27.5 bits (58), Expect = 6.4 Identities = 11/30 (36%), Positives = 19/30 (63%) Frame = -1 Query: 297 QRSRTFQGVSARAHRESSLRNGVVDGVVHA 208 ++S T + VS HRE + + G+ D ++HA Sbjct: 347 KKSNTLETVSTSHHREKAKQAGLEDNLLHA 376 >At2g25900.1 68415.m03108 zinc finger (CCCH-type) family protein contains Pfam domain, PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and similar) Length = 315 Score = 27.5 bits (58), Expect = 6.4 Identities = 11/29 (37%), Positives = 14/29 (48%) Frame = -1 Query: 522 GCSW*FPVSVSARRDFSMDQRSVCNVQWP 436 GCSW P+ + R FS R + WP Sbjct: 250 GCSWSSPMRSAVRLPFSSSLRPIQAATWP 278 >At5g64050.1 68418.m08042 glutamate-tRNA ligase family protein / glutamyl-tRNA synthetase family protein similar to SP|P22250 from Bacillus subtilis, SP|P22249 from Bacillus stearothermophilus; contains Pfam tRNA synthetases class I (E and Q), catalytic domain PF00749 Length = 570 Score = 27.1 bits (57), Expect = 8.4 Identities = 19/41 (46%), Positives = 21/41 (51%), Gaps = 3/41 (7%) Frame = -2 Query: 245 VSGTEW*MG*STPGV-PGTLRR--SCGFSCSLRGFLIKACS 132 V GT W S P + P LRR S F CS R F + ACS Sbjct: 5 VYGTPWLRVRSLPELAPAFLRRRQSSLFYCSRRSFAVVACS 45 >At4g36280.1 68417.m05159 ATP-binding region, ATPase-like domain-containing protein low similarity to microrchidia [Mus musculus] GI:5410255; contains Pfam profile PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein Length = 626 Score = 27.1 bits (57), Expect = 8.4 Identities = 13/43 (30%), Positives = 19/43 (44%) Frame = +3 Query: 396 KFACDWWYNVDCSQAIEHYKLNADPLKNPYVPKQKPEITRNNP 524 K +WY+ Y++ AD K +P Q P I+ NP Sbjct: 456 KIVYSYWYSHCHLLGYHKYQMPADKSKKIAIPDQPPTISTVNP 498 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,118,262 Number of Sequences: 28952 Number of extensions: 248337 Number of successful extensions: 732 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 717 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 732 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1053014392 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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