Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--0854
(749 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 148 1e-36
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 141 1e-34
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 75 1e-14
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 59 8e-10
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 29 0.54
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 29 0.94
SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.8
SPAC11E3.11c |||guanyl-nucleotide exchange factor |Schizosacchar... 26 5.0
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 26 5.0
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.6
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 8.7
SPCC1322.06 |kap113||karyopherin Kap113|Schizosaccharomyces pomb... 25 8.7
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 148 bits (358), Expect = 1e-36
Identities = 64/82 (78%), Positives = 76/82 (92%)
Frame = +1
Query: 502 LIVERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYD 681
L++ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA YD
Sbjct: 156 LLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYD 215
Query: 682 ICRRNLDIERPTYTNLNRLIGQ 747
ICRRNLDIERPTY NLNRLI Q
Sbjct: 216 ICRRNLDIERPTYENLNRLIAQ 237
Score = 128 bits (308), Expect = 1e-30
Identities = 51/70 (72%), Positives = 65/70 (92%)
Frame = +2
Query: 257 LEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQC 436
LEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C
Sbjct: 74 LEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNC 133
Query: 437 TGLQGFLIFH 466
+GLQGFL+FH
Sbjct: 134 SGLQGFLVFH 143
Score = 101 bits (242), Expect = 1e-22
Identities = 50/73 (68%), Positives = 55/73 (75%), Gaps = 5/73 (6%)
Frame = +3
Query: 51 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 215
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 216 TGAGKHVPRAVFV 254
TG GK VPR+++V
Sbjct: 60 TGQGKFVPRSIYV 72
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 141 bits (341), Expect = 1e-34
Identities = 61/82 (74%), Positives = 75/82 (91%)
Frame = +1
Query: 502 LIVERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYD 681
L++ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YD
Sbjct: 152 LLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYD 211
Query: 682 ICRRNLDIERPTYTNLNRLIGQ 747
ICRRNLDIERP+Y NLNRLI Q
Sbjct: 212 ICRRNLDIERPSYENLNRLIAQ 233
Score = 129 bits (311), Expect = 5e-31
Identities = 54/70 (77%), Positives = 64/70 (91%)
Frame = +2
Query: 257 LEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQC 436
LEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C
Sbjct: 70 LEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNC 129
Query: 437 TGLQGFLIFH 466
+GLQGFL+FH
Sbjct: 130 SGLQGFLVFH 139
Score = 108 bits (260), Expect = 7e-25
Identities = 46/68 (67%), Positives = 54/68 (79%)
Frame = +3
Query: 51 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 230
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 231 HVPRAVFV 254
+VPR+++V
Sbjct: 61 YVPRSIYV 68
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 74.5 bits (175), Expect = 1e-14
Identities = 32/70 (45%), Positives = 46/70 (65%)
Frame = +2
Query: 257 LEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQC 436
LEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C
Sbjct: 68 LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127
Query: 437 TGLQGFLIFH 466
LQGF + H
Sbjct: 128 DALQGFQLTH 137
Score = 70.5 bits (165), Expect = 2e-13
Identities = 31/80 (38%), Positives = 50/80 (62%)
Frame = +1
Query: 502 LIVERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYD 681
L++ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DNEA+
Sbjct: 150 LLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSS 209
Query: 682 ICRRNLDIERPTYTNLNRLI 741
I L I+ P+Y +LN L+
Sbjct: 210 IFANTLKIKSPSYDDLNHLV 229
Score = 51.2 bits (117), Expect = 2e-07
Identities = 26/68 (38%), Positives = 34/68 (50%)
Frame = +3
Query: 51 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 230
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 231 HVPRAVFV 254
+VPRAV V
Sbjct: 59 YVPRAVLV 66
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 58.8 bits (136), Expect = 8e-10
Identities = 33/96 (34%), Positives = 54/96 (56%), Gaps = 1/96 (1%)
Frame = +3
Query: 54 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 233
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 234 VPRAVFVIXXXXXXMRSALAHT-DSCFIQNNLLLVR 338
+PRA+ +I + + L+ T S + N+L+ +
Sbjct: 61 IPRAI-LIDLEPRVVNNILSDTYGSLYNPENILITK 95
Score = 52.0 bits (119), Expect = 9e-08
Identities = 27/80 (33%), Positives = 45/80 (56%), Gaps = 1/80 (1%)
Frame = +1
Query: 505 IVERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYD 681
++ERL+ Y KK ++++P Q VS VV+PYNS+L ++D ++DN A+
Sbjct: 153 LLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAH 212
Query: 682 ICRRNLDIERPTYTNLNRLI 741
I L + PT+ N+L+
Sbjct: 213 IAADRLHTQNPTFHQQNQLV 232
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 29.5 bits (63), Expect = 0.54
Identities = 24/73 (32%), Positives = 37/73 (50%)
Frame = +1
Query: 40 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCPQTRPSGVETILSTLSSARP 219
+K S + +++ P S SV P S T S++SS A PQ+ S ++S +SS
Sbjct: 456 VKQPTASVASSSVSVPSSSSVQPQSS-TPISSSSS--ASSPQSTLSTSSEVVSEVSSTLL 512
Query: 220 ELASTYPVLSSSS 258
+S P SSS+
Sbjct: 513 SGSSAIPSTSSST 525
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 28.7 bits (61), Expect = 0.94
Identities = 20/43 (46%), Positives = 24/43 (55%)
Frame = +1
Query: 130 STASSLMARCPQTRPSGVETILSTLSSARPELASTYPVLSSSS 258
ST SSL + ++PS T ST SSA P S+ LSSSS
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS-SYLSSSS 214
>SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 317
Score = 26.6 bits (56), Expect = 3.8
Identities = 25/74 (33%), Positives = 34/74 (45%)
Frame = +1
Query: 37 KLKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCPQTRPSGVETILSTLSSAR 216
K SK S+S T P S S A A TAS+ + + S V +++S+ SSA
Sbjct: 216 KSSSKFSSSSFITSTTPASSSSSGAIVSNA-KTASTDDSSSASSATSSVSSVVSSASSAL 274
Query: 217 PELASTYPVLSSSS 258
AS+ SSS
Sbjct: 275 SASASSASASVSSS 288
>SPAC11E3.11c |||guanyl-nucleotide exchange factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 942
Score = 26.2 bits (55), Expect = 5.0
Identities = 30/99 (30%), Positives = 50/99 (50%), Gaps = 6/99 (6%)
Frame = +1
Query: 37 KLKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARC----PQTRPSGVETILSTL 204
K KSK ++ + P S+SVM S T+ T +S +R R G+ T +S++
Sbjct: 85 KDKSKASMSTNDLPSHPRSQSVMGFSSSTSQLTGTSNSSRTRLNKDMRRDFGM-TSMSSI 143
Query: 205 SSARPELASTYPVLSSSS*TYC--C**GPHWHIQTVVSS 315
+S+ P S PV S+S ++ GP + +T++SS
Sbjct: 144 TSSTP-TPSQLPVRPSTSLSFFDDIPLGPSFSAETILSS 181
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 26.2 bits (55), Expect = 5.0
Identities = 23/74 (31%), Positives = 37/74 (50%), Gaps = 3/74 (4%)
Frame = +1
Query: 46 SKCVSASLYTLAKPESRSVMPAGSFTAWS---TASSLMARCPQTRPSGVETILSTLSSAR 216
S S+S + + S S +P S T+ S ++SS + +RPS + ++T+SS+
Sbjct: 189 SSSSSSSSSSSSSSSSSSSVPITSSTSSSHSSSSSSSSSSSSSSRPSSSSSFITTMSSS- 247
Query: 217 PELASTYPVLSSSS 258
ST V SSS
Sbjct: 248 -TFISTVTVTPSSS 260
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.6
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 149 WPDAHRQDHRGWRRFFQHFLQR 214
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 8.7
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +1
Query: 40 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 162
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPCC1322.06 |kap113||karyopherin Kap113|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 983
Score = 25.4 bits (53), Expect = 8.7
Identities = 24/83 (28%), Positives = 36/83 (43%), Gaps = 6/83 (7%)
Frame = +1
Query: 22 FKHRKKLKSKCVSASLYTLAK----PESRS--VMPAGSFTAWSTASSLMARCPQTRPSGV 183
F + K KS S LYT+ + PES + A + WST + + P+T +
Sbjct: 648 FVNAIKAKSFEFSTFLYTVIEYCVNPESPEHVIFEADAMELWSTFLMYIQKLPETFTLLI 707
Query: 184 ETILSTLSSARPELASTYPVLSS 252
+L LS A L ++SS
Sbjct: 708 PHLLYHLSQATSTLPFVLMIVSS 730
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,139,226
Number of Sequences: 5004
Number of extensions: 65945
Number of successful extensions: 222
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 199
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 217
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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