Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ce--0791
         (455 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   137   9e-34
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   125   3e-30
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    74   9e-15
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    69   3e-13
SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch...    27   1.8  
SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ...    26   3.1  
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   3.1  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   3.1  
SPBC16E9.08 |mcp4|mug101|sequence orphan|Schizosaccharomyces pom...    25   5.5  
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo...    25   7.3  
SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c...    24   9.6  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  137 bits (331), Expect = 9e-34
 Identities = 59/88 (67%), Positives = 72/88 (81%)
 Frame = +3

Query: 3   LEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQ 182
           LEHGIQP+G M  +      D  F+TFFSETG GK+VPR+++VDLEP V+D+VRTG YR 
Sbjct: 26  LEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRD 85

Query: 183 LFHPEQLITGKEDAANNYARGHYTLERK 266
           LFHPEQLITGKEDA+NNYARGHYT+ ++
Sbjct: 86  LFHPEQLITGKEDASNNYARGHYTVGKE 113



 Score = 51.6 bits (118), Expect = 6e-08
 Identities = 20/30 (66%), Positives = 27/30 (90%)
 Frame = +2

Query: 254 LGKEIVDLVLDRIRKLADQCTGLQGFLIFH 343
           +GKE+VD V D+IR++AD C+GLQGFL+FH
Sbjct: 110 VGKELVDEVTDKIRRIADNCSGLQGFLVFH 139



 Score = 33.5 bits (73), Expect = 0.016
 Identities = 12/19 (63%), Positives = 18/19 (94%)
 Frame = +1

Query: 397 LSVDYGKKSKLEFAIYPAP 453
           L+++Y KKSKL+F++YPAP
Sbjct: 157 LAMEYTKKSKLQFSVYPAP 175


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  125 bits (302), Expect = 3e-30
 Identities = 58/93 (62%), Positives = 72/93 (77%), Gaps = 5/93 (5%)
 Frame = +3

Query: 3   LEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRT 167
           LEHGI PDG  PT+     K     +D F TFFSETG GK VPR+++VDLEP V+D+VRT
Sbjct: 26  LEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVRT 84

Query: 168 GTYRQLFHPEQLITGKEDAANNYARGHYTLERK 266
           G Y+ LFHPEQ++TGKEDA+NNYARGHYT+ ++
Sbjct: 85  GPYKDLFHPEQMVTGKEDASNNYARGHYTVGKE 117



 Score = 52.8 bits (121), Expect = 2e-08
 Identities = 20/30 (66%), Positives = 28/30 (93%)
 Frame = +2

Query: 254 LGKEIVDLVLDRIRKLADQCTGLQGFLIFH 343
           +GKE++D VL+RIR++AD C+GLQGFL+FH
Sbjct: 114 VGKEMIDSVLERIRRMADNCSGLQGFLVFH 143



 Score = 34.7 bits (76), Expect = 0.007
 Identities = 12/19 (63%), Positives = 18/19 (94%)
 Frame = +1

Query: 397 LSVDYGKKSKLEFAIYPAP 453
           L+++YGKKS L+F++YPAP
Sbjct: 161 LNMEYGKKSNLQFSVYPAP 179


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 74.1 bits (174), Expect = 9e-15
 Identities = 35/83 (42%), Positives = 49/83 (59%)
 Frame = +3

Query: 6   EHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQL 185
           EHG+   G      T     +  N +F+E   GK+VPRAV VDLEP  +D V++G +  L
Sbjct: 27  EHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNL 84

Query: 186 FHPEQLITGKEDAANNYARGHYT 254
           F P+ +I G+  A N +A+GHYT
Sbjct: 85  FRPDNIIYGQSGAGNIWAKGHYT 107



 Score = 34.3 bits (75), Expect = 0.009
 Identities = 14/29 (48%), Positives = 19/29 (65%)
 Frame = +2

Query: 257 GKEIVDLVLDRIRKLADQCTGLQGFLIFH 343
           G E+ D VLD +R+ A+ C  LQGF + H
Sbjct: 109 GAELADAVLDVVRREAEACDALQGFQLTH 137


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 69.3 bits (162), Expect = 3e-13
 Identities = 35/89 (39%), Positives = 54/89 (60%), Gaps = 2/89 (2%)
 Frame = +3

Query: 3   LEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQ 182
           LEHGI PDG + +  T   G D  + FF ++   +++PRA+ +DLEP VV+ + + TY  
Sbjct: 27  LEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGS 84

Query: 183 LFHPEQLITGKE--DAANNYARGHYTLER 263
           L++PE ++  K    A NN+A G+   ER
Sbjct: 85  LYNPENILITKNGGGAGNNWANGYSHAER 113


>SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 425

 Score = 26.6 bits (56), Expect = 1.8
 Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
 Frame = -3

Query: 324 CRPVHWSASLRIL--SKTKSTISFPRCSD 244
           C   H+S +  +L  SKT S IS P C+D
Sbjct: 229 CPSCHYSTNSEMLDLSKTSSDISCPNCND 257


>SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1098

 Score = 25.8 bits (54), Expect = 3.1
 Identities = 14/35 (40%), Positives = 15/35 (42%)
 Frame = +3

Query: 156  EVRTGTYRQLFHPEQLITGKEDAANNYARGHYTLE 260
            EVR       F  E LI GK+     Y R H  LE
Sbjct: 1010 EVRGSQMIDTFSTELLIPGKQRVQRTYRRTHEVLE 1044


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 3.1
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +1

Query: 7   STASSLMARCPQTRPSGVETILSTLSSARPELAST 111
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 3.1
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +2

Query: 26  WPDAHRQDHRGWRRFFQHFLQR 91
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


>SPBC16E9.08 |mcp4|mug101|sequence orphan|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 355

 Score = 25.0 bits (52), Expect = 5.5
 Identities = 17/44 (38%), Positives = 20/44 (45%), Gaps = 1/44 (2%)
 Frame = -3

Query: 339 KIRNPCRPVH-WSASLRILSKTKSTISFPRCSDHGHSCWPHLPY 211
           KIRN    V  +  +L ILSK  S  SF R   +   CW    Y
Sbjct: 94  KIRNSVPNVSAFRNNLSILSKELSFFSFARHIHNRRLCWAEFIY 137


>SPBC4.03c |||COPII-coated vesicle component Sfb3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 891

 Score = 24.6 bits (51), Expect = 7.3
 Identities = 12/38 (31%), Positives = 18/38 (47%), Gaps = 1/38 (2%)
 Frame = -2

Query: 418 SCRSQRRDRSINKKG-EPRAGTSTEGVEDQESLQTGTL 308
           +C     + S N  G  P  G+  +GVE +  L  GT+
Sbjct: 237 TCNLCGSENSFNDDGFNPLVGSQHDGVESRPELNVGTI 274


>SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 649

 Score = 24.2 bits (50), Expect = 9.6
 Identities = 8/19 (42%), Positives = 14/19 (73%)
 Frame = +3

Query: 213 KEDAANNYARGHYTLERKS 269
           +E  ANN+ RGH  +++K+
Sbjct: 599 RERKANNFFRGHIEIDKKT 617


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,945,486
Number of Sequences: 5004
Number of extensions: 37523
Number of successful extensions: 158
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 170285640
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)