BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0666 (566 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g25100.1 68418.m02974 endomembrane protein 70, putative TM4 f... 28 5.0 At5g01440.1 68418.m00057 insulin-degrading enzyme-related contai... 27 6.6 >At5g25100.1 68418.m02974 endomembrane protein 70, putative TM4 family; Length = 644 Score = 27.9 bits (59), Expect = 5.0 Identities = 11/30 (36%), Positives = 21/30 (70%) Frame = -1 Query: 281 IYLVIILLIST*IPVTIIIHYVRLFHEDWL 192 ++LV ++L+ T +TI++ Y +L ED+L Sbjct: 539 LFLVFVILMVTCAEITIVLCYFQLCSEDYL 568 >At5g01440.1 68418.m00057 insulin-degrading enzyme-related contains weak similarity to Swiss-Prot:P14735 insulin-degrading enzyme (Insulysin) (Insulinase) (Insulin protease) [Homo sapiens] Length = 307 Score = 27.5 bits (58), Expect = 6.6 Identities = 14/32 (43%), Positives = 23/32 (71%) Frame = +1 Query: 331 SCDFNFFTIFYKLVSDIIFQNDINEFHKRYNR 426 SC F+F++ K +S +I +ND+ E++KRY R Sbjct: 149 SCIFDFYSEEKKELS-LITKNDLIEWYKRYVR 179 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,322,956 Number of Sequences: 28952 Number of extensions: 181852 Number of successful extensions: 347 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 346 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 347 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1092379416 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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