Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--0662
(612 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g31180.2 68417.m04427 aspartyl-tRNA synthetase, putative / as... 133 1e-31
At4g31180.1 68417.m04426 aspartyl-tRNA synthetase, putative / as... 133 1e-31
At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative / as... 129 2e-30
At4g17300.1 68417.m02598 asparaginyl-tRNA synthetase, chloroplas... 48 4e-06
At5g56680.1 68418.m07075 asparaginyl-tRNA synthetase 1, cytoplas... 44 1e-04
At1g70980.1 68414.m08188 asparaginyl-tRNA synthetase, cytoplasmi... 42 3e-04
At4g33760.1 68417.m04793 tRNA synthetase class II (D, K and N) f... 42 4e-04
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f... 34 0.065
At3g07420.1 68416.m00884 asparaginyl-tRNA synthetase 2, cytoplas... 30 1.4
At5g52070.1 68418.m06464 agenet domain-containing protein contai... 29 1.8
At1g70640.1 68414.m08143 octicosapeptide/Phox/Bem1p (PB1) domain... 29 2.4
At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative / lysin... 29 3.2
At1g62020.1 68414.m06995 coatomer protein complex, subunit alpha... 29 3.2
At3g19010.1 68416.m02413 oxidoreductase, 2OG-Fe(II) oxygenase fa... 28 4.2
At2g43920.1 68415.m05459 thiol methyltransferase, putative simil... 28 4.2
At2g21390.1 68415.m02546 coatomer protein complex, subunit alpha... 28 4.2
At2g17340.1 68415.m02003 pantothenate kinase-related contains Pf... 27 7.4
At4g28990.1 68417.m04143 RNA-binding protein-related contains we... 27 9.8
>At4g31180.2 68417.m04427 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative similar to
Aspartyl-tRNA synthetase (Aspartate--tRNA ligase)
(AspRS) [Homo sapiens] GI:20178330
Length = 558
Score = 133 bits (321), Expect = 1e-31
Identities = 65/116 (56%), Positives = 81/116 (69%)
Frame = -1
Query: 603 EE*ATVGQQYRVEPFKFLDPPLKAGVSAGRPELKEAGVSVGDEDDLSTPDEKLLGRLVRA 424
+E VG+QY EP KFL L+ G LKEAGV V DL+T E+ LG+LV
Sbjct: 380 KELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVDPLGDLNTESERKLGQLVLE 439
Query: 423 KYDTDFYILDKYPLAVRPFYTMPDPGNPKYSNSYDMFMRGEEILSGAQRIHDPDLL 256
KY+T+FYIL +YP AVRPFYTM NP YSNS+D+F+RGEEI+SGAQR+H P++L
Sbjct: 440 KYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGEEIISGAQRVHIPEVL 495
Score = 83.4 bits (197), Expect = 1e-16
Identities = 35/56 (62%), Positives = 45/56 (80%)
Frame = -3
Query: 235 GVDTSKIAAYIESFRLGCPPHAGGGIGLERVVMLYLGLDNIRKTSMFPRDPKRVTP 68
G+D I+ YI+SFR G P H G G+GLERVVML+ L+NIRKTS+FPRDP+R++P
Sbjct: 503 GIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALNNIRKTSLFPRDPQRLSP 558
>At4g31180.1 68417.m04426 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative similar to
Aspartyl-tRNA synthetase (Aspartate--tRNA ligase)
(AspRS) [Homo sapiens] GI:20178330
Length = 558
Score = 133 bits (321), Expect = 1e-31
Identities = 65/116 (56%), Positives = 81/116 (69%)
Frame = -1
Query: 603 EE*ATVGQQYRVEPFKFLDPPLKAGVSAGRPELKEAGVSVGDEDDLSTPDEKLLGRLVRA 424
+E VG+QY EP KFL L+ G LKEAGV V DL+T E+ LG+LV
Sbjct: 380 KELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVDPLGDLNTESERKLGQLVLE 439
Query: 423 KYDTDFYILDKYPLAVRPFYTMPDPGNPKYSNSYDMFMRGEEILSGAQRIHDPDLL 256
KY+T+FYIL +YP AVRPFYTM NP YSNS+D+F+RGEEI+SGAQR+H P++L
Sbjct: 440 KYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGEEIISGAQRVHIPEVL 495
Score = 83.4 bits (197), Expect = 1e-16
Identities = 35/56 (62%), Positives = 45/56 (80%)
Frame = -3
Query: 235 GVDTSKIAAYIESFRLGCPPHAGGGIGLERVVMLYLGLDNIRKTSMFPRDPKRVTP 68
G+D I+ YI+SFR G P H G G+GLERVVML+ L+NIRKTS+FPRDP+R++P
Sbjct: 503 GIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALNNIRKTSLFPRDPQRLSP 558
>At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative simialr to
aspartate-tRNA ligase (EC 6.1.1.12) from Drosophila
melanogaster GI:4512034, Homo sapiens SP|P14868, Rattus
norvegicus SP|P15178; contains Pfam profile PF00152 tRNA
synthetases class II (D, K and N)
Length = 532
Score = 129 bits (311), Expect = 2e-30
Identities = 63/117 (53%), Positives = 81/117 (69%)
Frame = -1
Query: 606 PEE*ATVGQQYRVEPFKFLDPPLKAGVSAGRPELKEAGVSVGDEDDLSTPDEKLLGRLVR 427
P+E +V +QY + KFL L+ + G LKEAG V DL+T E+ LG+LV
Sbjct: 353 PKELESVRKQYPFQSLKFLPQTLRLTFAEGIQMLKEAGEEVDPLGDLNTESERKLGQLVL 412
Query: 426 AKYDTDFYILDKYPLAVRPFYTMPDPGNPKYSNSYDMFMRGEEILSGAQRIHDPDLL 256
KY T+FY+L +YP AVRPFYTMP + YSNS+D+F+RGEEI+SGAQRIHDP+LL
Sbjct: 413 EKYKTEFYMLHRYPSAVRPFYTMPYENDSNYSNSFDVFIRGEEIMSGAQRIHDPELL 469
Score = 83.0 bits (196), Expect = 1e-16
Identities = 35/56 (62%), Positives = 44/56 (78%)
Frame = -3
Query: 235 GVDTSKIAAYIESFRLGCPPHAGGGIGLERVVMLYLGLDNIRKTSMFPRDPKRVTP 68
G+D I+ YI++FR G PPH G G+GLERVVML L+NIRKTS+FPRD +R+TP
Sbjct: 477 GIDVKTISTYIDAFRYGAPPHGGFGVGLERVVMLLCALNNIRKTSLFPRDSQRLTP 532
>At4g17300.1 68417.m02598 asparaginyl-tRNA synthetase, chloroplast,
mitochondrial / asparagine-tRNA ligase / AsnRS (SYNO)
nearly identical to SP|O48593
Length = 567
Score = 48.4 bits (110), Expect = 4e-06
Identities = 21/42 (50%), Positives = 26/42 (61%)
Frame = -3
Query: 208 YIESFRLGCPPHAGGGIGLERVVMLYLGLDNIRKTSMFPRDP 83
Y++ R G PHAG G+G ER+V G+DNIR FPR P
Sbjct: 521 YLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDVIPFPRTP 562
>At5g56680.1 68418.m07075 asparaginyl-tRNA synthetase 1, cytoplasmic
/ asparagine-tRNA ligase 1 (SYNC1) identical to
SP|Q9SW96
Length = 572
Score = 43.6 bits (98), Expect = 1e-04
Identities = 19/42 (45%), Positives = 25/42 (59%)
Frame = -3
Query: 208 YIESFRLGCPPHAGGGIGLERVVMLYLGLDNIRKTSMFPRDP 83
Y++ R G H G G+G ER+++ GLDNIR FPR P
Sbjct: 526 YLDLRRYGTVKHCGFGLGFERMILFATGLDNIRDVIPFPRYP 567
>At1g70980.1 68414.m08188 asparaginyl-tRNA synthetase, cytoplasmic,
putative / asparagine-tRNA ligase, putative similar to
SYNC1 protein GI:5670315 [SP|Q9SW96] from [Arabidopsis
thaliana]
Length = 571
Score = 41.9 bits (94), Expect = 3e-04
Identities = 18/42 (42%), Positives = 24/42 (57%)
Frame = -3
Query: 208 YIESFRLGCPPHAGGGIGLERVVMLYLGLDNIRKTSMFPRDP 83
Y++ R G H G G+G ER++ G+DNIR FPR P
Sbjct: 525 YLDLRRYGTVKHCGFGLGFERMIQFATGIDNIRDVIPFPRYP 566
>At4g33760.1 68417.m04793 tRNA synthetase class II (D, K and N)
family protein similar to SP|P36419 Aspartyl-tRNA
synthetase (EC 6.1.1.12) (Aspartate--tRNA ligase)
(AspRS) {Thermus thermophilus}; contains Pfam profile
PF00152: tRNA synthetases class II (D, K and N)
Length = 664
Score = 41.5 bits (93), Expect = 4e-04
Identities = 18/45 (40%), Positives = 26/45 (57%)
Frame = -3
Query: 223 SKIAAYIESFRLGCPPHAGGGIGLERVVMLYLGLDNIRKTSMFPR 89
SK +E+ +G PPH G GL+R+VM+ G +IR FP+
Sbjct: 592 SKFGYLLEALDMGAPPHGGIAYGLDRMVMMLGGASSIRDVIAFPK 636
>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
family protein similar to SP|Q9RHV9 Lysyl-tRNA
synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
stearothermophilus}; contains Pfam profile: PF00152 tRNA
synthetases class II (D, K and N)
Length = 602
Score = 34.3 bits (75), Expect = 0.065
Identities = 14/39 (35%), Positives = 23/39 (58%)
Frame = -3
Query: 208 YIESFRLGCPPHAGGGIGLERVVMLYLGLDNIRKTSMFP 92
++ + G PP +G G+G++R+VML +IR FP
Sbjct: 558 FLTALEYGMPPASGMGLGIDRLVMLLTNSASIRDVIAFP 596
>At3g07420.1 68416.m00884 asparaginyl-tRNA synthetase 2, cytoplasmic
/ asparagine-tRNA ligase 2 (SYNC2) nearly identical to
SP|Q9SW95; HMM hit: tRNA synthetases class II
Length = 638
Score = 29.9 bits (64), Expect = 1.4
Identities = 14/49 (28%), Positives = 25/49 (51%)
Frame = -3
Query: 235 GVDTSKIAAYIESFRLGCPPHAGGGIGLERVVMLYLGLDNIRKTSMFPR 89
G K Y++ R G H+G + +E++++ GL +I+ FPR
Sbjct: 583 GFTREKFEWYLDLRRHGTVKHSGISLSMEQMLLYATGLPDIKDAIPFPR 631
>At5g52070.1 68418.m06464 agenet domain-containing protein contains
Pfam PF05641: Agenet domain
Length = 271
Score = 29.5 bits (63), Expect = 1.8
Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
Frame = +2
Query: 236 VEARALARR-SGSWIRWAPLSISSPRMNMSYELEYLGFP 349
VE ++ R G+W R L IS+ + + Y+ +YL FP
Sbjct: 14 VEVKSFIRGYRGAWFRCKILDISNEKGELRYKFKYLDFP 52
>At1g70640.1 68414.m08143 octicosapeptide/Phox/Bem1p (PB1)
domain-containing protein contains Pfam profile PF00564:
PB1 domain
Length = 174
Score = 29.1 bits (62), Expect = 2.4
Identities = 21/57 (36%), Positives = 27/57 (47%), Gaps = 1/57 (1%)
Frame = -2
Query: 464 PLR-TRSSWAGWCGPSTTRTSISWTNILSPSDPSTPCRIPGTLNIPIRTTCSCAARR 297
PL+ TR++ PSTT +S S + SP PSTP P + IR C R
Sbjct: 107 PLKSTRTTANSSPPPSTTSSSSSKSRSRSPPSPSTPETCPSCVERSIRNN-GCYVHR 162
>At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative /
lysine--tRNA ligase, putative similar to SP|Q43776
Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase)
{Lycopersicon esculentum}; contains Pfam profile
PF00152: tRNA synthetases class II (D, K and N)
Length = 626
Score = 28.7 bits (61), Expect = 3.2
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = -3
Query: 208 YIESFRLGCPPHAGGGIGLERVVMLYLGLDNIRKTSMFP 92
+ + G P G G+G++R+ ML NI++ FP
Sbjct: 567 FCNALEYGLAPTGGWGLGIDRLSMLLTDSLNIKEVLFFP 605
>At1g62020.1 68414.m06995 coatomer protein complex, subunit alpha,
putative contains Pfam PF00400: WD domain, G-beta
repeat; similar to Coatomer alpha subunit (Alpha-coat
protein) (Alpha-COP) (HEPCOP) (HEP-COP) (SP:P53621)
[Homo sapiens]
Length = 1216
Score = 28.7 bits (61), Expect = 3.2
Identities = 24/108 (22%), Positives = 46/108 (42%), Gaps = 6/108 (5%)
Frame = +1
Query: 292 KYLLAAHEHVVRIGIFRVPGIRHGVEGSDGERIFVQDI------EVRVVLGPHQPAQELL 453
KY+L H+ V F P + V G+D ++ + + EV + G ++
Sbjct: 197 KYVLEGHDRGVNWAAFH-PTLPLIVSGADDRQVKLWRMNETKAWEVDTLRGHMNNVSSVM 255
Query: 454 VRSGQVVLVSNRHSSFLQLWTACGNSSFQGRIQEFEGFHPVLLTHSRL 597
+ Q ++VSN +++W A + Q +E + F +L H +
Sbjct: 256 FHAKQDIIVSNSEDKSIRVWDATKRTGLQTFRREHDRFW-ILAVHPEM 302
>At3g19010.1 68416.m02413 oxidoreductase, 2OG-Fe(II) oxygenase
family protein contains similarity to flavonol synthase
(FLS) from [Solanum tuberosum] SP|Q41452, {Petunia
hybrida} SP|Q07512; contains Pfam profile PF03171:
oxidoreductase, 2OG-Fe(II) oxygenase family
Length = 349
Score = 28.3 bits (60), Expect = 4.2
Identities = 11/57 (19%), Positives = 25/57 (43%)
Frame = -1
Query: 492 VSVGDEDDLSTPDEKLLGRLVRAKYDTDFYILDKYPLAVRPFYTMPDPGNPKYSNSY 322
+ + D + + +++ R +Y F++L + + V+P + P NP Y
Sbjct: 262 MEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVEVKPLEELVSPENPPKYKGY 318
>At2g43920.1 68415.m05459 thiol methyltransferase, putative similar
to thiol methyltransferase 1 GI:14583119 from [Brassica
oleracea]
Length = 227
Score = 28.3 bits (60), Expect = 4.2
Identities = 14/39 (35%), Positives = 18/39 (46%), Gaps = 1/39 (2%)
Frame = -3
Query: 556 ILGSSPESWSFRRPSRVEGSWSVCWRRG-RPVHSGREAP 443
IL + E+ +F+ EG W CW G P GR P
Sbjct: 16 ILPTPEEAATFQPQVVAEGGWDKCWEDGVTPWDQGRATP 54
>At2g21390.1 68415.m02546 coatomer protein complex, subunit alpha,
putative contains Pfam PF00400: WD domain, G-beta
repeat; similar to Coatomer alpha subunit (Alpha-coat
protein) (Alpha-COP) (HEPCOP) (HEP-COP) (SP:P53621)
[Homo sapiens]
Length = 1218
Score = 28.3 bits (60), Expect = 4.2
Identities = 24/108 (22%), Positives = 46/108 (42%), Gaps = 6/108 (5%)
Frame = +1
Query: 292 KYLLAAHEHVVRIGIFRVPGIRHGVEGSDGERIFVQDI------EVRVVLGPHQPAQELL 453
KY+L H+ V F P + V G+D ++ + + EV + G ++
Sbjct: 197 KYVLEGHDRGVNWASFH-PTLPLIVSGADDRQVKLWRMNETKAWEVDTLRGHMNNVSSVM 255
Query: 454 VRSGQVVLVSNRHSSFLQLWTACGNSSFQGRIQEFEGFHPVLLTHSRL 597
+ Q ++VSN +++W A + Q +E + F +L H +
Sbjct: 256 FHAKQDIIVSNSEDKSIRVWDATKRTGIQTFRREHDRFW-ILAVHPEI 302
>At2g17340.1 68415.m02003 pantothenate kinase-related contains Pfam
domain, PF01937: Protein of unknown function; supported
by tandem duplication of pantothenate kinase -related
protein (TIGR_Ath1:At2g17320) [Arabidopsis thaliana]
Length = 367
Score = 27.5 bits (58), Expect = 7.4
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +1
Query: 388 IFVQDIEVRVVLGPHQPAQELLVRSGQVVLVSN 486
IFV + ++LG A+ELL R QVVL +N
Sbjct: 217 IFVDNSGADIILGILPFARELLRRGAQVVLAAN 249
>At4g28990.1 68417.m04143 RNA-binding protein-related contains weak
similarity to Swiss-Prot:Q01844 RNA-binding protein EWS
(EWS oncogene)(Ewing sarcoma breakpoint region 1
protein) [Homo sapiens]
Length = 347
Score = 27.1 bits (57), Expect = 9.8
Identities = 13/28 (46%), Positives = 13/28 (46%), Gaps = 1/28 (3%)
Frame = +3
Query: 399 GYRSPCRTWPAP-ACPGASRPEWTGRPR 479
GYRSP R WP P P W R R
Sbjct: 210 GYRSPPRGWPRDYPPPRHDHPTWRDRER 237
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,561,827
Number of Sequences: 28952
Number of extensions: 321914
Number of successful extensions: 1133
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1081
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1133
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1226538000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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