BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0640 (532 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC409.07c |wis1|spc2, smf2|MAP kinase kinase Wis1|Schizosaccha... 30 0.25 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 28 0.76 SPAC22E12.09c |krp1|krp|kexin|Schizosaccharomyces pombe|chr 1|||... 27 1.3 SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyc... 25 7.0 SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 25 9.3 >SPBC409.07c |wis1|spc2, smf2|MAP kinase kinase Wis1|Schizosaccharomyces pombe|chr 2|||Manual Length = 605 Score = 29.9 bits (64), Expect = 0.25 Identities = 20/60 (33%), Positives = 32/60 (53%), Gaps = 2/60 (3%) Frame = +2 Query: 5 IAGWYMAKKISGNA-L*MTAVAPGSMDELYSGGGRCDG-KNEMRYHLEQFLRALPMEHTV 178 I +Y A + G+ + M + GSMD+LY+GG + +G Y + Q L+ L EH + Sbjct: 378 IVDFYGAFFVEGSVFICMEYMDAGSMDKLYAGGIKDEGVLARTAYAVVQGLKTLKEEHNI 437 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 28.3 bits (60), Expect = 0.76 Identities = 12/30 (40%), Positives = 18/30 (60%) Frame = +2 Query: 128 RYHLEQFLRALPMEHTVQNAEGTEVPPQTQ 217 RY LEQ L+ P+EH + E + PP+ + Sbjct: 85 RYGLEQQLKTNPLEHPILITEPFDNPPENR 114 >SPAC22E12.09c |krp1|krp|kexin|Schizosaccharomyces pombe|chr 1|||Manual Length = 709 Score = 27.5 bits (58), Expect = 1.3 Identities = 12/27 (44%), Positives = 15/27 (55%) Frame = +1 Query: 34 LWKRTVNDRSGSR*YG*TLLRWRAVRW 114 +WK VNDRSG + G T W+ W Sbjct: 558 VWKLLVNDRSGGKHEG-TFENWQLALW 583 >SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 25.0 bits (52), Expect = 7.0 Identities = 9/24 (37%), Positives = 12/24 (50%) Frame = -3 Query: 329 APIYGPGLPNTSFFHLTPYRGGPF 258 AP+Y P NT+ + Y PF Sbjct: 131 APLYSPSYNNTNLLYNFSYNANPF 154 >SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like protein modifier Ned8|Schizosaccharomyces pombe|chr 2|||Manual Length = 78 Score = 24.6 bits (51), Expect = 9.3 Identities = 10/27 (37%), Positives = 13/27 (48%) Frame = +2 Query: 62 VAPGSMDELYSGGGRCDGKNEMRYHLE 142 + P +Y+G D KN YHLE Sbjct: 36 IPPSQQRLIYAGKQMADDKNAESYHLE 62 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,701,394 Number of Sequences: 5004 Number of extensions: 62391 Number of successful extensions: 164 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 158 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 164 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 218398248 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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