Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--0600
(676 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 140 2e-34
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 130 2e-31
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 80 3e-16
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 69 5e-13
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.7
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 5.7
SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual 25 7.6
SPAC458.07 |tfa1|SPAPYUG7.01|transcription factor TFIIE alpha su... 25 7.6
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 140 bits (338), Expect = 2e-34
Identities = 60/85 (70%), Positives = 69/85 (81%)
Frame = +1
Query: 1 VGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFV 180
VGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK+VPR+++V
Sbjct: 9 VGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYV 68
Query: 181 DLEPTVVDEVRTGTYRQLFHPEQLI 255
DLEP V+D+VRTG YR LFHPEQLI
Sbjct: 69 DLEPNVIDQVRTGPYRDLFHPEQLI 93
Score = 116 bits (280), Expect = 2e-27
Identities = 50/81 (61%), Positives = 65/81 (80%)
Frame = +3
Query: 255 TGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLL 434
TGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH LL
Sbjct: 94 TGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALL 153
Query: 435 MERLSVDYGKKSKLEFAIYPA 497
+ERL+++Y KKSKL+F++YPA
Sbjct: 154 LERLAMEYTKKSKLQFSVYPA 174
Score = 90.2 bits (214), Expect = 2e-19
Identities = 38/49 (77%), Positives = 44/49 (89%)
Frame = +2
Query: 509 TAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNL 655
T+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NL
Sbjct: 179 TSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENL 227
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 130 bits (313), Expect = 2e-31
Identities = 60/90 (66%), Positives = 70/90 (77%), Gaps = 5/90 (5%)
Frame = +1
Query: 1 VGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHVP 165
VGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSETG GK VP
Sbjct: 9 VGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVP 67
Query: 166 RAVFVDLEPTVVDEVRTGTYRQLFHPEQLI 255
R+++VDLEP V+D+VRTG Y+ LFHPEQ++
Sbjct: 68 RSIYVDLEPNVIDQVRTGPYKDLFHPEQMV 97
Score = 119 bits (286), Expect = 4e-28
Identities = 50/81 (61%), Positives = 66/81 (81%)
Frame = +3
Query: 255 TGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLL 434
TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH LL
Sbjct: 98 TGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALL 157
Query: 435 MERLSVDYGKKSKLEFAIYPA 497
+ERL+++YGKKS L+F++YPA
Sbjct: 158 LERLNMEYGKKSNLQFSVYPA 178
Score = 95.9 bits (228), Expect = 5e-21
Identities = 41/49 (83%), Positives = 45/49 (91%)
Frame = +2
Query: 509 TAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNL 655
T+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NL
Sbjct: 183 TSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENL 231
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 80.2 bits (189), Expect = 3e-16
Identities = 35/87 (40%), Positives = 56/87 (64%)
Frame = +1
Query: 4 GQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 183
GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ +++PRA+ +D
Sbjct: 11 GQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILID 68
Query: 184 LEPTVVDEVRTGTYRQLFHPEQLILVR 264
LEP VV+ + + TY L++PE +++ +
Sbjct: 69 LEPRVVNNILSDTYGSLYNPENILITK 95
Score = 42.3 bits (95), Expect = 6e-05
Identities = 23/75 (30%), Positives = 38/75 (50%)
Frame = +3
Query: 270 AANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 449
A NN+A G Y+ + I + ++D I + AD L+GF + H L+ERL+
Sbjct: 100 AGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLN 158
Query: 450 VDYGKKSKLEFAIYP 494
Y KK ++++P
Sbjct: 159 DRYPKKIIQTYSVFP 173
Score = 33.9 bits (74), Expect = 0.022
Identities = 15/44 (34%), Positives = 24/44 (54%)
Frame = +2
Query: 515 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTY 646
VV+PYNS+L ++D ++DN A+ I L + PT+
Sbjct: 182 VVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTF 225
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 69.3 bits (162), Expect = 5e-13
Identities = 34/84 (40%), Positives = 46/84 (54%)
Frame = +1
Query: 4 GQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 183
GQ G Q+G A W EHG+ G T + N +F+E GK+VPRAV VD
Sbjct: 10 GQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVD 67
Query: 184 LEPTVVDEVRTGTYRQLFHPEQLI 255
LEP +D V++G + LF P+ +I
Sbjct: 68 LEPGTMDAVKSGKFGNLFRPDNII 91
Score = 60.9 bits (141), Expect = 2e-10
Identities = 28/80 (35%), Positives = 43/80 (53%)
Frame = +3
Query: 258 GKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLM 437
G+ A N +A+GHYT G E+ D VLD +R+ A+ C LQGF + H LL+
Sbjct: 93 GQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLL 152
Query: 438 ERLSVDYGKKSKLEFAIYPA 497
++ +Y + F++ PA
Sbjct: 153 SKIREEYPDRMMATFSVAPA 172
Score = 50.0 bits (114), Expect = 3e-07
Identities = 23/55 (41%), Positives = 32/55 (58%)
Frame = +2
Query: 491 PRASGFTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNL 655
P VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P+Y +L
Sbjct: 171 PAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDL 225
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 5.7
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 56 STASSLMARCPQTRPSGVETILSTLSSARPELAST 160
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 5.7
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 75 WPDAHRQDHRGWRRFFQHFLQR 140
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 483
Score = 25.4 bits (53), Expect = 7.6
Identities = 11/29 (37%), Positives = 14/29 (48%)
Frame = +2
Query: 485 HLPRASGFTAVVEPYNSILTTHTTLEHSD 571
HLP ASG + PY + TT +D
Sbjct: 155 HLPNASGSSVYQPPYTPFRSVSTTSSSND 183
>SPAC458.07 |tfa1|SPAPYUG7.01|transcription factor TFIIE alpha
subunit Tfa1 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 448
Score = 25.4 bits (53), Expect = 7.6
Identities = 9/22 (40%), Positives = 12/22 (54%)
Frame = +3
Query: 210 PHWHIQTVVSSRTTYTGKEDAA 275
P WH + +S T G +DAA
Sbjct: 280 PEWHATSTISGSITRAGAKDAA 301
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,795,735
Number of Sequences: 5004
Number of extensions: 57497
Number of successful extensions: 222
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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