Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ce--0556
(588 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 239 2e-64
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 133 2e-32
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 121 7e-29
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 96 3e-21
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 72 5e-14
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 64 1e-11
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 60 3e-10
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 47 2e-06
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.071
SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Sc... 29 0.50
SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 28 0.88
SPBC244.01c |sid4||SIN component scaffold protein Sid4 |Schizosa... 28 0.88
SPCC1827.05c |||nucleolar RNA-binding protein NIFK |Schizosaccha... 27 1.5
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 27 2.0
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 27 2.0
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.5
SPAC1B1.02c |||NAD/NADH kinase |Schizosaccharomyces pombe|chr 1|... 26 4.7
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 25 6.2
SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual 25 6.2
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 239 bits (586), Expect = 2e-64
Identities = 111/131 (84%), Positives = 115/131 (87%)
Frame = +1
Query: 172 DDAPRAVFPSIVGRPRHQGVMVGMGQKDLM*EMRHMSKRGILTLKYPIEHGIVTNWDDME 351
DDAPRAVFPSIVGRPRH G+MVGMGQKD SKRGILTLKYPIEHGIV NWDDME
Sbjct: 24 DDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDME 83
Query: 352 KIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYCRLQAVLSLY 531
KIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQI+FETFN PA Y +QAVLSLY
Sbjct: 84 KIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLY 143
Query: 532 AFGRTTGIVLD 564
A GRTTGIVLD
Sbjct: 144 ASGRTTGIVLD 154
Score = 49.2 bits (112), Expect = 4e-07
Identities = 26/59 (44%), Positives = 33/59 (55%)
Frame = +3
Query: 111 KLPRLVVDNGSGMCKAGFAGG*CSSRRVPLDRGKAPPSGRDGRYGTEGSYVGDEAHEQK 287
++ LV+DNGSGMCKAGFAG P G+ G G + SYVGDEA ++
Sbjct: 4 EIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSKR 62
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 133 bits (321), Expect = 2e-32
Identities = 63/132 (47%), Positives = 87/132 (65%), Gaps = 1/132 (0%)
Frame = +1
Query: 172 DDAPRAVFPSIVGRPRHQGVMVGMGQKDLM*EMRHMSKRGILTLKYPIEHGIVTNWDDME 351
DD P+ +FP+ VGR +H+ VM QKD+ + RG+L ++ PIE GI+ NW DME
Sbjct: 28 DDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDME 87
Query: 352 KIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYCRLQAVLSL 528
+IW + + ++ L PEEHP+LLTE PL N+EK+ + +ET N PA+ LQ VL+L
Sbjct: 88 EIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLAL 147
Query: 529 YAFGRTTGIVLD 564
YA RTTGIVL+
Sbjct: 148 YASARTTGIVLE 159
Score = 29.1 bits (62), Expect = 0.50
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +3
Query: 123 LVVDNGSGMCKAGFAG 170
+ +DNGSG KAGFAG
Sbjct: 12 ICIDNGSGFIKAGFAG 27
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 121 bits (292), Expect = 7e-29
Identities = 58/134 (43%), Positives = 90/134 (67%), Gaps = 2/134 (1%)
Frame = +1
Query: 169 EDDAPRAVFPSIVGRP--RHQGVMVGMGQKDLM*EMRHMSKRGILTLKYPIEHGIVTNWD 342
+D+ PR FPSIVGRP R + + KD+M + R +L +KYP+E+GI+ +++
Sbjct: 21 KDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFE 80
Query: 343 DMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYCRLQAVL 522
+M ++W +TF+ +L++ P +LLTE P+NP ANREKM + MFE + +Y +QAVL
Sbjct: 81 EMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQAVL 140
Query: 523 SLYAFGRTTGIVLD 564
SLYA G ++G+V+D
Sbjct: 141 SLYAQGLSSGVVVD 154
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 96.3 bits (229), Expect = 3e-21
Identities = 46/94 (48%), Positives = 58/94 (61%)
Frame = +1
Query: 298 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 477
+L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE
Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148
Query: 478 TFNTPAMYCRLQAVLSLYAFGRTTGIVLDLRPTG 579
+FN +Y +QAVL+L A T+ V D TG
Sbjct: 149 SFNCAGLYIAVQAVLAL-AASWTSSKVTDRSLTG 181
Score = 25.0 bits (52), Expect = 8.2
Identities = 8/16 (50%), Positives = 13/16 (81%)
Frame = +3
Query: 123 LVVDNGSGMCKAGFAG 170
+++DNG+G K G+AG
Sbjct: 8 IIMDNGTGYSKLGYAG 23
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 72.1 bits (169), Expect = 5e-14
Identities = 42/132 (31%), Positives = 64/132 (48%)
Frame = +1
Query: 172 DDAPRAVFPSIVGRPRHQGVMVGMGQKDLM*EMRHMSKRGILTLKYPIEHGIVTNWDDME 351
+D P+ V PS G G++ E + S G + +K I +G V NWD
Sbjct: 30 EDIPKCVLPSYCGE-------FSDGRRLFGEEYIYKSNPG-MEIKNAIRNGWVENWDVTV 81
Query: 352 KIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYCRLQAVLSLY 531
+W + +L+ P EHP+L+TE NP NR K + MFE+ PA Y Q + +
Sbjct: 82 DLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMFESLRCPATYLAKQETCAAF 141
Query: 532 AFGRTTGIVLDL 567
A G+ T ++D+
Sbjct: 142 ASGKGTACLVDI 153
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 64.1 bits (149), Expect = 1e-11
Identities = 31/88 (35%), Positives = 53/88 (60%), Gaps = 2/88 (2%)
Frame = +1
Query: 310 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNT 489
P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N
Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144
Query: 490 PAMYCRLQAVLSLYAFGR--TTGIVLDL 567
P++ + + S Y + ++GIVL+L
Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSSGIVLNL 172
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 59.7 bits (138), Expect = 3e-10
Identities = 24/83 (28%), Positives = 48/83 (57%)
Frame = +1
Query: 319 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAM 498
+GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA
Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133
Query: 499 YCRLQAVLSLYAFGRTTGIVLDL 567
Y QAV +A ++T +++D+
Sbjct: 134 YLTKQAVCVAFANSKSTALIVDI 156
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 46.8 bits (106), Expect = 2e-06
Identities = 27/90 (30%), Positives = 42/90 (46%), Gaps = 4/90 (4%)
Frame = +1
Query: 310 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 477
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 478 TFNTPAMYCRLQAVLSLYAFGRTTGIVLDL 567
P + ++ LYA G G+V+D+
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDI 201
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 31.9 bits (69), Expect = 0.071
Identities = 17/55 (30%), Positives = 31/55 (56%), Gaps = 2/55 (3%)
Frame = +1
Query: 370 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAMYCRLQAVLSL 528
+Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + + A+ L
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQHL 1453
>SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 29.1 bits (62), Expect = 0.50
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = -1
Query: 306 FEGQDTSFAHVPHLLHKIL 250
FEG +T FA++ HLLH +L
Sbjct: 544 FEGLETFFAYIVHLLHGVL 562
>SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1038
Score = 28.3 bits (60), Expect = 0.88
Identities = 17/56 (30%), Positives = 25/56 (44%), Gaps = 3/56 (5%)
Frame = +3
Query: 429 PQPQGQQREDD---PDHVRNIQHARHVLSPPSRALAVRVRSYHRYRAGPPADRCLP 587
PQ Q R D ++ R + R +S P+ LA+R S+ RY+ LP
Sbjct: 176 PQSQDDNRVSDIRQMENRRELNVKRRSVSSPAEDLAIRPLSFSRYKKAEEIPESLP 231
>SPBC244.01c |sid4||SIN component scaffold protein Sid4
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 660
Score = 28.3 bits (60), Expect = 0.88
Identities = 12/45 (26%), Positives = 22/45 (48%)
Frame = +3
Query: 183 SRRVPLDRGKAPPSGRDGRYGTEGSYVGDEAHEQKRYPDPQIPHR 317
S +VP R PP R +Y E ++ + Q+ + P++ +R
Sbjct: 207 SLQVPPSRNVPPPVTRPNQYNPEPNFSLSSGYPQQHFSQPELQNR 251
>SPCC1827.05c |||nucleolar RNA-binding protein NIFK
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 276
Score = 27.5 bits (58), Expect = 1.5
Identities = 10/29 (34%), Positives = 16/29 (55%)
Frame = -1
Query: 354 LLHVIPVSDDSVFDGVFEGQDTSFAHVPH 268
LL + +D V + +F+G D F +PH
Sbjct: 175 LLQCKVIPEDQVHENMFKGADVPFKRIPH 203
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 27.1 bits (57), Expect = 2.0
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = +3
Query: 123 LVVDNGSGMCKAGFAGG 173
+V+DNG+ KAGFAGG
Sbjct: 8 IVLDNGAYHIKAGFAGG 24
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 27.1 bits (57), Expect = 2.0
Identities = 12/39 (30%), Positives = 16/39 (41%)
Frame = -3
Query: 253 PSVPYRPSRPDGGAFPRSRGTRREEHHPPAKPALHIPEP 137
P VP P RP P + + P AL +P+P
Sbjct: 539 PEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSVPQP 577
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 3.5
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +1
Query: 412 LLTEAPLNPKANREKMTQIMFETFN 486
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPAC1B1.02c |||NAD/NADH kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 537
Score = 25.8 bits (54), Expect = 4.7
Identities = 16/41 (39%), Positives = 18/41 (43%)
Frame = +3
Query: 453 EDDPDHVRNIQHARHVLSPPSRALAVRVRSYHRYRAGPPAD 575
E PD N+Q + V SPPS VRV R R D
Sbjct: 55 EPSPDEASNLQELKFVSSPPS----VRVPRSRRNRRNSRVD 91
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 25.4 bits (53), Expect = 6.2
Identities = 14/44 (31%), Positives = 23/44 (52%)
Frame = +1
Query: 181 PRAVFPSIVGRPRHQGVMVGMGQKDLM*EMRHMSKRGILTLKYP 312
PRA F ++ P H G+++ M KD + MS +G + + P
Sbjct: 506 PRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547
>SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual
Length = 935
Score = 25.4 bits (53), Expect = 6.2
Identities = 15/44 (34%), Positives = 24/44 (54%), Gaps = 4/44 (9%)
Frame = -2
Query: 362 CQIFSM----SSQLVTIPCSMGYLRVRIPLLLMCLISYIRSFCP 243
C +FS+ SS++V + + IP LL+C+ YI+S P
Sbjct: 465 CFVFSLNIEPSSKIVKPVSVVKRVAFNIPFLLICIFFYIQSSPP 508
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,652,569
Number of Sequences: 5004
Number of extensions: 58664
Number of successful extensions: 149
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 254167452
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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