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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ce--0539
         (469 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   129   2e-31
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   127   1e-30
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    80   2e-16
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    58   5e-10
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    27   1.9  
SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos...    26   3.3  
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr...    26   3.3  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   3.3  
SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosac...    25   7.6  
SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c...    25   7.6  
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom...    25   7.6  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  129 bits (312), Expect = 2e-31
 Identities = 55/71 (77%), Positives = 64/71 (90%)
 Frame = +1

Query: 256 DLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDIIRKLADQ 435
           DLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D IR++AD 
Sbjct: 69  DLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADN 128

Query: 436 CTGLQGFLIFH 468
           C+GLQGFL+FH
Sbjct: 129 CSGLQGFLVFH 139



 Score =  107 bits (256), Expect = 1e-24
 Identities = 45/67 (67%), Positives = 53/67 (79%)
 Frame = +2

Query: 53  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 232
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 233 HVPRAVF 253
           +VPR+++
Sbjct: 61  YVPRSIY 67


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  127 bits (306), Expect = 1e-30
 Identities = 51/71 (71%), Positives = 65/71 (91%)
 Frame = +1

Query: 256 DLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDIIRKLADQ 435
           DLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+ IR++AD 
Sbjct: 73  DLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADN 132

Query: 436 CTGLQGFLIFH 468
           C+GLQGFL+FH
Sbjct: 133 CSGLQGFLVFH 143



 Score =   99 bits (238), Expect = 2e-22
 Identities = 49/72 (68%), Positives = 54/72 (75%), Gaps = 5/72 (6%)
 Frame = +2

Query: 53  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 217
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 218 TGAGKHVPRAVF 253
           TG GK VPR+++
Sbjct: 60  TGQGKFVPRSIY 71


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 79.8 bits (188), Expect = 2e-16
 Identities = 34/73 (46%), Positives = 49/73 (67%)
 Frame = +1

Query: 250 LRDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDIIRKLA 429
           L DLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD++R+ A
Sbjct: 65  LVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREA 124

Query: 430 DQCTGLQGFLIFH 468
           + C  LQGF + H
Sbjct: 125 EACDALQGFQLTH 137



 Score = 49.6 bits (113), Expect = 2e-07
 Identities = 25/66 (37%), Positives = 33/66 (50%)
 Frame = +2

Query: 53  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 232
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 233 HVPRAV 250
           +VPRAV
Sbjct: 59  YVPRAV 64


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 58.4 bits (135), Expect = 5e-10
 Identities = 33/96 (34%), Positives = 53/96 (55%), Gaps = 1/96 (1%)
 Frame = +2

Query: 56  RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 235
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 236 VPRAVFAIXXXXXXMRSALAHT-DSCFIQNNLLLVR 340
           +PRA+  I      + + L+ T  S +   N+L+ +
Sbjct: 61  IPRAIL-IDLEPRVVNNILSDTYGSLYNPENILITK 95


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 26.6 bits (56), Expect = 1.9
 Identities = 17/41 (41%), Positives = 22/41 (53%)
 Frame = +3

Query: 132 STASSLMARCPQTRPSGVETILSTLSSARPELASTYPVLSS 254
           ST SSL +    ++PS   T  ST SSA P   S+  + SS
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSSSYLSSS 213


>SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 400

 Score = 25.8 bits (54), Expect = 3.3
 Identities = 10/23 (43%), Positives = 15/23 (65%)
 Frame = -1

Query: 106 ITDLDSGLANVYRDALTHFNLSF 38
           +T +D GLA  YRD  TH ++ +
Sbjct: 146 VTMIDFGLAKKYRDFKTHVHIPY 168


>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1024

 Score = 25.8 bits (54), Expect = 3.3
 Identities = 11/38 (28%), Positives = 17/38 (44%)
 Frame = -2

Query: 288 ADLINNSRFKIAKTARGTCLPAPVSLKKVLKESSPPPM 175
           AD+IN SR+ I + +  T     + L        P P+
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQPI 492


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 3.3
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +1

Query: 151 WPDAHRQDHRGWRRFFQHFLQR 216
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


>SPBC3H7.15 |hhp1||serine/threonine protein kinase
           Hhp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 365

 Score = 24.6 bits (51), Expect = 7.6
 Identities = 14/42 (33%), Positives = 21/42 (50%), Gaps = 1/42 (2%)
 Frame = -1

Query: 160 HLAIRLDAVLQTV-KLPAGITDLDSGLANVYRDALTHFNLSF 38
           H  I+ D  L  + K    +  +D GLA  YRD  TH ++ +
Sbjct: 128 HRDIKPDNFLMGIGKRGNQVNIIDFGLAKKYRDHKTHLHIPY 169


>SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 649

 Score = 24.6 bits (51), Expect = 7.6
 Identities = 10/23 (43%), Positives = 14/23 (60%)
 Frame = +1

Query: 337 KEDAANNYARGHYTIGKEIVDLV 405
           +E  ANN+ RGH  I K+  D +
Sbjct: 599 RERKANNFFRGHIEIDKKTKDFL 621


>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 534

 Score = 24.6 bits (51), Expect = 7.6
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = +3

Query: 75  TLAKPESRSVMPAGSFTVWSTASSLMARCP 164
           +L+ P SR++ P  S +  STASSL    P
Sbjct: 181 SLSPPSSRALKPIHSLSNPSTASSLEPSSP 210


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,075,311
Number of Sequences: 5004
Number of extensions: 42685
Number of successful extensions: 135
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 178394480
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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