BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0539 (469 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 129 2e-31 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 127 1e-30 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 80 2e-16 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 58 5e-10 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 1.9 SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 26 3.3 SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 26 3.3 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 3.3 SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosac... 25 7.6 SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c... 25 7.6 SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 7.6 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 129 bits (312), Expect = 2e-31 Identities = 55/71 (77%), Positives = 64/71 (90%) Frame = +1 Query: 256 DLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDIIRKLADQ 435 DLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D IR++AD Sbjct: 69 DLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADN 128 Query: 436 CTGLQGFLIFH 468 C+GLQGFL+FH Sbjct: 129 CSGLQGFLVFH 139 Score = 107 bits (256), Expect = 1e-24 Identities = 45/67 (67%), Positives = 53/67 (79%) Frame = +2 Query: 53 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 232 MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 233 HVPRAVF 253 +VPR+++ Sbjct: 61 YVPRSIY 67 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 127 bits (306), Expect = 1e-30 Identities = 51/71 (71%), Positives = 65/71 (91%) Frame = +1 Query: 256 DLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDIIRKLADQ 435 DLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+ IR++AD Sbjct: 73 DLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADN 132 Query: 436 CTGLQGFLIFH 468 C+GLQGFL+FH Sbjct: 133 CSGLQGFLVFH 143 Score = 99 bits (238), Expect = 2e-22 Identities = 49/72 (68%), Positives = 54/72 (75%), Gaps = 5/72 (6%) Frame = +2 Query: 53 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 217 MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59 Query: 218 TGAGKHVPRAVF 253 TG GK VPR+++ Sbjct: 60 TGQGKFVPRSIY 71 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 79.8 bits (188), Expect = 2e-16 Identities = 34/73 (46%), Positives = 49/73 (67%) Frame = +1 Query: 250 LRDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDIIRKLA 429 L DLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD++R+ A Sbjct: 65 LVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREA 124 Query: 430 DQCTGLQGFLIFH 468 + C LQGF + H Sbjct: 125 EACDALQGFQLTH 137 Score = 49.6 bits (113), Expect = 2e-07 Identities = 25/66 (37%), Positives = 33/66 (50%) Frame = +2 Query: 53 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 232 MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58 Query: 233 HVPRAV 250 +VPRAV Sbjct: 59 YVPRAV 64 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 58.4 bits (135), Expect = 5e-10 Identities = 33/96 (34%), Positives = 53/96 (55%), Gaps = 1/96 (1%) Frame = +2 Query: 56 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 235 RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++ Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60 Query: 236 VPRAVFAIXXXXXXMRSALAHT-DSCFIQNNLLLVR 340 +PRA+ I + + L+ T S + N+L+ + Sbjct: 61 IPRAIL-IDLEPRVVNNILSDTYGSLYNPENILITK 95 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 26.6 bits (56), Expect = 1.9 Identities = 17/41 (41%), Positives = 22/41 (53%) Frame = +3 Query: 132 STASSLMARCPQTRPSGVETILSTLSSARPELASTYPVLSS 254 ST SSL + ++PS T ST SSA P S+ + SS Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSSSYLSSS 213 >SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 400 Score = 25.8 bits (54), Expect = 3.3 Identities = 10/23 (43%), Positives = 15/23 (65%) Frame = -1 Query: 106 ITDLDSGLANVYRDALTHFNLSF 38 +T +D GLA YRD TH ++ + Sbjct: 146 VTMIDFGLAKKYRDFKTHVHIPY 168 >SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr 3|||Manual Length = 1024 Score = 25.8 bits (54), Expect = 3.3 Identities = 11/38 (28%), Positives = 17/38 (44%) Frame = -2 Query: 288 ADLINNSRFKIAKTARGTCLPAPVSLKKVLKESSPPPM 175 AD+IN SR+ I + + T + L P P+ Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQPI 492 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 3.3 Identities = 13/22 (59%), Positives = 13/22 (59%) Frame = +1 Query: 151 WPDAHRQDHRGWRRFFQHFLQR 216 W A R D R R FQHFLQR Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611 >SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 365 Score = 24.6 bits (51), Expect = 7.6 Identities = 14/42 (33%), Positives = 21/42 (50%), Gaps = 1/42 (2%) Frame = -1 Query: 160 HLAIRLDAVLQTV-KLPAGITDLDSGLANVYRDALTHFNLSF 38 H I+ D L + K + +D GLA YRD TH ++ + Sbjct: 128 HRDIKPDNFLMGIGKRGNQVNIIDFGLAKKYRDHKTHLHIPY 169 >SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 24.6 bits (51), Expect = 7.6 Identities = 10/23 (43%), Positives = 14/23 (60%) Frame = +1 Query: 337 KEDAANNYARGHYTIGKEIVDLV 405 +E ANN+ RGH I K+ D + Sbjct: 599 RERKANNFFRGHIEIDKKTKDFL 621 >SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pombe|chr 3|||Manual Length = 534 Score = 24.6 bits (51), Expect = 7.6 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = +3 Query: 75 TLAKPESRSVMPAGSFTVWSTASSLMARCP 164 +L+ P SR++ P S + STASSL P Sbjct: 181 SLSPPSSRALKPIHSLSNPSTASSLEPSSP 210 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,075,311 Number of Sequences: 5004 Number of extensions: 42685 Number of successful extensions: 135 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 125 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 132 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 178394480 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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