BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ce--0536 (595 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces p... 29 0.51 SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe... 28 1.2 SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 25 6.3 SPBC106.17c |cys2||O-acetyltransferase |Schizosaccharomyces pomb... 25 6.3 >SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 417 Score = 29.1 bits (62), Expect = 0.51 Identities = 15/34 (44%), Positives = 18/34 (52%) Frame = -2 Query: 510 GVVWGDGTSSGTVFDS*CTLRSSPGTLKTVRSST 409 G+ GDG S V + C+ SPG LKT ST Sbjct: 192 GIQIGDGAGSSCVEGAYCSYACSPGMLKTQWPST 225 >SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 317 Score = 27.9 bits (59), Expect = 1.2 Identities = 20/75 (26%), Positives = 36/75 (48%) Frame = -1 Query: 247 SSSFKGSLVCSTHFPLLMCTLTSSSKSMFGSAVLALFCLLLSEPGPSAGGTEWSCFFRSC 68 S S+ G++ ++ P ++ T TSSS + S+ S PS+ ++ S +S Sbjct: 120 SLSYSGTISSTSIAPSMIGTRTSSSYFITSSSSTPSSSSSSSSSSPSSSSSKSSSSSKSS 179 Query: 67 TCSMSSGQRSGCAGS 23 + S SS + S + S Sbjct: 180 SSSSSSSKSSSSSSS 194 >SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1049 Score = 25.4 bits (53), Expect = 6.3 Identities = 9/29 (31%), Positives = 16/29 (55%) Frame = +1 Query: 238 RMTKLNWRAYARRSVNIWGSVRDWYCSSQ 324 R+ + WR Y R+S+N + + YC + Sbjct: 260 RLNPVVWRTYLRKSLNSYVDKLEVYCQKR 288 >SPBC106.17c |cys2||O-acetyltransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 504 Score = 25.4 bits (53), Expect = 6.3 Identities = 22/65 (33%), Positives = 26/65 (40%), Gaps = 7/65 (10%) Frame = +3 Query: 243 DEIKLEGVCASVGQHLGVCQRLVLQLPTLLARGQWGVPGGRPRLHAAS-------PQRLD 401 D +K+E + ASVG LG Q L L G+ G R H S Q L Sbjct: 207 DHLKIEKLYASVGSSLGGMQSLTLGALAPHRVGRIASISGGARSHPYSIALRFTQRQILM 266 Query: 402 TSPYW 416 PYW Sbjct: 267 NDPYW 271 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,389,668 Number of Sequences: 5004 Number of extensions: 50127 Number of successful extensions: 168 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 160 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 168 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 258201856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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