Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ce--0480
         (708 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   136   4e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   134   9e-33
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    82   9e-17
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    56   4e-09
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr...    28   1.1  
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   6.1  
SPBC3B8.01c |arh1||NADPH-adrenodoxin reductase Arh1 |Schizosacch...    26   6.1  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   6.1  
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom...    25   8.0  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  136 bits (328), Expect = 4e-33
 Identities = 56/75 (74%), Positives = 69/75 (92%)
 Frame = +1

Query: 256 AVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRK 435
           +++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR+
Sbjct: 65  SIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRR 124

Query: 436 LADQCTGLQGFLIFH 480
           +AD C+GLQGFL+FH
Sbjct: 125 IADNCSGLQGFLVFH 139



 Score =  102 bits (244), Expect = 6e-23
 Identities = 44/63 (69%), Positives = 49/63 (77%)
 Frame = +2

Query: 65  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 244
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 245 HVP 253
           +VP
Sbjct: 61  YVP 63



 Score = 66.9 bits (156), Expect = 3e-12
 Identities = 35/66 (53%), Positives = 48/66 (72%), Gaps = 1/66 (1%)
 Frame = +3

Query: 513 SLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYN-FLFSPPTQTLEHF*LVLSWSTNE 689
           +LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYN  L +  T  L     ++    NE
Sbjct: 151 ALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVD---NE 207

Query: 690 TIYDIC 707
           + YDIC
Sbjct: 208 SCYDIC 213


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  134 bits (325), Expect = 9e-33
 Identities = 53/75 (70%), Positives = 70/75 (93%)
 Frame = +1

Query: 256 AVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRK 435
           +++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR+
Sbjct: 69  SIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRR 128

Query: 436 LADQCTGLQGFLIFH 480
           +AD C+GLQGFL+FH
Sbjct: 129 MADNCSGLQGFLVFH 143



 Score = 95.1 bits (226), Expect = 9e-21
 Identities = 48/68 (70%), Positives = 50/68 (73%), Gaps = 5/68 (7%)
 Frame = +2

Query: 65  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 229
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 230 TGAGKHVP 253
           TG GK VP
Sbjct: 60  TGQGKFVP 67



 Score = 68.9 bits (161), Expect = 7e-13
 Identities = 34/65 (52%), Positives = 47/65 (72%)
 Frame = +3

Query: 513 SLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNFLFSPPTQTLEHF*LVLSWSTNET 692
           +LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYN + +    TL++         NE 
Sbjct: 155 ALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHA-TLDNSDCTF-MVDNEA 212

Query: 693 IYDIC 707
            YDIC
Sbjct: 213 CYDIC 217


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 81.8 bits (193), Expect = 9e-17
 Identities = 36/75 (48%), Positives = 50/75 (66%)
 Frame = +1

Query: 256 AVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRK 435
           AV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD +R+
Sbjct: 63  AVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRR 122

Query: 436 LADQCTGLQGFLIFH 480
            A+ C  LQGF + H
Sbjct: 123 EAEACDALQGFQLTH 137



 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 22/63 (34%), Positives = 30/63 (47%)
 Frame = +2

Query: 65  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 244
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 245 HVP 253
           +VP
Sbjct: 59  YVP 61



 Score = 35.5 bits (78), Expect = 0.008
 Identities = 15/40 (37%), Positives = 24/40 (60%)
 Frame = +3

Query: 513 SLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNFLFS 632
           +LL+ ++  +Y  +    F++ PAP+ S  VVEPYN   S
Sbjct: 149 TLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLS 188


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 56.4 bits (130), Expect = 4e-09
 Identities = 28/77 (36%), Positives = 46/77 (59%), Gaps = 2/77 (2%)
 Frame = +1

Query: 256 AVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDRI 429
           A+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++D I
Sbjct: 64  AILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMI 122

Query: 430 RKLADQCTGLQGFLIFH 480
            + AD    L+GF + H
Sbjct: 123 DREADGSDSLEGFSLLH 139



 Score = 52.4 bits (120), Expect = 6e-08
 Identities = 25/62 (40%), Positives = 37/62 (59%)
 Frame = +2

Query: 68  RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 247
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 248 VP 253
           +P
Sbjct: 61  IP 62



 Score = 35.1 bits (77), Expect = 0.010
 Identities = 20/47 (42%), Positives = 28/47 (59%), Gaps = 1/47 (2%)
 Frame = +3

Query: 513 SLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNFLFSPPTQTL 650
           S L+ERL+  Y KK    ++++P  Q VS  VV+PYN L +    TL
Sbjct: 151 SFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTL 197


>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1024

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 13/38 (34%), Positives = 17/38 (44%)
 Frame = -1

Query: 300 ADLINNSRFKIDEDSRGTCLPAPVSLKKVLKESSPPPM 187
           AD+IN SR+ ID  S  T     + L        P P+
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQPI 492


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +3

Query: 144 STASSLMARCPQTRPSGVETILSTLSSARPELAST 248
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPBC3B8.01c |arh1||NADPH-adrenodoxin reductase Arh1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 469

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 16/64 (25%), Positives = 31/64 (48%), Gaps = 6/64 (9%)
 Frame = -1

Query: 342 NKLFWMKQLSVCASADLINNSRFKI------DEDSRGTCLPAPVSLKKVLKESSPPPMVL 181
           N  F +  + +      + N RF+I      D +S+ T +PA + ++ +  +S P P + 
Sbjct: 287 NLEFGLTPVEILGHKGNVENVRFQITDSIRTDAESKFTTIPAQLFIRSIGYKSMPLPGMK 346

Query: 180 SVGI 169
            VG+
Sbjct: 347 DVGV 350


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +1

Query: 163 WPDAHRQDHRGWRRFFQHFLQR 228
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 534

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 15/41 (36%), Positives = 21/41 (51%)
 Frame = +3

Query: 54  LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 176
           L+S        +L+ P SR++ P  S +  STASSL    P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,069,897
Number of Sequences: 5004
Number of extensions: 65484
Number of successful extensions: 220
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 198
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 218
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 329179816
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)